The Ovarian Cycle of the Bitch: Plasma Estrogen, LH and Progesterone

Concannon, Patrick W.; Hansel, William; Visek, Willard J.

doi:10.1095/biolreprod13.1.112

Cited by 275 publications

(172 citation statements)

References 24 publications

Supporting

Mentioning

134

Contrasting

Unclassified

Order By: Relevance

“…The appearance of an extra LH release 4 days before the preovulatory LH peak found here in one bitch accords with a previous study (Chakraborty, 1987), although this extra LH peak was only detected with the normal incubation assay. Mean concentrations of preovulatory LH peaks were similar to those reported by Boyns et ai (1972), Concannon et al (1975) and Fernandes et ai (1987), but were lower than those observed by Smith & McDonald (1974), Nett et al (1975), Olson et al (1982) and Chakraborty (1987). Because the duration of the preovulatory increase in LH (Nett et ai, 1975;Concannon et ai, 1975) is 18-48 h, it would appear that the collection of blood at 2-day intervals would be frequent enough to record the LH peaks.…”

Section: Discussionsupporting

confidence: 87%

A rapid radioimmunoassay for determining plasma concentrations of LH in dogs

Madej

Linde‐Forsberg²

1991

Reproduction

View full text Add to dashboard Cite

Sepharose bead suspension containing anti-gamma-globulin, followed by incubation at room temperature for 30 min. The minimum detectable concentration in this assay, calculated from the precision profile, was 1\m=.\5 \ g=m\ g/ l . The amount of dog LH needed to cause 50% reduction of the initial binding was 1\m=.\57\ m=+-\0\m=.\13 ng/tube (15\m=.\7 \g=m\g/l for 100-\g=m\l samples). Daily blood samples were collected in heparinized tubes from the cephalic vein of 5 pointer and 7 beagle bitches from the onset of pro-oestrus until 3\p=n-\4 days after either the last mating or artificial insemination with frozen semen or until metoestrus. Samples were assayed for LH content by the short and normal incubations as well as for progesterone and oestradiol-17\g=b\ content. In all bitches plasma concentrations of progesterone increased rapidly within 1 week after the LH peak which indicates that they had ovulated. Comparison of the short (1\m=.\5h) with the normal (24 h) incubation system resulted in a regression equation: y = 1\m=.\0+ 0\m=.\7x (r = 0\m=.\95, n = 153 samples). The preovulatory LH peak averaged 10\m=.\4\m=+-\1\m=.\1and 11\m=.\9 \ m=+-\ 1 \ m=. \ 7 \g=m\g/l in the short and normal incubation assay, respectively. We suggest that this rapid LH assay can be used in practice to define the optimum time for mating and artificial insemination of the bitch or to study physiological aspects of the ovulatory period.

show abstract

Section: Discussionsupporting

confidence: 87%

A rapid radioimmunoassay for determining plasma concentrations of LH in dogs

Madej

Linde‐Forsberg²

1991

Reproduction

View full text Add to dashboard Cite

show abstract

“…During pregnancy the progesterone concentrations remain maximal up to about Day 35 before waning steadily to give basal levels by the time of parturition or during the early stages of lactation (Austad, Lunde &Sjaastad, 1976;Concannon et al, 1975;Hadley, 1975 b;Smith & McDonald, 1974;Jones, Boyns, Bell, Christie & Parkes, 1973c;Masken, 1972;Christie, Bell, Horth & Palmer, 1971 ;Bell, Christie & YoungLai, 1971). Jones et al (1976) found increased plasma prolactin levels on the last day of pro-oestrus, but we were unable to find any clear changes in serum prolactin concentrations with thestageof the cycle.…”

Section: Pregnancymentioning

confidence: 55%

“…The oestrous cycle in the dog is characterized principally by increasing peripheral blood oestrogen levels during pro-oestrus, followed by a surge in LH concentrations in late pro-oestrus when serum progesterone concentrations start to increase to reach maximum levels in early metoestrus (Concannon et al, 1975 ;Nett étal., 1975 ;Hadley, 1975a;Jones et al, 1973b;Masken, 1972); the increased oestro¬ gen and LH concentrations decline within 1-4 days of ovulation to basal levels for the rest of the cycle. During pregnancy the progesterone concentrations remain maximal up to about Day 35 before waning steadily to give basal levels by the time of parturition or during the early stages of lactation (Austad, Lunde &Sjaastad, 1976;Concannon et al, 1975;Hadley, 1975 b;Smith & McDonald, 1974;Jones, Boyns, Bell, Christie & Parkes, 1973c;Masken, 1972;Christie, Bell, Horth & Palmer, 1971 ;Bell, Christie & YoungLai, 1971).…”

Section: Pregnancymentioning

confidence: 99%

“…Several recent studies of the concentrations of oestrogens, progesterone and LH during the oestrous cycle and pregnancy of the dog (Concannon, Hansel & Visek, 1975;Nett et al, 1975;Smith & McDonald, 1974;Masken, 1972) have added considerably to our understanding of canine repro¬ ductive physiology. Such understanding is important because animal testing requirements for hor¬ monal contraceptives and for progestagens and oestrogens used over long periods for non-contra¬ ceptive purposes include long-term toxicity studies in the dog.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serum oestrogen, progesterone and prolactin concentrations in cyclic, pregnant and lactating beagle dogs

Gräf¹

1978

Reproduction

View full text Add to dashboard Cite

Summary. No correlations between the three hormones measured were found in any of the reproductive states examined. In 2 out of 8 dogs a negative correlation between serum progesterone and prolactin levels was found at Day 11 and Day 15, respectively (Day 0 = first day of copulation). In all of the 8 animals in which the progesterone and prolactin concentrations were measured in the same samples in late pregnancy, a negatively correlated overall pattern was obtained between both hormone profiles. No correlation between serum oestrogen and prolactin levels was found.It is concluded that the proliferative mammary gland changes which are induced in female dogs during long-term toxicity testing with progestagens are unlikely to be related to prolactin or oestrogen synthesis and/or secretion.

show abstract

“…In the dog, the lifespan of the corpus luteum varies with the individual, but it does not differ between pregnant and non-pregnant animals (2). Little information is available on the regulation of the corpus luteum lifespan in the dog, but uterine factors may be involved (3,4).…”

Section: Introductionmentioning

confidence: 99%

Evidence for the non‐involvement of the uterus in the lifespan of the corpus luteum in the cyclic dog

Okkens

Dieleman

Bevers

et al. 1985

Veterinary Quarterly

View full text Add to dashboard Cite

SUMMARY Progesterone levels in peripheral blood of dogs were analysed during the cycle in which hysterectomy (n= 5) or sham surgery (n = 3) was performed as well as during the cycle of dogs (n = 5) hysterectomized at least one year prior to this study; the data were compared with the findings in control dogs (n=3). The averages of the duration of the luteal period observed in the three experimental groups were not significantly different from those of control dogs. Immediately after surgery, the progesterone level decreased from 25 to 50% of the presurgical level, but returned to presurgical level in about four days. Prolactin levels were elevated for about 30 h after surgery. Nevertheless, the averages of the mean prolactin levels for each animal during the luteal period of the experimental groups were not significantly different from those of control dogs. It is concluded that in the dog, the uterus is not involved in the lifespan of the cyclic corpus luteum.

show abstract

The Ovarian Cycle of the Bitch: Plasma Estrogen, LH and Progesterone

Cited by 275 publications

References 24 publications

A rapid radioimmunoassay for determining plasma concentrations of LH in dogs

A rapid radioimmunoassay for determining plasma concentrations of LH in dogs

Serum oestrogen, progesterone and prolactin concentrations in cyclic, pregnant and lactating beagle dogs

Evidence for the non‐involvement of the uterus in the lifespan of the corpus luteum in the cyclic dog

Contact Info

Product

Resources

About