The potential of matrix‐assisted laser desorption/ionization mass spectrometry in the quality control of water buffalo mozzarella cheese

Angeletti, Roberto; Gioacchini, Anna Maria; Seraglia, Roberta; Piro, Roberto; Traldi, Pietro

doi:10.1002/(sici)1096-9888(199806)33:6<525::aid-jms655>3.3.co;2-j

Cited by 10 publications

(23 citation statements)

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“…7 which were compared a posteriori for their discrimination performances. PR-P assays were performed directly on diluted skimmed milk samples [9][10][11][12][13][14] . On the other hand, PE-P measurements were realized on diluted low-molecular mass skimmed milk filtrates that were then straightly analyzed by MALDI-TOF-MS or extracted with concentration devices before MS analysis.…”

Section: Maldi-tof-ms Profiling Measurementsmentioning

confidence: 99%

“…MALDI-TOF-MS has the advantage of requiring small amounts of even heterogeneous samples, permitting their analysis in a very short time. In particular, detection of -lactalbumin, -lactoglobulin and 2/3-casein isoforms during protein profiling (PR-P) of whey and skimmed milk samples has been used to reveal definite species-associated milk frauds [9][10][11][12][13] . Linear regression analysis of the resulting mass spectra quantified non-declared milk material even in low amounts 13 .…”

Section: Introductionmentioning

confidence: 99%

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MALDI-TOF-MS Platform for Integrated Proteomic and Peptidomic Profiling of Milk Samples Allows Rapid Detection of Food Adulterations

Sassi

Arena

Scaloni

2015

J. Agric. Food Chem.

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Adulteration of ovine, caprine, and buffalo milks with more common bovine material occurs for economic reasons and seasonal availability. Frauds are also associated with the use of powdered milk instead of declared, fresh material. In this context, various analytical methods have been adapted to dairy science applications with the aim to evaluate adulteration of milk samples, although time-consuming, suitable only for speciation or thermal treatment analysis, or useful for a specific fraud type. An integrated MALDI-TOF-MS platform for the combined peptidomic and proteomic profiling of milk samples is here presented, which allows rapid detection of illegal adulterations due to the addition of either nondeclared bovine material to water buffalo, goat, and ovine milks or of powdered bovine milk to the fresh counterpart. Peptide and protein markers of each animal milk were identified after direct analysis of a large number of diluted skimmed and/or enriched diluted skimmed filtrate samples. In parallel, markers of thermal treatment were characterized in different types of commercial milks. Principal components scores of ad hoc prepared species- or thermal treatment-associated adulterated milk samples were subjected to partial least-squares regression, permitting a fast accurate estimate of the fraud extents in test samples at either protein and peptide level. With respect to previous reports on MALDI-TOF-MS protein profiling methodologies for milk speciation, this study extends that approach to the analysis of the thermal treatment and introduces an independent, complementary peptide profiling measurement, which integrates protein data with additional information on peptides, validating final results and ultimately broadening the method applicability.

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Section: Maldi-tof-ms Profiling Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MALDI-TOF-MS Platform for Integrated Proteomic and Peptidomic Profiling of Milk Samples Allows Rapid Detection of Food Adulterations

Sassi

Arena

Scaloni

2015

J. Agric. Food Chem.

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“…The method was applied to milk and mozzarella cheese and the ratios of the b-lactoglobulin (b-LG) variants A and B and of b-LG A and the so-called Bx peak which is only present in buffalo's milk were used for quantitative evaluation. In several studies mass spectrometry (MS) was used to assess the presence or absence of cow's milk in buffalo's milk (Angeletti, Gioacchini, Seraglia, Piro, & Traldi, 1998;Casetta, Bonner, Shushan, & Fior, 1992;Cozzolino, Passalacqua, Salemi, & Garozzo, 2002;Cozzolino et al, 2001) and mozzarella (Angeletti et al, 1998;Cozzolino et al, 2002) based on the characteristic molecular masses of a casein (Casetta et al, 1992), the protein X (Angeletti et al, 1998) and alactalbumin (Cozzolino et al, 2001(Cozzolino et al, , 2002. With one exception (Casetta et al, 1992), where electrospray ionisation was used, all MS investigations employed matrix assisted laser desorption/ionisation.…”

Section: Introductionmentioning

confidence: 97%

Detection of the adulteration of water buffalo milk and mozzarella with cow’s milk by liquid chromatography–mass spectrometry analysis of β-lactoglobulin variants

2010

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“…Matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is the potential tool to identify and quantify the various proteins present in mixture [9][10][11][12][13][14][15]. The protein samples are absorbed in the matrix composed of non-volatile material which is then irradiated using lasor beam which thus results in the formation of vaporized ion.…”

Section: Introductionmentioning

confidence: 99%

Identification of Bioactive Peptides in Goat Milk and Their Health Application

Sharma¹,

Rout²,

Kaushik³

et al. 2017

J Adv Dairy Res

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We have identified the bioactive peptides in goat milk using two dimensional gel electrophoresis and mass spectrometry. The milk samples were collected from three healthy breeds of goats at CIRG farms. The milk proteins were isolated using simple centrifugation and were evaluated on the basis of presence of other proteins variants using SDS PAGE and two dimensional gel electrophoresis. The protein profiles of all the milk proteins were same showing the presence of all the major caseins proteins and whey proteins whereas the two dimensional gel electrophoresis resulted gels has shown the presence of various protein spots. These protein spots were analysed under mass spectrometry and found biologically active peptides dipeptidyl peptidase, transcription factor A, Interleukin 12 subunit, oligodendrocyte transcription factor 2. In our study we have reported the biological significance of these peptides identified and their role in human health such as mitochondrial disease, brain malignancies, signaling processes of cytokines and help in generating cytotoxic lymphocyte helps enhance immunity.

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The potential of matrix‐assisted laser desorption/ionization mass spectrometry in the quality control of water buffalo mozzarella cheese

Cited by 10 publications

References 0 publications

MALDI-TOF-MS Platform for Integrated Proteomic and Peptidomic Profiling of Milk Samples Allows Rapid Detection of Food Adulterations

MALDI-TOF-MS Platform for Integrated Proteomic and Peptidomic Profiling of Milk Samples Allows Rapid Detection of Food Adulterations

Detection of the adulteration of water buffalo milk and mozzarella with cow’s milk by liquid chromatography–mass spectrometry analysis of β-lactoglobulin variants

Identification of Bioactive Peptides in Goat Milk and Their Health Application

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