The origin and survival of gastric parietal cells in the mouse

Abstract

Using microradioautography, the origin of the gastric parietal (and zymogen) cells was deduced by observing the time of appearance of labelled parietal cells in relation to the other labelled cells i n the gastric mucosa. In order to see whether the parietal cell divides in the adult mouse, time grain count curves of the labelled parietal cells were made from animals which were killed at 1-291 days after thymidine SH injection DPT). Parietal cell survival was followed by observing the disappearance of the labelled parietal cell population. Parietal cells appear to be entirely derived from other cells since a significant number of labelled parietal cells does not appear unless the animal is allowed to survive for several days. Parietal cells do not seem to undergo mitosis in the adult mouse because the time grain count curves did not shift with time. Although a few labelled parietal cells persisted in the 291 DPT animals, a large majority of the labelled parietal cells disappeared by 90 DPT and had a half life of roughly 23 days. This suggests an element of renewal for the parietal cell population.

Histogenesis of the mouse gastric mucosa, with special reference to type and distribution of proliferative cells.

“…Some authors described the occasional but temporary presence of labeled parietal cells after injection of 3H-thymldlne (CHEN and WITHERS, 1975;TAMURA and FUJITA, 1983), while others failed t0 see it (RAGINS et al, 1968;KATAOKA, 1970;WILLEMS et al, 1972;YEOMANS et al, 1976). In the present study, labeled immature parietal cells were rarely found in the adult mouse 1 hr after the injection of 3H-thymidine.…”

Self-Replication of Enterochromaffin-Like Cells in the Mouse Stomach

“…The latter cells are known to differentiate into surface mucous cells as well as into parietal cells [33], The fact that proliferating ECL cells were observed predominantly in the lower parts of the oxyntic mucosa suggests that ECL cells proliferate independently from the progenitor cells. However, labeling of immature ECL cells or ECL cell progeni tors might be difficult to detect.…”

Fine structural aspects on the renewal and development of surface mucous cells and glandular cells of the gastric body of the adult golden hamster.

“…However, many reports have dealt with the absence of mitotic activity in the parietal cells (HUNT, 1957;HUNT and HUNT, 1962;LIPKIN et al, 1963;RAGINS et al, 1968;LAWSON, 1970;WATTEL and GEUZE, 1977) except for a few (CHEN and WITHERS, 1975). There are also some papers emphasizing that the parietal cells are transformed from other kinds of cells such as surface mucous cells (CORPRON,1966;WINBORN et al, 1974) or mucous neck cells (RAGINS et al, 1968). Detailed observations using electron microscopic autoradiograp y of 3H-TdR are necessary to solve these problems.…”