The parthenogenetic activation of canine oocytes with Ca-EDTA by various culture periods and concentrations

Lee, Sang Ryeul; Kim, Jae Wook; Kim, Bong Soo; Kim, Myoung Ok; Kim, Sung Hyun; Yoo, Dong-Hoon; Shin, Mi-Jung; Lee, Sanggyu; Park, Young Sik; Park, Yong Bok; Ha, Jung‐Hong; Ryoo, Zae Young

doi:10.1016/j.theriogenology.2006.10.002

Cited by 10 publications

(13 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The study of parthenogenetic activation of canine oocytes has not been investigated to date. However, we showed for the first time that parthenogenetic induction by Ca‐EDTA treatment demonstrated approximately 8% of pronuclear formation (Lee et al. 2007a,b).…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Effects of Gonadotropins on In Vitro Maturation and of Electrical Stimulation on Parthenogenesis of Canine Oocytes

Kim

Lee

Hyun

et al. 2010

Reprod Domestic Animals

View full text Add to dashboard Cite

The objective of this study was to determine the effects of gonadotropins on in vitro maturation (IVM) and electrical stimulation on the parthenogenesis of canine oocytes. In experiment I, cumulus oocyte complexes were collected from ovaries at a random phase of the oestrus cycle and cultured on maturation medium treated with hCG or eCG for 48 or 72 h. There were no significant differences in the effects on the metaphase II (MII) rate between the hCG and eCG treatment groups over 48 h (5.4% vs 5.5%). The MII rate in the co-treatment group of hCG and eCG for 48 h was higher than in each hormone treated group (15.5%, p < 0.05). In experiment 2, the parthenogenetic effect on oocyte development, at various electrical field strengths (1.0, 1.5, 2.0 kV/cm DC) for 60 or 80 mus with a single DC pulse after IVM on the co-treatment of hCG and eCG, was examined. The rate of pronuclear formation (37.1%) in electrical activation at 1.5 kV/60 mus without cytochalasin B (CB) was higher than that of oocytes activated in the other groups (p < 0.05). However, we did not observe the cleavage stages. Also, CB did not influence parthenogenesis of canine oocytes. The results showed that the pronucleus formation rate, indicative of the parthenogenesis start point, could be increased by electrical stimulation. Therefore, these results can provide important data for the parthenogenesis of canine oocytes and suggest the probability of parthenogenesis in canines.

show abstract

Section: Discussionmentioning

confidence: 93%

“…In addition, the effect of gonadotropin on the MII rate of oocytes during all culture periods was not beneficial. On the contrary, short‐term or temporary exposure of oocytes to FSH or LH may support nuclear maturation (Lee et al. 2007a,b).…”

Section: Discussionmentioning

confidence: 99%

Effects of Gonadotropins on In Vitro Maturation and of Electrical Stimulation on Parthenogenesis of Canine Oocytes

Kim

Lee

Hyun

et al. 2010

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Meiotic maturation of mammalian oocytes arrested at the metaphase II (MII) stage spontaneously and sperm-induced activation completed nuclear maturation. Resumption of meiosis without sperm penetration into the ooplasm is generally referred to as activation or parthenogenesis [18]. In in vivo canine oocytes, spermatozoa already present around COCs before the initiation of maturation resulted in completion of nuclear maturation at day 2-3 after ovulation.…”

Section: Discussionmentioning

confidence: 99%

Influence of Parthenogenetic Activation on Nuclear Maturation of Canine Oocytes

et al. 2010

View full text Add to dashboard Cite

This study was designed to evaluate the nuclear maturation and maturation promoting factor (MPF) level at different maturation times, and the effect of parthenogenetic activation on nuclear maturation in canine oocytes. Cumulus-oocyte complexes (COCs) were matured in TCM-199 supplemented with 10% fetal bovine serum, hormones, 0.57 mM cysteine, and 10 ng/ml epidermal growth factor for 72 hr at 38.5 degrees C. In Experiment 1, COCs at 0, 24, 48 and 72 hr of culture were assessed for nuclear maturation and MPF levels using histone H1 kinase activity assay. A significantly higher rate of oocytes at 72 hr than 0, 24 and 48 hr of culture developed to metaphase I-anaphase I and metaphase II. Relative abundance of histone H1 kinase activity of oocytes matured for 48 hr increased to ~1.5 x, with a marked increase to approximately 2.5 x for 72 hr, significantly higher than others. In Experiment 2, oocytes matured for 48 hr were parthenogenetically activated with 5 microm ionomycin for 5 min (Group 1) and followed by 10 microg/ml cycloheximide for 3 hr (Group 2), or no treatment (Control). Oocytes were then cultured for 24 hr and assessed for nuclear maturation. A significantly higher rate of oocytes in Group 1 developed to metaphase II than in Group 2 and the control. These results indicated that ionomycin treatment at 48 hr of in vitro maturation had a positive influence on oocyte progression to the metaphase II stage.

show abstract

“…These were transported to laboratory within 3 h in a warm (38.5°C) physiological saline solution (PSS) composed of 0.9% sodium chloride, 0.1 mg/ml streptomycin sulfate and 0.06 mg/ml potassium penicillin G. When ovaries were removed from the organs, these were washed three times with fresh PSS to remove blood. Cumulus–oocyte complexes (COCs) were collected from ovaries with the method used by Lee et al. (2007).…”

Section: Methodsmentioning

confidence: 99%

“…To induce parthenogenetic activation to canine oocytes, we investigated for the first time parthenogenetic activation of oocytes treated with Ca‐EDTA (Lee et al. 2007).…”

Section: Introductionmentioning

confidence: 99%

Parthenogenetic Induction of Canine Oocytes by Electrical Stimulation and Ca‐EDTA

Lee

Kim

et al. 2009

Reprod Domestic Animals

View full text Add to dashboard Cite

In this study, we investigated parthenogenetic induction of canine oocytes by electrical stimulation following Ca-EDTA treatment. Oocyte maturation, parthenogenetic development, and cleavage rate in canine after various electrical stimulations (1.5, 1.8, 2.1 kV/cm) for 50 mus with single DC pulse following 1 mM Ca-EDTA treatment were investigated. In oocyte activated electrically at the voltage of 1.5 kV/cm after 1 mM Ca-EDTA treatment, the rate of pronucleus and two-cell was 4.1% and 2.7%, respectively. Although electrical stimulation could parthenogenetically induce immature oocyte to cleavage stage, degeneration rate in all experimental groups was more than 60%. This means that electrical stimulation after Ca-EDTA treatment could cause canine oocytes to be degenerated. However, two-cell in canine oocyte by parthenogenesis was for the first time induced. Therefore, we suggested that electrical stimulation for canine oocytes could induce parthenogenetically early embryonic cleavage. This result can be used as a basic data for parthenogenesis study in canine. Also, to perform more developed embryonic development, further study to parthenogenesis in canine need to be developed.

show abstract

The parthenogenetic activation of canine oocytes with Ca-EDTA by various culture periods and concentrations

Cited by 10 publications

References 35 publications

Effects of Gonadotropins on In Vitro Maturation and of Electrical Stimulation on Parthenogenesis of Canine Oocytes

Effects of Gonadotropins on In Vitro Maturation and of Electrical Stimulation on Parthenogenesis of Canine Oocytes

Influence of Parthenogenetic Activation on Nuclear Maturation of Canine Oocytes

Parthenogenetic Induction of Canine Oocytes by Electrical Stimulation and Ca‐EDTA

Contact Info

Product

Resources

About