The possible protective role of royal jelly against cisplatin-induced testicular lesions in adult albino rats

Raafat, Mona; Hamam, Ghada Galal

doi:10.1097/01.ehx.0000414840.79069.9d

Cited by 6 publications

(10 citation statements)

References 35 publications

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“…Concerning the present ultrastructural observations of the testis after RJ administration showed recovery in spermatogonia, Sertoli, primary spermatocytes, spermatids, Leydig cells except few vacuoles and moderate improvement in number of sperms these results are conchoids with results of Raafat et al [71] who illustrated that cisplatin affected on testes cells and RJ restore all changes as most seminiferous tubules were seen to be almost similar to those of the control group. Moreover, Leydig cells appeared almost like those seen in controls.…”

Section: Discussionsupporting

confidence: 86%

Protective Effect of Alpha Lipoic Acid and Royal Jelly against the Side Effects of Cyclophosphamide in Testis of Male Albino Rats

Moustafa

Ismail

Nesr

et al. 2019

Egyptian Journal of Histology

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Introduction: Cyclophosphamide (CP) is a widely used anti-cancer drug which can induce serious male infertility. Aim of the study: The present study aims at determining the protective role of alpha-lipoic acid and royal jelly in improving biochemical, histological and ultrastructural disorders in the testis of rats induced by CP. Materials and Methods: 64 rats were separated into eight groups each of 8 rats. (G1) control group, (G2) alkaline solution treated group, (G3) DMSO treated group, (G4) Alpha-lipoic acid (LA) treated group, (G5) Royal jelly (RJ) treated group, (G6) CP treated group (5 mg/kg b.wt) three days per a week for 4 weeks, (G7) CP plus LA treated group (25 mg/kg b. wt) and (G8) CP plus RJ treated group (1g/kg b. wt). Animals were sacrificed after 4 weeks. Results: CP leads to an increase in lipid peroxidation in form of malonaldehyde (MDA) and decline in superoxide dismutase (SOD) in comparison with control. It caused histopathological changes in testes of rats including vacuolation, exfoliation of germ cells in the lumina of the seminiferous tubules, and maturation arrest. Furthermore, oedema was also observed and congestion of the intertubular blood vessels. Ultrastructurally, the boundary tissue of some tubules displayed noticeable changes; it was thickened and folded. Sertoli cells displayed an increase in the number of lysosomes and their nuclei showed signs of pyknosis, cytoplasmic vacuolation. Administration of LA and RJ to CP-treated rats revealed marked improvement in the altered level of SOD and MDA activities when compared with the CP-treated group and showed improvement in histopathology and ultrastructure of the testis. Conclusion: Treatment of the cyclophosphamide with the alpha-lipoic acid-induced great regaining of the damaged testicular tissues and royal jelly induced little regaining of the damaged testicular tissues, so LA was effective in treatment with CP than RJ with CP. This enhancement because of their antioxidant properties and its scavenging abilities against active free radicals.

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Section: Discussionsupporting

confidence: 86%

Protective Effect of Alpha Lipoic Acid and Royal Jelly against the Side Effects of Cyclophosphamide in Testis of Male Albino Rats

Moustafa

Ismail

Nesr

et al. 2019

Egyptian Journal of Histology

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“…For mean diameter of seminiferous tubules, rounded transversely cut tubules were selected and two diameters of each tubule, one perpendicular to the other, were measured and the average taken [33].…”

Section: Morphometric Studymentioning

confidence: 99%

“…In order to gain insights into the mechanistic mode of action of vitamin D3 on ADAM17 protein, we have performed extensive molecular modelling studies to investigate the binding affinity of vitamin D3 toward the binding site of ADAM17 protein. In the Protein data bank (PDB), there are more than 23 crystal structures available for ADAM17 protein co-crystallized with different ligands [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43]. Thus, molecular modelling approaches were performed for all available 3D crystal structure for ADAM17 protein, and results were extracted to be compared (Table 6).…”

Section: In Silico Molecular Modelling Studymentioning

confidence: 99%

Vitamin D3 Prevents the Deleterious Effects of Testicular Torsion on Testis by Targeting miRNA-145 and ADAM17: In Silico and In Vivo Study

et al. 2021

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Testicular torsion (TT) is the most common urological emergency in children and young adults that can lead to infertility in many cases. The ischemia-reperfusion (IR) injury due to TT has been implicated in the pathogenesis of testicular damage. The main pathological mechanisms of contralateral injury after ipsilateral TT are not fully understood. In the presented study, we investigated the molecular and microscopic basis of ipsilateral and contralateral testicular injury following ipsilateral testicular torsion detorsion (T/D) and explored the possible protective role of vitamin D3. The biochemical analysis indicated that IR injury following T/D significantly decreased the activity of testicular glutathione peroxidase (GPx) enzyme, level of serum testosterone, serum inhibin B, and expression of testicular miRNA145, while increased the activity of testicular myeloperoxidase (MPO) enzyme, level of testicular malondialdehyde (MDA), level of serum antisperm-antibody (AsAb), and expression of ADAM-17. The histological and semen analysis revealed that torsion of the testis caused damages on different tissues in testis. Interestingly, administration of vitamin D3 prior to the IR injury reversed the deterioration effect of IR injury on the testicular tissues as indicated by biochemical and histological analysis which revealed normal appearance of the seminiferous tubules with an apparent decrease in collagen fiber deposition in both ipsilateral and contralateral testes. Our results revealed that the protective effect of vitamin D3 treatment could be attributed to target miRNA145 and ADAM17 protein. To further investigate these findings, we performed a detailed molecular modelling study in order to explore the binding affinity of vitamin D3 toward ADAM17 protein. Our results revealed that vitamin D3 has the ability to bind to the active site of ADAM17 protein via a set of hydrophobic and hydrophilic interactions with high docking score. In conclusion, this study highlights the protective pharmacological application of vitamin D3 to ameliorate the damages of testicular T/D on the testicular tissues via targeting miRNA145 and ADAM17 protein.

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“…[ 9 ] Group III (RJ group) received intragastric RJ dispersed in distilled water, orally via gavage at a dose of 100 mg/kg/day for 10 days. [ 5 ] Group IV (Cis group) received a single IP injection of Cis at a dose of 7 mg/kg. [ 9 ] Group V (Cis + Nano Se group) received a single IP injection of Cis at a dose of 7 mg/kg followed by intragastric administration of Nano Selenium dispersed in distilled water, orally via gavage at a dose of 2 mg/kg/day for 10 days.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

“…Interestingly, growing evidences revealed that combination therapy of Cis with antioxidants can be beneficial to overcome this special reproductive toxicity. [ 1 ] Some antioxidants were shown to protect against Cis toxicity such as curcumin,[ 3 ] ginger,[ 4 ] royal jelly (RJ),[ 5 ] and selenium. [ 6 ]…”

Section: Introductionmentioning

confidence: 99%

The Effect of Selenium Nanoparticles versus Royal Jelly against Cisplatin-Induced Testicular Toxicity in Adult Male Albino Rats

Mohammed

Eisa²,

El-Aziz³

et al. 2022

Journal of Microscopy and Ultrastructure

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Background and Aim: Cisplatin (Cis) is a highly effective chemotherapeutic agent. However, it produces severe testicular toxicity. It was reported that some antioxidants could overcome this toxicity. Selenium nanoparticles and royal jelly (RJ) were among these reported antioxidants. Therefore, this study was designed to compare these two antioxidants in protecting the testes against Cis-induced toxicity. Materials and Methods: This study was conducted on sixty healthy adult male albino rats (weight: 200–220 g) randomized into six groups, ten animals each. Group I (control), Group II (animals received intragastric Nano Selenium), Group III (animals received intragastric RJ), Group IV (animals received an IP injection of Cis 7 mg/kg), Group V (animals received intragastric Nano Selenium, and Cis injection), and Group VI (animals received intragastric RJ and Cis injection). After 10 days, the animals were sacrificed by cervical decapitation. The testes were weighted, and specimens from the left testis were processed for histological and immunohistochemical techniques, whereas specimens from the right testes were prepared for transmission electron microscopic examination. Results: Cis-treated animals had significantly reduced weight of their testes. Light microscopic examination revealed severe histopathological changes in the germinal epithelium and Leydig cells, confirmed with electron microscopic examination. There was a significant increase in the color area percentage of Caspase-3 immunostaining of the germinal epithelium and Leydig cells, compared to that of the control group. Group II and III were similar to control group. Both Groups V and VI revealed significant preservation compared to the Cis group. Conclusion: Selenium nanoparticles and RJ partially improved testis from Cis-induced toxicity, However, there was no significant difference between both groups.

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The possible protective role of royal jelly against cisplatin-induced testicular lesions in adult albino rats

Cited by 6 publications

References 35 publications

Protective Effect of Alpha Lipoic Acid and Royal Jelly against the Side Effects of Cyclophosphamide in Testis of Male Albino Rats

Protective Effect of Alpha Lipoic Acid and Royal Jelly against the Side Effects of Cyclophosphamide in Testis of Male Albino Rats

Vitamin D3 Prevents the Deleterious Effects of Testicular Torsion on Testis by Targeting miRNA-145 and ADAM17: In Silico and In Vivo Study

The Effect of Selenium Nanoparticles versus Royal Jelly against Cisplatin-Induced Testicular Toxicity in Adult Male Albino Rats

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