The prognostic implications of microvascular density and lymphatic vessel density in esophageal squamous cell carcinoma: Comparative analysis between the traditional whole sections and the tissue microarray

Chen, Bo; Fang, Wang‐Kai; Wu, Zhi‐Yong; Xu, Xiu‐E; Wu, Jian‐Yi; Fu, Junhui; Yao, Xiaodong; Huang, Jianhao; Chen, Jiexin; Shen, Jian; Chen, Zheng; Wang, Shaohong; Li, En‐Min; Xu, Li‐Yan

doi:10.1016/j.acthis.2013.12.003

Cited by 17 publications

(12 citation statements)

References 31 publications

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“…Since microvascularization differs within the tumor, MVD is usually averaged from several or several dozen places throughout tumor cross-section, whereas the TMA technique allows for microvessel assessment only in its selected fragments. Thus, TMAs might not fully reflect the true degree of microvascularization in a tumor, as also suggested by others [21, 22]. …”

Section: Discussionmentioning

confidence: 91%

Microvessel density in head and neck squamous cell carcinoma

Szafarowski

Sierdziński

Szczepański

et al. 2018

Eur Arch Otorhinolaryngol

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PurposeMicrovessel density (MVD) corresponds to the intensity of neo-angiogenesis. MVD assessments are based on the expression levels of the vascular endothelium markers such as, e.g., CD34 or CD105. The goal of this study was to assess MVD among patients with head and neck squamous cell carcinoma (HNSCC), and to evaluate the predictive value of MVD in head and neck cancers.MethodsThe study included 49 patients treated for HNSCC and 11 patients with dysplasia of the upper respiratory tract epithelium. Control tissues consisted of 12 normal mucous membranes of the throat. Expression levels of MVD markers were assessed by immunohistochemistry (IHC) using tissue microarrays (TMA). Clinicopathological factors and patients’ survival over the 5-year follow-up period were analyzed.ResultsThe MVD/CD34 values were found to be significantly elevated in the HNSCCs compared to the non-malignant control tissues (p = 0.001) and to dysplastic tissues. (p = 0.02). Significantly higher MVD/CD105 values were also seen in the tumor compared to the control tissues (p = 0.001) or the dysplastic tissues (p = 0.001). Unexpectedly, significantly lower MVD/CD34 values were seen in the tumor tissues of patients with the T3–T4 tumors compared to those with T1–T2 tumors (p = 0.01).ConclusionsHNSCCs have statistically higher MVD values compared to dysplasia of the upper respiratory tract epithelium. However, the MVD/CD34 values did not correlate with local invasiveness (the T feature) of HNSCCs. This counterintuitive observation suggests that assessments of MVD as performed on TMA by IHC using anti-CD34 or anti-CD105 antibodies considered to be specific for endothelial cell markers might underestimate the extent of the tumor vascularity in HNSCC.

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Section: Discussionmentioning

confidence: 91%

Microvessel density in head and neck squamous cell carcinoma

Szafarowski

Sierdziński

Szczepański

et al. 2018

Eur Arch Otorhinolaryngol

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“…[52] Although software, such as CIAS (computer-aided image analysis system), was designed to mellow these bias, yet its accuracy needs to be further tested. [17,53] Second, the MVD was derived from a tissue section, which means that MVD could not indicate the whole in vivo condition or the dynamic tumoral status. Lastly, to date, debate continues on which antibody was most suitable for immunohistochemical staining in MVD assessment.…”

Section: Discussionmentioning

confidence: 99%

“…CD34 was a frequently used marker, but it failed to differentiate normal vessels and newly formed vessels. [15] Some believed that CD105 has superior specificity with newly generated endothelial cells, [53,54] yet few studies measured ESCC MVD through CD105, and evidence remained insufficient to draw a conclusion. Despite the flaws mentioned above, to date, MVD is still the most widely used method, and is considered as the golden standard to assess angiogenesis quantitatively.…”

Section: Discussionmentioning

confidence: 99%

Microvessel density as a prognostic factor in esophageal squamous cell cancer patients

Ma¹,

Zhang²,

Jiang

et al. 2017

Medicine

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Background:To date, literature has emerged that shows contradictory results about the prognostic role of microvessel density (MVD) in esophageal squamous cell cancer (ESCC). The aim of the study set out to evaluate the correlation between MVD and the prognosis of ESCC.Methods:Identified publications from various databases were obtained and reviewed. A meta-analysis was performed to evaluate the prognostic role of MVD among ESCC patients.Results:A total of 11 eligible studies containing 891 ESCC cases were included in the meta-analysis. The pooled hazard ratio for overall survival was 2.39 (95% confidence interval 1.92–2.96, P < .001). Heterogeneity among the studies was not significant, and publication bias was not found. Subgroup analyses were also performed on different issues, such as districts, antibodies, and median age.Conclusion:High MVD is a prognostic factor among ESCC that indicated worse prognosis in these patients. More studies are needed, and through abundant evidence, the topic could be re-evaluated by then.

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“…[7][8][9] Tumour angiogenesis is indispensable for tumour growth and metastasis, and has diagnostic, prognostic and therapeutic implications. [10][11][12] Raica et al classified tumour blood vessels in thymomas as immature, intermediate, and mature, and quantified endothelial cell proliferation; 13 they suggested that blood vessel type and endothelial cell proliferation were correlated with invasiveness. Tomita et al showed a correlation between tumour angiogenesis and invasiveness of thymomas.…”

Section: Introductionmentioning

confidence: 99%