The potential of extracellular microvesicles of mesenchymal stromal cells in obstetrics

Пекарев, О. Г.; Pekareva, E.O.; Mayborodin, I.V.; Silachev, D. N.; Баранов, И.И.; Pozdnyakov, I. M.; Bushueva, N. S.; Novikov, A. M.; Сухих, Г. Т.

doi:10.1080/14767058.2021.1951213

Cited by 5 publications

(9 citation statements)

References 10 publications

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Section: Discussionmentioning

confidence: 99%

“…Aside from procedural interventions, recent research also suggests potential adjunct applications of PRP and MSC to reduce the incidence and severity of uterine niches [41][42][43][44][45]. PRP and MSC were examined as intraoperative injections between the decidua and myometrium at the uterine closure site [41,42,45]. PRP, in particular, has demonstrated potential in endometrial regeneration and management of Asherman's syndrome, in addition to improved superficial scar healing from C-sections [36,37].…”

Section: Discussionmentioning

confidence: 99%

“…Early clinical trials have demonstrated a good safety profile, limited myometrial regeneration, and reduction of post-operative inflammatory conditions in C-sections with myometrial MSC injections [43][44][45]. The reduction of inflammatory complications may be relevant as the reduction of adhesion formation secondary to inflammation may also prevent future niche formation.…”

Section: Discussionmentioning

confidence: 99%

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Intra-operative Guidelines for the Prevention of Uterine Niche Formation in Cesarean Sections: A Review

Backer,

Khanna,

Sadr

et al. 2023

Cureus

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Formation of a uterine niche following a C-section can predispose the patient to future obstetric complications such as dehiscence, uterine rupture, ectopic pregnancy, and placenta accreta. The significant morbidity and mortality of these complications along with increasing C-section rates emphasizes the importance of prevention. However, there are no clear guidelines on intra-operative protocol to prevent postpartum niche formation. Besides surgical technique, the novel use of platelet-rich plasma (PRP) and mesenchymal stem cell (MSC) injections has demonstrated promising potential and may have applications in hysterotomy closures. The objective is to examine current research on optimal C-section procedures to prevent uterine niche formation and subsequent obstetric complications. A systematic review was conducted using PubMed and Google Scholar. Initial searches yielded 827 results. Inclusion criteria were human, animal, and in-vitro studies, peer-reviewed sources, and outcomes pertinent to the uterine niche. Exclusion criteria applied to articles with outcomes unrelated to myometrium and interventions outside of the intra-operative and immediate pre-/post-operative period. Based on the criteria, 41 articles were cited. Pathophysiology of uterine niche formation was associated with incisions through cervical tissue, adhesion formation, and poor approximation. Significant risk factors were low uterine incisions, advanced cervical dilatation, low station, non-closure of the peritoneum, and creation of a bladder flap. There was no consensus on uterine closure as it likely depends on surgical proficiency with the given technique, but a double-layered non-locking suture appears reliable to reduce niche severity. Recent trials indicate that intra-operative PRP/MSC injections may decrease niche incidence and severity, but more research is needed. If prevention or minimization of uterine niche is desired, the optimal C-section protocol should avoid low uterine incisions, choose uterine closure technique based on the surgeon's proficiency (double-layered non-locking is reliable), and close the peritoneum, and myometrial injection of PRP/MSC may be a useful adjunct intervention pending further clinical evidence.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Intra-operative Guidelines for the Prevention of Uterine Niche Formation in Cesarean Sections: A Review

Backer,

Khanna,

Sadr

et al. 2023

Cureus

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“…Similar to exosomes, microvesicles expose tetraspanins CD9 and CD63 on their surface [ 49 ]. The functions and therapeutic potential of MSC-derived microvesicles have been thoroughly analyzed [ 50 , 51 , 52 ].…”

Section: Msc-derived Extracellular Vesicles: Exosomes Microvesicles A...mentioning

confidence: 99%

Apoptotic MSCs and MSC-Derived Apoptotic Bodies as New Therapeutic Tools

Холоденко

Yarygin

et al. 2022

CIMB

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Over the past two decades, mesenchymal stem cells (MSCs) have shown promising therapeutic effects both in preclinical studies (in animal models of a wide range of diseases) and in clinical trials. However, the efficacy of MSC-based therapy is not always predictable. Moreover, despite the large number of studies, the mechanisms underlying the regenerative potential of MSCs are not fully elucidated. Recently, it has been reliably established that transplanted MSCs can undergo rapid apoptosis and clearance from the recipient’s body, still exhibiting therapeutic effects, especially those associated with their immunosuppressive/immunomodulating properties. The mechanisms underlying these effects can be mediated by the efferocytosis of apoptotic MSCs by host phagocytic cells. In this concise review, we briefly describe three types of MSC-generated extracellular vesicles, through which their therapeutic functions can potentially be carried out; we focused on reviewing recent data on apoptotic MSCs and MSC-derived apoptotic bodies (MSC-ApoBDs), their functions, and the mechanisms of their therapeutic effects.

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“…MSC-derived MVs also show immunomodulatory effects in inflammatory or autoimmune disorders, such as uveoretinitis (Shigemoto-Kuroda et al, 2017), graft-versus-host disease (Wang et al, 2016), and type Ⅰ diabetes (Nojehdehi et al, 2018). Cesarean delivery is a growing worldwide maternal delivery technique to reduce scarring and optimize recovery, Pekarev et al (Pekarev et al, 2021) developed a pilot study that evaluated the safety profile of MVs from mesenchymal stromal placental cells. This trial found that the intra-surgery injection of MSC-MVs was well tolerated and associated with a minimum rate of infectious post-partum complications after cesarean delivery.…”

Section: Function and Application Of Mvs In Regenerative Medicinementioning

confidence: 99%

Characteristics of culture-condition stimulated exosomes or their loaded hydrogels in comparison with other extracellular vesicles or MSC lysates

Luo

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Recently, it has become popular to study the use of extracellular vesicles (EVs) secreted by stem cells to repair damaged tissues or lost cells. Various cell types and physiological fluids release EVs, and they play an important role in cell-tocell communication. Moreover, EVs have been implicated in important processes, such as immune responses, homeostasis maintenance, coagulation, inflammation, cancer progression, angiogenesis, and antigen presentation. Thus, EVs participate in both physiological and pathological progression. The main classes of EVs include exosomes, microvesicles (MVs), and apoptotic bodies (ApoBDs). Exosomes, which carry a mass of signal molecules such as RNA, DNA, proteins, and lipids, are the most important of these EVs subsets. Currently, exosomes are generating substantial interest in the scientific community. Exosomes loaded hydrogels or under different cultural environments exhibit different properties and functions. Therefore, the exosomes obtained from different sources and conditions are worth reviewing. More importantly, no review article has compared the different EVs, such as exosomes, MVs, ApoBDs, and mesenchymal stem cell (MSC) lysates, which are special soluble substances. The differentiation between EVs and MSC lysates is a logical approach. Accordingly, this review provides an update on the latest progress in studying the roles of culture-condition stimulated exosomes or their loaded hydrogels and the differentiation between exosomes, MVs, ApoBDs, and MSC lysates. Published studies were retrieved from the PubMed ® database for review.

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The potential of extracellular microvesicles of mesenchymal stromal cells in obstetrics

Cited by 5 publications

References 10 publications

Intra-operative Guidelines for the Prevention of Uterine Niche Formation in Cesarean Sections: A Review

Intra-operative Guidelines for the Prevention of Uterine Niche Formation in Cesarean Sections: A Review

Apoptotic MSCs and MSC-Derived Apoptotic Bodies as New Therapeutic Tools

Characteristics of culture-condition stimulated exosomes or their loaded hydrogels in comparison with other extracellular vesicles or MSC lysates

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