The oxidative stress of hyperglycemia and the inflammatory process in endothelial cells

Muniandy, Sekaran; Qvist, Rajes; Yan, Gracie Ong Siok; Bee, Chook Jack; Chu, Yiaw Koon; Rayappan, Arokiasamy Vinsent

doi:10.2152/jmi.56.6

Cited by 23 publications

(14 citation statements)

References 19 publications

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“…Furthermore, High glucose stress was used to induce insulin resistance in HUVEC cells [23]. High glucose concentration directly contributes to endothelial dysfunction [24], amplified monocyte adhesion and upregulated P-selectin expression [25]. We found that expression of miR-492 was reduced significantly, whereas the resistin was increased at mRNA and protein levels, similar to the results in aortas endothelial tissues from apoE-null mice.…”

Section: Discussionsupporting

confidence: 76%

MicroRNA-492 reverses high glucose-induced insulin resistance in HUVEC cells through targeting resistin

et al. 2014

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The development of atherosclerosis (AS) is a multifactorial process, in which elevated plasma resistin (a key factor leading to insulin resistance) levels play an important role. Emerging evidence indicate that microRNAs (miRNAs) are involved in AS; However, the regulation and function of miRNAs in response to AS remain poorly understood. Our study analyzed the effects of miR-492 on insulin resistance, endothelial activation, and resistin expression in apoE knock-out mice and human umbilical vein endothelial cells after high-glucose treatment and miR-492 mimics transfection. We also investigated the underlying molecular mechanisms. Our results showed that high glucose stress induced a significant decrease in miR-492 expression, with a remarkable upregulation of resistin expression. We then identified resistin as a novel direct target of miR-492 using 3′-UTR luciferase reporter assay. Histopathologic examination demonstrated that upregulation of miR-492 attenuated endothelial cells migration and lipid accumulation induced by high glucose stress. Further investigation demonstrated that the upregulation of p-STAT3, SOCS, and P-selectin activation induced by high glucose stress was attenuated by upregulation of miR-492. Together, our findings indicate that miR-492 contributes to insulin resistance and endothelial dysfunction induced by high glucose, via directly downregulating resistin expression, and involving STAT3 phosphorylation, SOCS, and P-selectin activation.

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Section: Discussionsupporting

confidence: 76%

MicroRNA-492 reverses high glucose-induced insulin resistance in HUVEC cells through targeting resistin

et al. 2014

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“…The increased oxidative damage may be a consequence of hyperglycemia and hyperlipidemia as discussed elsewhere [4, 6, 7]. …”

Section: Introductionmentioning

confidence: 99%

Effect of Dietaryn-3Polyunsaturated Fatty Acids on Oxidant/Antioxidant Status in Macrosomic Offspring of Diabetic Rats

Guermouche

Soulimane-Mokhtari

Bouanane

et al. 2014

BioMed Research International

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The aim of this work was to determine the effect of dietary n − 3 PUFA on oxidant/antioxidant status, in vitro very low and low density lipoprotein (VLDL-LDL), and VLDL-LDL-fatty acid composition in macrosomic pups of diabetic mothers. We hypothesized that n − 3 PUFA would improve oxidative stress in macrosomia. Diabetes was induced in female Wistar rats fed with the ISIO diet (control) or with the EPAX diet (enriched in n − 3 PUFAs), by streptozotocin. The macrosomic pups were killed at birth (day 0) and at adulthood (day 90). Lipid parameters and VLDL-LDL-fatty acid composition were investigated. The oxidant/antioxidant status was determined by measuring plasma oxygen radical absorbance capacity (ORAC), hydroperoxides, carbonyl proteins, and VLDL-LDL oxidation. Macrosomic rats of ISIO fed diabetic mothers showed an increase in plasma and VLDL-LDL-triglycerides and VLDL-LDL-cholesterol levels and altered VLDL-LDL-fatty acid composition. Plasma ORAC was low with high hydroperoxide and carbonyl protein levels. The in vitro oxidizability of VLDL-LDL was enhanced in these macrosomic rats. The EPAX diet corrected lipid parameters and improved oxidant/antioxidant status but increased VLDL-LDL susceptibility to oxidation. Macrosomia is associated with lipid abnormalities and oxidative stress. n − 3 PUFA exerts favorable effects on lipid metabolism and on the oxidant/antioxidant status of macrosomic rats. However, there are no evident effects on VLDL-LDL oxidation.

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“…Hyperglycemia increases the production of reactive oxygen species in cells and stimulates the release of the potent proinflammatory cytokines such as IL-8 and TNF-alpha. 2 Several recent studies have shown that impaired fasting glucose (IFG) in diabetes is a risk factor for arterial stiffness [3][4][5][6][7][8] and CVD, 9 but the impact of a slightly high-normal glucose level remains controversial and there are few studies to investigate the relationship between high-normal FPG and increased arterial stiffness. 8 The precise mechanisms by which the level causes CVD and mortality remain unelucidated.…”

Section: Introductionmentioning

confidence: 99%

A slightly high-normal glucose level is associated with increased arterial stiffness in Japanese community-dwelling persons with pre-diabetes

et al. 2013

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Impaired fasting glucose (IFG) in diabetes is a risk factor for arterial stiffness and cardiovascular disease (CVD), but the impact of a slightly high-normal glucose level remains controversial. We investigated whether slightly high-normal fasting plasma glucose (FPG) was independently associated with arterial stiffness in non-diabetic community-dwelling persons. We recruited 114 men aged 69 ± 9 years (range 40-89) and 208 women aged 68 ± 7 years (range 36-84) during their annual health examination in a single community. Arterial stiffness was evaluated by the mean of the right and left brachial-ankle pulse wave velocity (ba-PWV). Age-adjusted ba-PWV increased significantly from the lowest to the highest FPG group (p < 0.001). Multiple linear regression analyses for ba-PWV revealed that FPG levels (β=0.154, p < 0.001) were independently and significantly associated with ba-PWV. The multivariate-adjusted ba-PWV value of the high-normal glucose group (Group 3: 95-100 mg/dL) was significantly higher than that of the lowest normal glucose group (Group 1: 52-89 mg/dL) (p=0.021), and the ba-PWV value in the IFG group (Group 4: 101-125 mg/dL) was significantly higher than those of the normal glucose groups (Group 1: <90 mg/dL; Group 2: 90-94 mg/dL) (p<0.001 and p=0.009, respectively). Slightly high-normal glucose levels were associated with arterial stiffness in Japanese communitydwelling persons.

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The oxidative stress of hyperglycemia and the inflammatory process in endothelial cells

Cited by 23 publications

References 19 publications

MicroRNA-492 reverses high glucose-induced insulin resistance in HUVEC cells through targeting resistin

MicroRNA-492 reverses high glucose-induced insulin resistance in HUVEC cells through targeting resistin

Effect of Dietaryn-3Polyunsaturated Fatty Acids on Oxidant/Antioxidant Status in Macrosomic Offspring of Diabetic Rats

A slightly high-normal glucose level is associated with increased arterial stiffness in Japanese community-dwelling persons with pre-diabetes

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