The Indonesian sugar and ethanol industries have developed rapidly in recent years. Indonesia Biofuel Association indicated that ethanol production could reach 180 million liters per year, with average domestic consumption of 100 million L per year. Sugarcane has high potential energy, 40% transformed into alcohol, and 31% remain in the by-products as bagasse (26%) and vinasse (5%) (Del Nery et al., 2018). Vinasse is the by-product of the alcohol distillation process. The production of vinasse in a traditional alcohol factory is around 8-20 L per Liter of ethanol produced (Cabrera-Díaz et al., 2017;Joppert et al., 2017). Vinasse is characterized by a high concentration of organic matter (10-65 g BOD/l), nutrient salts (potassium (K) and sulfate), low pH (3.5-5.0), high temperature (80-90°C), and permanent dark color (brown to black) (Cabrera-Díaz et al., 2017;Joppert et al., 2017;Marafon et al., 2020). The water and soil pollution resulting from sugarcane vinasse wastewater disposal is a challenging issue that comes from the biofuel ethanol industries (Harihastuti and Marlena, 2018).Due to its high organic concentration and flowrate, sugarcane vinasse cannot be effectively treated by using conventional methods. A fullscale application that involves integrating several technologies should be applied to remove organic