Abstract:Background: Patients` satisfaction with nursing care provided is a good indicator for improving quality of care.Aims: Assess nurses` knowledge and practice regarding lumbar puncture and investigate patients' satisfaction with nursing care pre and post application of nursing instructions brochure. Research design: Quasi-experimental. Subjects and methods: All available nurses (22) in neurosurgery department at Assiut Neurological, Psychiatric and Neurosurgery University Hospital. Also, a sample of 60 adult patients undergoing lumbar puncture was included and divided into two groups (A and B). Group A (30 patients before application of nursing instructions brochure) and group B (30 patients after application of nursing instructions brochure). Nurses' knowledge and practice were assessed before and after application of the nursing instructions brochure. Tools: Structured questionnaire to assess nurses` demographic data and knowledge, observation checklist for nurses` practice, patients' satisfaction with nursing care scale and nursing instructions brochure for nurses. Results: Highly significant differences were found as regarding nurses` knowledge and nurses` practice pre and post application of the nursing instructions brochure. Significant difference was found between groups (A and B) regarding patients' satisfaction with nursing care. Conclusion: Nursing instructions brochure had a significant effect on improving patients` satisfaction with nursing care. Recommendation: Patients` satisfaction with nursing care should assess periodically in every care settings.