The Probiotic Lactobacillus Prevents Citrobacter rodentium-Induced Murine Colitis in a TLR2-Dependent Manner

Ryu, Seunghyun; Park, Jong‐Hyung; Choi, Seri; Jeon, Hee‐Yeon; Park, Jin‐Il; Kim, Junyoung; Ham, Seung‐Hoon; Choi, Yang‐Kyu

doi:10.4014/jmb.1602.02004

Cited by 17 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C. rodentium-infected mice develop various pathologies depending on their age, genetic background, diet, and indigenous microbiota (Mundy et al, 2005). C. rodentium infection in the suckling and immunodeficient mice results in the development of colitis, characterized by diarrhea, rectal prolapse, and other clinical signs including retarded growth, listlessness, dehydration, and mortality (in severe cases) (Luperchio and Schauer, 2001;Ryu et al, 2016). In contrast, the infection in adult mice is subclinical and self-limiting, resulting in considerably less morbidity or mortality (Maaser et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium

et al. 2018

Self Cite

View full text Add to dashboard Cite

Adult mice were treated with dextran sulfate sodium (DSS) and infected with Citrobacter rodentium for developing a novel murine colitis model. C57BL/6N mice (7-week-old) were divided into four groups. Each group composed of control, dextran sodium sulfate-treated (DSS), C. rodentium-infected (CT), and DSS-treated and C. rodentium-infected (DSS-CT) mice. The DSS group was administered 1% DSS in drinking water for 7 days. The CT group was supplied with normal drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The DSS-CT group was supplied with 1% DSS in drinking water for 7 days and subsequently infected with C. rodentium via oral gavage. The mice were sacrificed 10 days after the induction of C. rodentium infection. The DSS-CT group displayed significantly shorter colon length, higher spleen to body weight ratio, and higher histopathological score compared to the other three groups. The mRNA expression levels of tumor necrosis factor (TNF)-α and interferon (INF)-γ were significantly upregulated; however, those of interleukin (IL)-6 and IL-10 were significantly downregulated in the DSS-CT group than in the control group. These results demonstrated that a combination of low DSS concentration (1%) and C. rodentium infection could effectively induce inflammatory bowel disease (IBD) in mice. This may potentially be used as a novel IBD model, in which colitis is induced in mice by the combination of a chemical and a pathogen.

show abstract

Section: Introductionmentioning

confidence: 99%

A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Probiotic bacteria and Lactobacillus bacteria in particular have previously been shown to induce their beneficial effects through toll-like receptor (TLR) signaling, specifically TLR2 45,46 . Furthermore, bacteria are able to induce a signaling cascade through activation of the Nucleotide-binding Oligomerization Domain (NOD) pathway 47 .…”

Section: Resultsmentioning

confidence: 99%

“…Of the cytosolic PRRs, the nucleotide binding and oligomerization domain (NOD)-like receptor family members NOD1 and NOD2 are the most well understood 64 . Studies have suggested that Lactobacillus species potentially interacts with the immune system and induces a response through TLR-2 45,62,65 , TLR-4 and DC-specific intracellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) 66 . Furthermore, studies utilizing NOD1 and NOD2 knockout mice have identified that these receptors play a key role in the regulation of bone mass by the intestinal microbiota 67 .…”

Section: Discussionmentioning

confidence: 99%

Beneficial effects of Lactobacillus reuteri 6475 on bone density in male mice is dependent on lymphocytes

Collins

Rios‐Arce

Schepper

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Oral treatment with probiotic bacteria has been shown to prevent bone loss in multiple models of osteoporosis. In previous studies we demonstrated that oral administration of Lactobacillus reuteri in healthy male mice increases bone density. The host and bacterial mechanisms of these effects however are not well understood. The objective of this study was to understand the role of lymphocytes in mediating the beneficial effects of L. reuteri on bone health in male mice. We administered L. reuteri in drinking water for 4 weeks to wild type or Rag knockout (lack mature T and B lymphocytes) male mice. While L. reuteri treatment increased bone density in wild type, no significant increases were seen in Rag knockout mice, suggesting that lymphocytes are critical for mediating the beneficial effects of L. reuteri on bone density. To understand the effect of L. reuteri on lymphocytes in the intestinal tissues, we isolated mesenteric lymph node (MLN) from naïve wild type mice. In ex vivo studies using whole mesenteric lymph node (MLN) as well as CD3+ T-cells, we demonstrate that live L. reuteri and its secreted factors have concentration-dependent effects on the expression of cytokines, including anti-inflammatory cytokine IL-10. Fractionation studies identified that the active component of L. reuteri is likely water soluble and small in size (<3 kDa) and its effects on lymphocytes are negatively regulated by a RIP2 inhibitor, suggesting a role for NOD signaling. Finally, we show that T-cells from MLNs treated with L. reuteri supernatants, secrete factors that enhance osterix (transcription factor involved in osteoblast differentiation) expression in MC3T3-E1 osteoblasts. Together, these data suggest that L. reuteri secreted factors regulate T-lymphocytes which play an important role in mediating the beneficial effects of L. reuteri on bone density.

show abstract

“…Several reports have shown that the immunostimulant effect of the organism, in response to probiotics, is dependent in part on activation of Toll-like receptors (TLRs) [15,16]. Monocytes and macrophages express high levels of TLRs, mainly TLR2, TLR4, and TLR6, which have been demonstrated to induce cytokine and chemokine production upon stimulation by several probiotic species and strains [17][18][19]. Macrophages also participate in the activation and regulation of the immune response through antitumor activities, antigen presentation, and secretion of both pro-inflammatory cytokines (e.g., TNF-α, IL-1, and IL-6) and anti-inflammatory cytokines (IL-10 and transforming growth factor (TGF)-β) that act to regulate immune homeostasis [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro

et al. 2020

View full text Add to dashboard Cite

Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-β) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain.

show abstract

The Probiotic Lactobacillus Prevents Citrobacter rodentium-Induced Murine Colitis in a TLR2-Dependent Manner

Cited by 17 publications

References 0 publications

A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium

A murine colitis model developed using a combination of dextran sulfate sodium and Citrobacter rodentium

Beneficial effects of Lactobacillus reuteri 6475 on bone density in male mice is dependent on lymphocytes

Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro

Contact Info

Product

Resources

About