The Phylogenetic Relationship Among Varieties of Lansium domesticum Correa Based on ITS rDNA Sequences

Abstract

Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containing therapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer, antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known as duku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties is nearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences of the ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogenetic relationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status of duku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNA sequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotide sequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from 18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite for Sequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragments ranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku, kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. It was determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku, kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with the similarity index value of 0.996.

Lansium domesticum—A Fruit with Multi-Benefits: Traditional Uses, Phytochemicals, Nutritional Value, and Bioactivities

“…The different parts of L. domesticum have various medicinal and non-medicinal uses in many nationalities ( Table 3 ). The peel is wealthy in non-toxic oleoresin that is utilized against diarrhea and fevers [ 8 ]. In Thailand, the peel and flesh have been used as facial toners, wash gels, and masks, as well as a skin moisturizer and whitening cream.…”

“…Thailand, Malaysia, Indonesia, and the Philippines are considered to be the main producers of L. domesticum . Additionally, the plant is cultivated in Burma, Vietnam, Puerto Rico, Sri Lanka, India, Hawaii, Surinam, and Australia [ 5 , 8 , 9 ]. L. domesticum Correa is a complicated aggregate species of different plant forms.…”

Characteristics of the Genetic Variation of a Swamp Buffalo (Bubalusbubalis) of South Sumatra Based on Polymerase Chain Reaction-Random Amplified Polymorphic DNA (PCR-RAPD)

“…Furthermore, the value of Y obtained and will be used as antilog long known bases are amplified by a primer. After lengthy bases are known and obtained binary data is expressed with 'No (1) or no (0)' [9], the data is then analyzed its genetic variation with the program SPSS (Statistical Package for the Social Sciences) ver 16.0 and presented with a form of cluster analysis, a dendogram using the coefficientsimilaritas.…”

Land suitability assessment for Lansium domesticum cultivation on agroforestry land using matching method and geographic information system

“…The fruit of L. domesticum is rich in vitamin B and phosphorus, carbohydrates, and protein (Munir et al 2018). There are many studies on L. domesticum focused on various aspects, including agroecological aspect (Apuy et al 2017;Hanum et al 2013;Isfaeni et al 2012;Mayanti et al 2011), genetic variations (Yulita 2011), chemical contents and efficacy of the parts of the plant (Marfori et al 2015;Yapp and Yap 2003;Wahyuni et al 2014;Tilaar et al 2008;Song et al 2000;Saewan et al 2006;Klungsupya et al 2015;Matsumoto et al 2018) and morphological characterization (Salim et al 2016).…”