The Preparation and Structure Analysis Methods of Natural Polysaccharides of Plants and Fungi: A Review of Recent Development

Ren, Yimin; Bai, Yueping; Zhang, Zhidan; Cai, Wenlong; Flores, Antonio Del Rio

doi:10.3390/molecules24173122

Cited by 154 publications

(91 citation statements)

References 128 publications

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“…Natural polysaccharides ( Figure 13) are biocompatible, biodegradable, renewable linear or branched polymers consisting of different glucose units crosslinked by glycoside bonds [164,165]. These natural biomolecules have shown remarkable pharmaceutical activities such as anti-cancer activity [166], antiviral property [167] anti-inflammation activity [168] cardiovascular protection, and anti-mutation effects [169].…”

Section: Cyclodextrin Conjugate To Polysaccharides For Drug Deliverymentioning

confidence: 99%

Biocompatible Polymers Combined with Cyclodextrins: Fascinating Materials for Drug Delivery Applications

et al. 2020

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Cyclodextrins (CD) are a group of cyclic oligosaccharides with a cavity/specific structure that enables to form inclusion complexes (IC) with a variety of molecules through non-covalent host-guest interactions. By an elegant combination of CD with biocompatible, synthetic and natural polymers, different types of universal drug delivery systems with dynamic/reversible properties have been generated. This review presents the design of nano- and micro-carriers, hydrogels, and fibres based on the polymer/CD supramolecular systems highlighting their possible biomedical applications. Application of the most prominent hydrophobic aliphatic polyesters that exhibit biodegradability, represented by polylactide and polycaprolactone, is described first. Subsequently, particular attention is focused on materials obtained from hydrophilic polyethylene oxide. Moreover, examples are also presented for grafting of CD on polysaccharides. In summary, we show the application of host-guest interactions in multi-component functional biomaterials for controlled drug delivery.

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Section: Cyclodextrin Conjugate To Polysaccharides For Drug Deliverymentioning

confidence: 99%

Biocompatible Polymers Combined with Cyclodextrins: Fascinating Materials for Drug Delivery Applications

et al. 2020

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“…Weak anion-exchangers, such as diethylaminoethyl (DEAE), tertiary amine-functionalized media [93,94] or strong anion-exchangers including quaternary (Q) amine-functionalized media [95], have been successfully utilized to purify polysaccharide sulfates. SEC is extensively employed for the measurement of total molecular masses and molecular mass distributions [96][97][98][99][100]. Moreover, this analytical technique has been effectively employed in many examples for the elucidation of fine-structures of oligosaccharides and polysaccharides [94] and to produce size classes for the determination of structure-activity relationship [101][102][103].…”

Section: Purificationmentioning

confidence: 99%

Section: Purificationmentioning

confidence: 99%

“…Traditional methods for the determination of sulfate contents in polysaccharides include the HPLC analysis of In specific terms under standard conditions, the analysis of the structure of a given polysaccharide begins with its hydrolysis into constituent monosaccharides. Subsequently, the liberated monosaccharides are then analyzed by approved methods such as gas chromatography-mass spectroscopy (GC-MS) or high-performance liquid chromatography (HPLC) [96,[109][110][111]. The glycosidic linkages within polysaccharides are normally determined by methylation analysis with the classical Hakomori method or a modification thereof [112][113][114][115][116][117][118].…”

Section: Techniques For Structural Characterization Of Polysaccharidesmentioning

confidence: 99%

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Exploiting the Amazing Diversity of Natural Source-Derived Polysaccharides: Modern Procedures of Isolation, Engineering, and Optimization of Antiviral Activities

et al. 2020

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Naturally occurring polysaccharide sulfates are highly diverse, owning variations in the backbone structure, linkage pattern and stereochemistry, branching diversity, sulfate content and positions of sulfate group(s). These structural characteristics bring about diverse sulfated polymers with dissimilar negative charge densities and structure–activity relationships. Herein, we start with a short discussion of techniques needed for extraction, purification, chemical sulfation, and structural characterization of polysaccharides. Processes of isolation and sulfation of plant-derived polysaccharides are challenging and usually involve two steps. In this context, we describe an integrated extraction-sulfation procedure that produces polysaccharide sulfates from natural products in one step, thereby generating additional pharmacological activities. Finally, we provide examples of the spectrum of natural source-derived polysaccharides possessing specific features of bioactivity, in particular focusing on current aspects of antiviral drug development and drug–target interaction. Thus, the review presents a detailed view on chemically engineered polysaccharides, especially sulfated derivatives, and underlines their promising biomedical perspectives.

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“…Natural polysaccharides are a types of polymers comprising monosaccharide residues connected by glycosidic bonds, which derive from natural resources such as plants, animals, bacteria, fungi, algae, and so on. [1] It is well-known that mushroom-derived polysaccharides can inhibit cancer growth, and which usually was used as immunomodulators or biological response modifiers. [2] Fungal polysaccharide have shown significant effect against several human cancers, including lentinan, [3] Poriacocos polysaccharides, [4] Grifola frondosa polysaccharides, [5] there is no report on the study of polysaccharides from Inonotus cuticularis.…”

Section: Introductionmentioning

confidence: 99%

Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities

Guo

et al. 2020

Chemistry & Biodiversity

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Medical fungi polysaccharides belong to a very important species of biological macromolecules, which are the basic substances that effectively maintain and ensure the normal operation of biological life activities. However, research on extraction and biological activity of Inonotus cuticularis polysaccharides has never been reported. In this study, the optimum yield of Inonotus cuticularis polysaccharides was determined by the orthogonal experimental design. The highest yield of 3.10±0.06 % was obtained with extraction temperature of 80 °C, extraction time of 150 min, and water to raw material ratio of 30 mL/g and repeated twice. After deproteinization for 5 times, the protein removal rate reached 70.10±1.75 %, and the content of polysaccharides and protein were 46.64 and 0.42 %. Infrared spectrometer indicated that Inonotus cuticularis polysaccharides are typical β‐pyranose with characteristic peaks of polysaccharides. Subsequently, the activities of scavenging free radicals for the deproteinated polysaccharides were studied. When the concentration of Inonotus cuticularis polysaccharides was 0.3 mg/mL, the scavenging activities of the sample on DPPH., .OH, ABTS.+ and O2.− reached 83.67±0.27, 65.21±4.82, 43.45±1.36 and 80.28±2.30 %, respectively, and the reducing power reached 0.46±0.01. The IC50 values scavenging DPPH., .OH, ABTS.+ and O2.− were 0.139±0.13, 0.162±0.14, 0.317±0.30 and 0.121±0.10 mg/mL, respectively. Results showed that Inonotus cuticularis polysaccharides present potential stronger antioxidant activities, especially .OH scavenging activity and reducing power. Experimental results could provide research basis of Inonotus cuticularis polysaccharides for further exploitation and utilization.

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The Preparation and Structure Analysis Methods of Natural Polysaccharides of Plants and Fungi: A Review of Recent Development

Cited by 154 publications

References 128 publications

Biocompatible Polymers Combined with Cyclodextrins: Fascinating Materials for Drug Delivery Applications

Biocompatible Polymers Combined with Cyclodextrins: Fascinating Materials for Drug Delivery Applications

Exploiting the Amazing Diversity of Natural Source-Derived Polysaccharides: Modern Procedures of Isolation, Engineering, and Optimization of Antiviral Activities

Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities

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