The Prevalence of Pathogenic Periodontal Microflora in Healthy Young Adult Smokers

Shiloah, Jacob; Patters, Mark R.; Waring, Margaret B.

doi:10.1902/jop.2000.71.4.562

Cited by 58 publications

(62 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The present study found no correlation between smoking and the prevalence of the five tested bacteria. It is true that Shiloah et al (27) showed that the prevalence of pathogenic bacteria in the periodontal sulcus of periodontitis-free individuals is related to their daily consumption of cigarettes and duration of cigarette smoking. However, it is not realistic to monitor the daily consumption of cigarettes by younger smokers, even if this factor may affect the early colonization by periodontal pathogens in the periodontal sulcus of those in the younger populations.…”

Section: Vol 46 2008 Age For Initial Colonization Of Periodontal Pamentioning

confidence: 99%

Etiological Analysis of Initial Colonization of Periodontal Pathogens in Oral Cavity

et al. 2008

View full text Add to dashboard Cite

It is unclear when the initial colonization by periodontal pathogens occurs in the oral cavity. Therefore, we report here the association between specific age groups and the time when the initial colonization by periodontal pathogens occurs in the oral cavity in such groups. Findings are based on an epidemiological analysis of the prevalence of five periodontal pathogens in the oral cavities of a wide range of age populations, from newborn to elderly, who were randomly selected in a geographic region of Brazil. These periodontal pathogens include Campylobacter rectus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Tannerella forsythia and were analyzed in the bacterial samples isolated from gingival sulcus, the dorsum of the tongue, and cheek mucosa of diverse age groups, using a bacterial DNA-specific PCR method. Results indicated that there are distinct age-related groups where initial colonization by the five periodontal pathogens examined in this study can be detected and that the presence of teeth is a permissive factor for colonization by P. gingivalis, P. intermedia, and T. forsythia. Although it remains unclear exactly how or when target pathogens colonize healthy subjects, an understanding of age-related groups does provide a potentially useful tool in the early detection and prevention of periodontitis in healthy individuals.

show abstract

Section: Vol 46 2008 Age For Initial Colonization Of Periodontal Pamentioning

confidence: 99%

Etiological Analysis of Initial Colonization of Periodontal Pathogens in Oral Cavity

et al. 2008

View full text Add to dashboard Cite

show abstract

“…These irritants cause white lesions. Smoking can also cause abnormalities in the oral cavity such as the tongue, gums, mouth mucosa, teeth and palate in the form of nicotine stomatitis and fungal infections [19,20]. All of these factors can cause DNA damage [21,22].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Quality of DNA Extracted from Buccal Swabs for Microarray Based Genotyping

Alex¹,

Lye²,

Jagdish³

et al. 2011

Ind J Clin Biochem

View full text Add to dashboard Cite

Buccal cell usage has been shown by many to be a cost effective and safe method to isolate DNA for various biological experiments especially large epidemiological studies (Garcia-Closas et al. Cancer Epidemiol Biomarkers Prev 10:687-696, 2001). Non-invasive DNA collection methods are preferred over phlebotomy in order to increase study participation and compliance in research centers and for sick patients in hospital settings. There have been conflicting reports about the methodology and results obtained from using buccal DNA. It is not very clear if phlebotomy can be confidently replaced by buccal cell DNA. It is often left for the user to take an intelligent decision. To address this issue, we compared the performance of buccal and blood DNA from same subjects in a genotyping experiment and this paper reports the results. Cotton swab derived buccal cells were scraped from the inner side of cheeks from 16 subjects, and blood was also drawn from the same 16 subjects participating in a genotypic association study of a lipid disease. The DNA quality was assessed by resolving on agarose gels, checking purity (A260/A280) and finally by microarray hybridization. This study showed that DNA degradation affects the total yield and performance of the buccal DNA when compared to the blood DNA in microarray based genotyping. Genotyping results can be seriously compromised if care is not taken to check the quality and yields of such specimens.

show abstract

“…Tobacco smoke has been shown to cause shifts in the microbial species that comprise dental plaque (Haffajee & Socransky 2001, Kamma et al 1999, Shiloah et al 2000, Umeda et al 1998, van Winkelhoff et al 2001, Zambon et al 1996. In a recent study by our group , it was hypothesized that tobacco may induce alterations to the molecular structure of lipid A in a manner consistent with reduced inflammatory potential.…”

Section: Introductionmentioning

confidence: 99%

Effects of Tobacco Smoking on Chronic Periodontitis and Periodontal Treatment

Buduneli¹

2012

Pathogenesis and Treatment of Periodontitis

View full text Add to dashboard Cite

The Prevalence of Pathogenic Periodontal Microflora in Healthy Young Adult Smokers

Abstract: These data indicate that the prevalence of colonization of the sulcus by pathogenic bacterial species in periodontitis-free individuals is related to the quantity and duration of cigarette smoking.

Cited by 58 publications

References 42 publications

Etiological Analysis of Initial Colonization of Periodontal Pathogens in Oral Cavity

Etiological Analysis of Initial Colonization of Periodontal Pathogens in Oral Cavity

Evaluation of Quality of DNA Extracted from Buccal Swabs for Microarray Based Genotyping

Effects of Tobacco Smoking on Chronic Periodontitis and Periodontal Treatment

Contact Info

Product

Resources

About