The Production of Artemisinin in Artemisia annua L. Tissue Cultures

Martínez, Benito Arias; Staba, E. John

doi:10.1016/b978-0-12-007906-3.50009-7

Cited by 54 publications

(48 citation statements)

References 41 publications

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“…These results are an indication that differentiated shoot cultures could serve as high-value products for pharmaceutical use, since they contain artemisinin levels comparable with those observed during agricultural production. However, the low biomass produced makes it definite that tissue cultures might not be a suitable strategy for Artemisinin is produced by differentiated (shoots and roots) shoot cultures 12,13 however, occurs only in shoots without roots, 9,11,[14][15][16] these reports are congruent to our observations where the artremisinin content was observed in in vitro shoots only. Brown 21 reported 0.0038% of artemisinin being produced by callus cultures bearing shoots but did not specify whether or not they had roots.…”

Section: Resultssupporting

confidence: 82%

“…A comparative evaluation of the artemisinin content was also studied in tissue culture, green house and field grown plants of Artemisia by Ferreira et al 7 Artemisinin was observed to be produced by differentiated shoot culture 11,12,13 but only at trace level. 9,14,15,16 Selection for high-producing lines through traditional breeding, and research on the effects of environmental conditions and cultural practices could perhaps lead to an improvement of artemisinin content, 7,17,18,19 as its maintenance in the natural population is difficult due to its open pollinated nature. Therefore, it exhibits a great range of variability in its artemisinin yield in field condition due to various environmental and agricultural factors.…”

Section: Introductionmentioning

confidence: 99%

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Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

Paul

Gupta

Khanuja

2012

Nat. Prod. Bioprospect.

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An efficient protocol for maintaining the artemisinin content in tissue culture and high frequency of in vitro direct and indirect regenerations of multiple shoots of high artemisinin yielding genotypes of Artemisia annua has been developed and their comparison with field grown mother plant has been carried out. The eleven elite genotypes (containing more than 1% artemisinin) were tested on seven different modified media formulations. Modified half MS (Murashige and Skoog's) media containing 100 mg L -1 myo-inositol, 0.5 g L -1 casine hydrolysate, 5 mg L -1 biotin, 2 mL L -1 RT (Revised Tobacco) vitamin stock, 0.5 mg L -1 BAP and 0.01 mg L -1 NAA showed best regeneration while, above modified MS medium containing 0.2 mg L -1 BAP and 0.2 mg L -1 NAA showed maximum shoot multiplication with maintained artemisinin content. Based on the chemical profiles of both the systems, minor difference was observed in their artemisinin content. A large scale culture of these plants maintained the normal growth index along with the artemisinin content and could be a better alternative to maintain the high artemisinin yielding genotypes with their genetic constraint in specific media combinations and also used as base material for further genetic improvement.

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Section: Resultssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

Paul

Gupta

Khanuja

2012

Nat. Prod. Bioprospect.

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“…Moreover, the content of artemisinin in such differentiated cultures was much lower than the whole plant (Martinez and Staba, 1988). Recently, wide variations in artemisinin content were observed among various accessions of A .…”

Section: Introductionmentioning

confidence: 99%

Selection and micropropagation of high artemisinin producing clones of Artemisia annua L.

Elhag

El-Domiaty

El‐Feraly

et al. 1992

Phytotherapy Research

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“…Artemisinin has been detected in leaves, small green stems, buds, flowers and seeds of A. annua (Acton et al, 1985;Zhao and Zeng, 1985;Liersch et al, 1986;Martinez and Staba, 1988;Singh et al, 1988;Madhusudanan, 1989;. Artemisinin has not been reported in roots of field-grown plants (Pras et al, 1991;Klayman, 1993; or pollen, and the detection of artemisinin from seeds appears to be due to the presence of floral debris because seeds have no glandular trichomes .…”

Section: Artemisinin and Artemisia Annuamentioning

confidence: 99%

Cultivation and genetics ofArtemisia annuaL. for increased production of the antimalarial artemisinin

Ferreira¹,

Laughlin²,

Delabays

et al. 2005

Plant Genet. Resour.

111

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Malaria has been treated for over 350 years with quinine and quinine-derived drugs. However, in several areas of the world, some strains of the malarial parasite Plasmodium falciparum have developed resistance against these drugs. Recently, the World Health Organization (WHO) recommended the use of artemisinin-combination treatments (ACT) as the first-line treatment for multidrug-resistant falciparum malaria. The WHO estimates that current supplies of artemisinin are sufficient for only 30 million ACT, and is foreseeing the need for 130-220 million ACT in 2005 (WHO, 2004. Current research on the production of synthetic artemisinin-like compounds by the Roll Back Malaria project, pharmaceutical companies and academia resulted in a promising synthetic artemisinin-like compound (OZ277) which is currently undergoing phase I clinical trials. In about 5 years this drug is expected to be approved and made available to the public, however, meeting current global demands for ACT depends on the immediate availability of affordable artemisinin-derived drugs. This will involve expansion of the area under cultivation of Artemisia annua and improved methods of cultivation and processing of raw material, associated with more efficient methods for extraction and purification of artemisinin from plant material. This review addresses the agricultural, environmental and genetic aspects that may be useful in the successful large-scale cultivation of A. annua and for producing the antimalarial artemisinin in areas where it is urgently needed today. It also includes geographic aspects (latitude and altitude), which will help make decisions about crop establishment in tropical countries, and includes a list of Good Agricultural and Collection Practices for A. annua.

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The Production of Artemisinin in Artemisia annua L. Tissue Cultures

Cited by 54 publications

References 41 publications

Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

Maintaining the artemisinin content through direct and indirect in vitro regeneration and their assessment of variations with the field grown mother plants of Artemisia annua L.

Selection and micropropagation of high artemisinin producing clones of Artemisia annua L.

Cultivation and genetics ofArtemisia annuaL. for increased production of the antimalarial artemisinin

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