The Potential of Prolonged Tissue Culture to Reduce Stress Generation and Retraction in Engineered Heart Valve Tissues

Vlimmeren, Marijke A. A. van; Driessen-Mol, Anita Anita; Oomens, C.W.J.; Baaijens, Fpt Frank

doi:10.1115/sbc2011-53120

Cited by 3 publications

(7 citation statements)

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“…The local realignment of the fibres during compaction leads to shortening of the scaffold in circumferential direction (as observed for samples of T = 28 days compared to T = 24 days) and an increase in structural stiffness (modulus) due to inhibition of local fibre realignment. The shortening of the scaffolds observed in the current study is slightly different from findings of van Vlimmeren et al 20 for tissue engineered heart valves. They reported that tissue compaction was initiated when the biodegradable polyglycolic acid/poly-4-hydroxybutyrate scaffolds lost their mechanical integrity.…”

Section: Ex Vivo Scaffold Mechanics Of Subcutaneous Implantscontrasting

confidence: 99%

Tissue ingrowth markedly reduces mechanical anisotropy and stiffness in fibre direction of highly aligned electrospun polyurethane scaffolds

Krynauw¹,

Buescher²,

Koehne³

et al. 2019

Preprint

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Purpose: The lack of long-term patency of synthetic vascular grafts currently available on the market has directed research towards improving the performance of small diameter grafts. Improved radial compliance matching and tissue ingrowth into the graft scaffold are amongst the main goals for an ideal vascular graft.Methods: Biostable polyurethane scaffolds were manufactured by electrospinning and implanted in subcutaneous and circulatory positions in the rat for 7, 14 and 28 days. Scaffold morphology, tissue ingrowth, and mechanical properties of the scaffolds were assessed before implantation and after retrieval.Results: Tissue ingrowth after 24 days was 96.5 ± 2.3% in the subcutaneous implants and 77.8 ± 5.4% in the circulatory implants. Over the 24 days implantation, the elastic modulus at 12% strain decreased by 59% in direction of the fibre alignment whereas it increased by 1379% transverse to the fibre alignment of the highly aligned scaffold of the subcutaneous implants. The lesser aligned scaffold of the circulatory graft implants exhibited an increase of the elastic modulus at 12% strain by 77% in circumferential direction.Conclusion: The mechanism underlying the softening of the highly aligned scaffold in the predominant fibre direction is thought to be associated with scaffold compaction and local displacement of fibres by the newly formed tissue. The stiffening of the scaffold, observed transverse to highly aligned fibres or for more a random fibre distribution, represents the actual mechanical contribution of the tissue.

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Section: Ex Vivo Scaffold Mechanics Of Subcutaneous Implantscontrasting

confidence: 99%

Tissue ingrowth markedly reduces mechanical anisotropy and stiffness in fibre direction of highly aligned electrospun polyurethane scaffolds

Krynauw¹,

Buescher²,

Koehne³

et al. 2019

Preprint

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show abstract

“…In this study, all materials were polymerised in the dogbone shape enabling the measurement of their tensile properties. Fibrin has previously been combined with polyglycolic acid, hyaluronic acidtyramine and collagen (van Vlimmeren et al 2013;Lee & Kurisawa 2013;Cummings et al 2004;Rowe et al 2007;Hokugo et al 2006) and while these combinations increased the mechanical properties of fibrin, the resistance to cell-mediated contraction remained minimal. This was due perhaps to the bonding between the materials.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is not necessarily the case that the stiffer the scaffold the better, as matrix stiffness also has the ability to regulate cell motility, proliferation and differentiation in various cell types (Discher et al 2005;Pho et al 2008;Yip et al 2009;Engler et al 2006;Haugh et al 2011). Additionally, many of these studies (van Vlimmeren et al 2013;Lee & Kurisawa 2013;Cummings et al 2004;Rowe et al 2007;Hokugo et al 2006) did not report strong cell viability.…”

Section: Discussionmentioning

confidence: 99%

“…Fibrin promotes ECM synthesis in the cells that it houses while also retaining deposited ECM due to the gel-like nature of the biomaterial. Fibrin, collagen and GAGs together have proven to be a suitable environment for both cardiovascular (Flanagan et al 2006;van Vlimmeren et al 2013;Alfonso et al 2013;Neidert & Tranquillo 2006) and cartilage applications (Eyrich et al 2007;Deponti et al 2014). GAGs such as chondroitin sulphate and hyaluronic acid are found abundantly in the cardiac cushion, from which valvulogenesis occurs, and studies have shown that the signalling molecules contained within these GAGs, regulate further heart valve development (Armstrong & Bischoff 2004;Person et al 2005;Eckert et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…0.75% collagen w/v is a higher concentration than used for orthopaedic applications in our lab (Tierney et al 2009;Keogh et al 2010), however, once infiltrated with fibrin, other concentrations did not have the inherent structural stability, even when crosslinked, to endure handling and manipulation when infiltrated with fibrin during experiments. Thirdly, GAGs have also been shown to reduce cell-mediated contraction (van Vlimmeren et al 2013). In studies where minimising contraction of fibrin was examined, the amount of GAGs available to the tissue had a direct impact on the level of cell contraction that occurred, with greater amounts of GAG leading to lower cell contraction.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Incorporation of fibrin into a collagen–glycosaminoglycan matrix results in a scaffold with improved mechanical properties and enhanced capacity to resist cell-mediated contraction

et al. 2015

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Fibrin is a versatile scaffold for tissue engineering applications, but its weak mechanical properties leave it susceptible to cell-mediated contraction, meaning the dimensions of the fibrin construct will change over time. We have reinforced fibrin with a collagen glycosaminoglycan matrix and characterised the mechanical properties and bioactivity of the reinforced fibrin (FCG). This is the first scaffold manufactured from all naturally derived materials that resists cell-mediated contraction. In fact, over 7 days, the FCG scaffold fully resisted cell-mediated contraction of vascular smooth muscle cells. This FCG scaffold has many potential applications where natural scaffold materials can encourage regeneration.

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Tissue Ingrowth Markedly Reduces Mechanical Anisotropy and Stiffness in Fibre Direction of Highly Aligned Electrospun Polyurethane Scaffolds

Krynauw

Buescher

Koehne

et al. 2020

Cardiovasc Eng Tech

View full text Add to dashboard Cite

Purpose: The lack of long-term patency of synthetic vascular grafts currently available on the market has directed research towards improving the performance of small diameter grafts. Improved radial compliance matching and tissue ingrowth into the graft scaffold are amongst the main goals for an ideal vascular graft.Methods: Biostable polyurethane scaffolds were manufactured by electrospinning and implanted in subcutaneous and circulatory positions in the rat for 7, 14 and 28 days. Scaffold morphology, tissue ingrowth, and mechanical properties of the scaffolds were assessed before implantation and after retrieval.Results: Tissue ingrowth after 24 days was 96.5 ± 2.3% in the subcutaneous implants and 77.8 ± 5.4% in the circulatory implants. Over the 24 days implantation, the elastic modulus at 12% strain decreased by 59% in direction of the fibre alignment whereas it increased by 1379% transverse to the fibre alignment of the highly aligned scaffold of the subcutaneous implants. The lesser aligned scaffold of the circulatory graft implants exhibited an increase of the elastic modulus at 12% strain by 77% in circumferential direction. Conclusion:Based on the observations, it is proposed that the mechanism underlying the softening of the highly aligned scaffold in the predominant fibre direction is associated with scaffold compaction and local displacement of fibres by the newly formed tissue. The stiffening of the scaffold, observed transverse to highly aligned fibres and for more a random fibre distribution, represents the actual mechanical contribution of the tissue that developed in the scaffold.

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The Potential of Prolonged Tissue Culture to Reduce Stress Generation and Retraction in Engineered Heart Valve Tissues

Cited by 3 publications

References 0 publications

Tissue ingrowth markedly reduces mechanical anisotropy and stiffness in fibre direction of highly aligned electrospun polyurethane scaffolds

Tissue ingrowth markedly reduces mechanical anisotropy and stiffness in fibre direction of highly aligned electrospun polyurethane scaffolds

Incorporation of fibrin into a collagen–glycosaminoglycan matrix results in a scaffold with improved mechanical properties and enhanced capacity to resist cell-mediated contraction

Tissue Ingrowth Markedly Reduces Mechanical Anisotropy and Stiffness in Fibre Direction of Highly Aligned Electrospun Polyurethane Scaffolds

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