Intra-Articular Adhesion Reduction after Knee Surgery in Rabbits by Calcium Channel Blockers

Li, Yang; Ma, Xin; Yu, Peng; Wang, Shusen

doi:10.12659/msm.892957

Cited by 11 publications

(6 citation statements)

References 24 publications

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“…Although the effects of MMC in preventing collagen synthesis and scar formation were proved in the aforementioned studies, due to the toxicity of MMC, some researchers doubted that MMC would lead to an increased rate of infection [ 22 ]. A recent study provided clear evidence that all-trans retinoic acid (ATRA) prevents PF through the NF-κB signaling pathway [ 15 ] and another study showed the ability of azithromycin to prevent PF [ 7 ]. In the present study we found a similar effect of RPM in prevention of PF, as reflected by Rydell score and the anti-inflammatory and anti-fibrotic abilities of RPM also were as good as ATRA and azithromycin.…”

Section: Discussionmentioning

confidence: 99%

Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study

et al. 2015

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BackgroundPeridural fibrosis (PF) is a normal complication after lumbar surgery. It is a challenge for both surgeons and patients. Rapamycin (RPM), a novel antibiotic with anti-proliferative and immunosuppressive properties, has been shown to be effective in preventing uncontrolled scar proliferation diseases. The object of the present research was to investigate the effects of RPM on inhibiting PF in vitro and in vivo.Material/MethodsIn vitro, the fibroblasts collected and isolated from the rat tail skin were cultured with/without RPM and cell counting was performed. In vivo, the double-blinded study was conducted in 60 healthy Wistar rats divided randomly into 3 groups: 1) RPM treatment group; 2) Vehicle treatment group; 3) Control group. Rats underwent a L1-L2 level laminectomy with a satisfactory anesthetization. Four weeks post-operatively, the Rydell score, histological analysis, hydroxyproline content, vimentin expressional level, and inflammatory cytokines expressional levels were assessed.ResultsIn vitro, RPM showed ability to prevent fibroblast proliferation. In vivo, the laminectomy was well tolerated by all rats, which were killed 4 weeks post-operatively. The Rydell score, histological evaluation, hydroxyproline content, vimentin expression level, and inflammatory activity showed the positive effect of RPM in preventing peridural adhesion, inhibiting fibrotic formation and collagen synthesis, and down-regulating inflammation.ConclusionsIn the present primary study, RPM showed good efficacy in preventing the proliferation of fibroblasts. RPM can prevent rat peridural adhesion through inhibiting collagen synthesis, fibroblasts proliferation, and inflammatory activity.

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Section: Discussionmentioning

confidence: 99%

Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study

et al. 2015

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“…The detailed formation mechanism of intra-articular knee adhesion was not well represented in the literature. Fibroblastic activity exerts a key role in this process [ 6 , 7 , 11 ]. Fibroblast accumulation in the surgical area is considered to be caused by the activation of inflammatory activity, at which time the fibroblasts synthesize collagen and generate collagenous fibers.…”

Section: Discussionmentioning

confidence: 99%

All-Trans Retinoic Acid Reduces Joint Adhesion Formation: An Experimental Study in Rats

et al. 2015

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BackgroundIntra-articular adhesion is a common complication in post-surgical knees. The formation of post-surgical joint adhesion could lead to serious conditions. All-trans retinoic acid (ATRA) is a physiological metabolite of vitamin A that has a wide range of biological activities. The aim of the study was to verify the effects of (ATRA) in preventing adhesions in the post-operative rat knee.Material/MethodsEighty healthy adult male Wistar rats underwent femoral condyle-exposing surgery. After surgery, cotton pads soaked with the vehicle or various concentrations of ATRA (0.1%, 0.05%, 0.025%) were applied to the surgery site for 5 min. The post-surgical knee joints were fixed with micro-Kirschner wires in a flexed position for 4 weeks. The rats were killed 4 weeks after surgery. The effect of ATRA on the prevention of intra-articular adhesion was evaluated using histological analyses, hydroxyproline content, visual score, and inflammatory factor activity evaluation.ResultsNo obvious postoperative complications or signs of infection in the rats were observed. None of the rats died before the scheduled time. The rats in the 0.1% ATRA group showed better outcomes, as suggested by the visual scores, hydroxyproline contents, and inflammatory factors expressional levels, than the other 2 groups. The local application of 0.1% ATRA was able to suppress adhesions, collagen expression, and inflammatory activity in the post-surgical rat knees.ConclusionsIn the rat knee surgery model, the application of intra-articular ATRA was able to decrease intra-articular scar adhesion formation, collagen expression, and inflammatory activities. ATRA was found to work in a dose-dependent manner, with 0.1% being possible optimal concentration.

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“…Sterile conditions were created before surgery. The study model for intra-articular adhesion was based on previously published rat model protocols [ 4 , 7 , 8 ]. The animals were anesthetized using ketamine (50 mg/kg) and xylazine (10 mg/kg) via intra-peritoneal injection.…”

Section: Methodsmentioning

confidence: 99%

“…Assessment was based on a previously established visual score system [ 4 , 5 ]. Four weeks post-surgery, five rats were randomly selected from each group.…”

Section: Methodsmentioning

confidence: 99%

The Topical Application of Rosuvastatin in Preventing Knee Intra-Articular Adhesion in Rats

Germanov

Goryaeva

et al. 2016

Med Sci Monit

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BackgroundIntra-articular adhesion is one of the common complications of post knee surgery and injury. The formation of joint adhesion can lead to serious dysfunction. Rosuvastatin (ROS) is a new 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, with multiple biological effects. In our study, the object was to evaluate the effectiveness of ROS in the prevention of post-operative knee adhesion in rats.Material/MethodsFemoral condyle exposing surgery was performed on 45 healthy Sprague Dawley rats. Gelatin sponges soaked with 20 mg/kg of ROS, 10 mg/kg of ROS, or saline were used to cover the surgical site. The post-operative knee joints were fixed in a flexed position with micro Kirschner wires for four weeks. ROS effectiveness for treating intra-articular adhesion was determined with visual score evaluation, hydroxyproline content, histological analyses, immunohistochemistry, and inflammatory and vascular endothelial growth factors expression.ResultsThe animals’ recovery was stable after surgery. The hydroxyproline content, visual score, and inflammatory vascular growth factors expression levels suggested that, compared with the control group, the ROS treatment groups showed better outcomes. ROS prevented joint adhesion formation, collagen deposition, and vascularization at the surgical site, and also inhibited inflammatory activity post-operatively. Compared with the 10 mg/kg ROS group, the 20 mg/kg ROS group showed significantly better outcomes.ConclusionsThe local application of ROS reduced intra-articular adhesion formation, collagen deposition, and vascularization at the surgical site, and inhibited inflammatory activity post-operatively. These results suggested optimal concentration of ROS to be 20 mg/kg.

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Intra-Articular Adhesion Reduction after Knee Surgery in Rabbits by Calcium Channel Blockers

Cited by 11 publications

References 24 publications

Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study

Effects of Rapamycin on Reduction of Peridural Fibrosis: An Experimental Study

All-Trans Retinoic Acid Reduces Joint Adhesion Formation: An Experimental Study in Rats

The Topical Application of Rosuvastatin in Preventing Knee Intra-Articular Adhesion in Rats

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