The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA

Abstract

Molecularly imprinted gel polymer (MIP) for the selective imprinting of bovine hemoglobin (BHb) was prepared in aqueous media by bulk polymerization using polyacrylamide matrix. The synthesis conditions of BHb-MIP were investigated, which involved the interaction of functional monomers and template protein in different molar ratio, solution pH, and ionic strength. The adsorption experiments indicated that BHb-MIP had a high affinity for BHb over the non-imprinted polymers. The selectivity of BHb-MIP for BHb and bovine serum albumin (BSA) with similar molecular weight was compared. It was demonstrated that BHb-MIP had better selective adsorption and recognition properties to BHb especially in the presence of BSA as competing protein. It might be helpful for selectively separating template protein with MIP from the proteins mixture with similar molecular weight.

The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation

“…The amount of BSA eluted from In terms of the imprinting factor (˛) in Table 2, when the concentrations of competing proteins Lyz and Mb kept constant in competitive adsorption experiments, higher concentration template BSA made its corresponding˛increasing from 1.43 to 2.11, but values of the two competing proteins Lyz and Mb decreasing from 0.81 to 0.56 and 1.35 to 1.00. The result is a good complementary to that conclusion "when the concentrations of template proteins kept unchanged and the amount of competing protein increased, their imprinting factor were primarily invariable" [31].…”

MIP-based electrochemical protein profiling

“…The four proteins chosen were on the basis of their different biological roles, sizes, and electrochemical activities. Bovine hemoglobin (BHb, 64.5 kda) well known for its function in the vascular system as a carrier of oxygen, also in aiding the transport of carbon dioxide and regulating blood pH [26]. Both BHb and EMb (17.5 kda) exhibit well-known electrochemical behaviour [1,24,27,28].…”

Advances in the development of molecularly imprinted polymers for the separation and analysis of proteins with liquid chromatography

“…A brief synopsis of the different types of protein and biomacromolecular surface‐imprinted monoliths and particles/beads is shown in Tables and , respectively, including the various surface imprinting strategies and characterization techniques that have been utilized.…”