The Pathogenesis of Contact Lens-Associated Pseudomonas aeruginosa Corneal Ulceration I. The Effect of Contact Lens Coatings on Adherence of Pseudomonas aeruginosa to Soft Contact Lenses

Stern, George A.; Zam, Z. Suzanne

doi:10.1097/00003226-198605010-00010

“…Mayo et al (15) determined that nonmucoid strains of Pseudomonas aeruginosa isolated from corneal ulcers and home-prepared saline of a patient generally were the same strain. Other investigations have also concluded that lens solutions were probable vectors for eye-associated infections (24,26). Lenses that have been soaked in contaminated solutions may harbor adhered bacteria.…”

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confidence: 99%

Adherence of Pseudomonas aeruginosa to hydrophilic contact lenses and other substrata

Miller¹,

Ahearn²

1987

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Nonmucoid Pseudomonas aeruginosa isolated from corneal ulcers and contact lens cases and solutions were examined for their ability to adhere to polystyrene, glass, and hydrophilic contact lenses of varying water content and polymer composition. Adherence to the various substrates was strain specific. Adherence also was influenced by hydrophobicity and chemical composition of the substratum, as well as pH and electrolyte concentration. An extracellular polymeric adhesive appeared to be involved in the firm attachment of cells to soft contact lenses.

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“…Mayo et al (15) determined that nonmucoid strains of Pseudomonas aeruginosa isolated from corneal ulcers and home-prepared saline of a patient generally were the same strain. Other investigations have also concluded that lens solutions were probable vectors for eye-associated infections (24,26). Lenses that have been soaked in contaminated solutions may harbor adhered bacteria.…”

mentioning

confidence: 99%

“…Lenses that have been soaked in contaminated solutions may harbor adhered bacteria. Because most contact lenses result in changes in the cornea that could predispose to infection, bacteria harbored on the lens present a risk (2,24).…”

mentioning

confidence: 99%

The Adherence of Pseudomonas aeruginosa to Hydrophilic Contact Lenses and Other Substrata

Miller¹,

Wilson²,

Ahearn³

1987

Cornea

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Nonmucoid Pseudomonas aeruginosa isolated from corneal ulcers and contact lens cases and solutions were examined for their ability to adhere to polystyrene, glass, and hydrophilic contact lenses of varying water content and polymer composition. Adherence to the various substrates was strain specific. Adherence also was influenced by hydrophobicity and chemical composition of the substratum, as well as pH and electrolyte concentration. An extracellular polymeric adhesive appeared to be involved in the firm attachment of cells to soft contact lenses.Recently, an apparently increased incidence of Pseudomonas keratitis has been associated with hydrophilic contact lens wear (1,6,12,16,18,19,25). Mayo et al. (15) determined that nonmucoid strains of Pseudomonas aeruginosa isolated from corneal ulcers and home-prepared saline of a patient generally were the same strain. Other investigations have also concluded that lens solutions were probable vectors for eye-associated infections (24,26). Lenses that have been soaked in contaminated solutions may harbor adhered bacteria. Because most contact lenses result in changes in the cornea that could predispose to infection, bacteria harbored on the lens present a risk (2, 24).Few workers have examined the adherence of pseudomonads to hydrophilic contact lens polymers. Pringle and Fletcher (21) reported that a freshwater isolate of P. fluorescens demonstrated decreased adherence with increased water content of a hydrogel polymer used for contact lenses. Dart and Badenoch (4) demonstrated that P. aeruginosa adhering to hard contact lenses showed higher attachment numbers in comparison to soft, more hydrophilic lens material. These and other studies (9,11,13) have demonstrated a relationship between the hydrophobicity of a surface and microbial adherence.A number of workers have examined the adherence of Pseudomonas species to substrata, such as polystyrene (10, 17, 20, 22), glass (14, 27), and stainless steel (23). Bacteria prefer to attach to low-energy, hydrophobic surfaces such as polystyrene rather than to high-energy, hydrophilic surfaces such as glass. Most studies support a theory of "irreversible sorption," which is a time-dependent process, facilitated by the synthesis of an extracellular polymeric material that bridges the bacterial and substratum surface (3,7,8,14).Factors affecting the adherence of P. aeruginosa to contact lenses of varying polymer composition have not been examined. We have evaluated the interactions of eyeassociated isolates of nonmucoid P. aeruginosa with hydrogels under varying physiological and physicochemical conditions. * Corresponding author. MATERIALS AND METHODSOrganisms and culture conditions. Twenty cultures of nonmucoid P. aeruginosa isolated by Mayo et al. (15) from corneal ulcers and contact lens solutions and cases were grown overnight at 25°C on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.). Isolated colonies were used to inoculate 200 ml of tryptic soy broth (Difco Laboratories, Detroit, Mich.), pH 7.3, that ...

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“…The latter study also concluded that a mucin solution and mucin-coated lenses inhibited adherence of P. aeruginosa. However, lenses coated with mucin were found to enhance adherence of an isolate of P. aeruginosa (28,29).…”

Section: Discussionmentioning

confidence: 99%

Effects of protein, mucin, and human tears on adherence of Pseudomonas aeruginosa to hydrophilic contact lenses

Miller

¹

,

Wilson

²

,

Ahearn

³

1988

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Pseudomonas aeruginosa isolated from a corneal ulcer was examined for its ability to adhere to hydrophilic contact lenses with adsorbed organic films. Generally, adherence was enhanced by mucin, lactoferrin, lysozyme, immunoglobulin A, bovine serum albumin, and a mixture of these macromolecules. The water content of the lenses also influenced bacterial adherence and protein adsorption. Adherence to lenses coated with human tear films was more complex; both enhanced and inhibited adherence were observed on lenses obtained from different individuals.

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The Pathogenesis of Contact Lens-Associated Pseudomonas aeruginosa Corneal Ulceration I. The Effect of Contact Lens Coatings on Adherence of Pseudomonas aeruginosa to Soft Contact Lenses

Cited by 44 publications

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Adherence of Pseudomonas aeruginosa to hydrophilic contact lenses and other substrata

Adherence of Pseudomonas aeruginosa to hydrophilic contact lenses and other substrata

The Adherence of Pseudomonas aeruginosa to Hydrophilic Contact Lenses and Other Substrata

Effects of protein, mucin, and human tears on adherence of Pseudomonas aeruginosa to hydrophilic contact lenses

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