The Predictive Ability of a CK5/p63/CK8/18 Antibody Cocktail in Stratifying Breast Papillary Lesions on Needle Biopsy

Reisenbichler, Emily; Adams, Amy L.; Hameed, Omar

doi:10.1309/ajcpxv64gxlzciga

Cited by 9 publications

(3 citation statements)

References 26 publications

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“…The present study has determined that myoepithelial markers (SMA & p63) associated with CK14 exhibit significantly higher expression rates in benign lesions in comparison to malignant lesions as evidenced by (P < .00), with high specificity (100%) and low sensitivity (63.3%). It is suggested that combined panel of P63, SMA, and CK14 may be utilized as reliable markers for distinguishing between benign and malignant papillary breast lesions.in agreement with previous work (29) which concluded that CK5/p63/CK8/18 antibody cocktail is a useful adjunct to morphology for evaluating breast papillary lesions. Chromogranin (CG) is of commonly used neuroendocrine markers, it was found to be negatively expressed in papilloma but highly positive in solid papillary carcinoma with more specificity 95% and less sensitivity 29% respectively.…”

Section: Discussionsupporting

confidence: 87%

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Bassyoni,

Elshawarby,

Abostate

2023

Benha Medical Journal

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Background: Papillary breast neoplasms are heterogenous group of breast diseases consisting of wide range of benign, atypical and malignant lesions. Evaluation of such papillary neoplasms is considered one of the most problematic issues in diagnosis of breast pathology depending on H&E only as they need different interventional treatment modalities. Aim of study: To differentiate papillary breast lesions depending on immunohistochemical staining of P63, SMA, CK14, chromogranin and ER. Materials and Methods: An Immunohistochemical study of P63, SMA, CK14, chromogranin and ER were conducted on 75 cases of different papillary breast neoplasms; papilloma (20 cases ) , DCIS ( 25 cases ) , solid papillary carcinoma ( 12 cases ), intracystic papillary carcinoma ( 11cases) and invasive micropapillary carcinoma (7 cases) . Results: Out of 75 cases, all cases of duct papillomas were positive to SMA, P63. Ninety percent (90 %) of them were positive to CK14 in comparison to malignant papillary: DCIS, intracystic papillary carcinomas. solid papillary carcinomas neoplasms which were negative (84.0%, 92.3 %, 90.0% & 100.0%, respectively). Ninety -two percentage (92 %) of DCIS cases, 92.3% intracystic papillary carcinomas, 90.0% solid papillary carcinomas and all cases of invasive micropapillary carcinomas-showed homogenous positivity for ER compared to papillomas that showed focal heterogenous expression with statistical significant (p<0.01). Solid papillary carcinoma cases were diffusely positive for chromgranin. P63 was more specific than SMA and CK14 in diagnosis of ductal papilloma (100% Specificity). chromogranin was more specific in discriminating solid papillary carcinoma (95.0% Specificity) .ER was the most sensitive marker in diagnosis of malignant papillary lesions (92.7 % sensitivity). Conclusion: A combined panel of markers consisting of P63, SMA, CK14, chromogranin and ER-could be helpful in differentiating begin, atypical and malignant papillary breast lesions which need different interventional treatment modalities. A wider scale study on different variants of malignant papillary lesions is recommended.

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Section: Discussionsupporting

confidence: 87%

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Bassyoni,

Elshawarby,

Abostate

2023

Benha Medical Journal

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“…P63 and Ki67 data presented in this study reinforced the statements of a large part of the authors, who affirm that the use of isolated markers is not sufficiently selective to separate benign from malignant and atypical lesions [10][11][12] .…”

Section: Discussionsupporting

confidence: 84%

“…Several markers can be used for breast tumors. In this work we used p63, a nuclear protein expressed in the basal epithelium of several organs, which in the breast has its positivity close to 100%, when the extracellular matrix is normal and in benign proliferative lesions; and Ki67, a protein whose function is associated with cell replication, staining nuclei at any stage of the cell cycle except G0 [7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of immunocytochemistry on the diagnosis of papillary mammary tumors by fine-needle aspiration biopsy

Mansani¹,

Bertucci²,

Netto³

et al. 2019

Mastology

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Este estudo observou a efetividade do método imunocitoquímico no diagnóstico das lesões papilares de mama a partir de amostras por punção aspirativa por agulha fina (PAAF), para validar o método que, aplicado à prática clínica, evitaria a exérese desnecessária dos pequenos papilomas intraductais da mama. Métodos: Foram analisados prontuários e exames de PAAF durante o período de 2003 a 2012 e posteriormente realizado exame imunocitoquímico com marcador de célula mioepitelial p63 e índice proliferativo Ki67, analisando-se especificidade e sensibilidade dos marcadores no diagnóstico de lesões papilares da mama. Resultados: A imunocitoquímica dos materiais das lesões papilares mamárias com os imunomarcadores Ki67 e p63 apresentou sensibilidade de 78,6% e especificidade de 73,33% na identificação das lesões benignas. Conclusões: O uso combinado desses marcadores em PAAF de lesão papilar mamária auxilia na orientação terapêutica da doença, mas novos estudos incluindo um maior número de casos devem ser realizados para melhor avaliar esse método.

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Applications of Rapid Immunohistochemistry on Frozen Tissue Sections During Intraoperative Pathological Diagnoses

Bailey,

Zhao

2022

Handbook of Practical Immunohistochemistry

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The Predictive Ability of a CK5/p63/CK8/18 Antibody Cocktail in Stratifying Breast Papillary Lesions on Needle Biopsy

Abstract: Although a single stain or combination cannot independently stratify papillary lesions, a CK5/p63/CK8/18 antibody cocktail is a useful adjunct to morphology for evaluating breast papillary lesions in needle biopsy specimens.

Cited by 9 publications

References 26 publications

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Diagnostic Significance Of Immunohistochemistry in Papillary Breast Neoplasms

Evaluation of immunocytochemistry on the diagnosis of papillary mammary tumors by fine-needle aspiration biopsy

Applications of Rapid Immunohistochemistry on Frozen Tissue Sections During Intraoperative Pathological Diagnoses

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