The Potential of Endophytic Fungi Isolated from Leaves, Stems, Mangrove Roots Avicennia marina as a Producer of Antibiotics

Abstract

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.

Antibacterial Activity and Phytochemical Screening of The Fraction of Endophytic Fungus Derived from Sambiloto Flowers (Andrographis paniculata (Burm.f.) Nees)

“…Isolasi jamur endofitik dimulai dengan proses sterilisasi pada permukaan bunga sambiloto menggunakan natrium hipoklorit dan alkohol karena memiliki efektivitas tinggi untuk desinfeksi berbagai mikroorganisme yang ada pada permukaan bunga sambiloto. Bunga yang telah steril di tempelkan pada media PDA yang telah ditambah antibiotik sebagai kontrol negatif untuk menjadi pembanding dalam pertumbuhan jamur endofitik (Lestari et al, 2019). Media PDA pada isolasi jamur endofitik digunakan karena merupakan salah satu media yang baik untuk jamur.…”