The Protecting Effects and Mechanisms of Baicalin and Octreotide on Heart Injury in Rats with SAP

Zhang, Xiping; Tian, Hua; Chen, Hanqing; Li, Chen; Wang, Zhiwei; Wang, Keyi; Yan, Weiqun; Li, Yun; Li, Qingyu; He, Qing; Fei, Wang

doi:10.1155/2007/19469

Cited by 28 publications

(39 citation statements)

References 40 publications

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“…A previous report confirmed that baicalin could have a protective effect on heart injury in rats with severe acute pancreatitis. 22) Baicalin has also shown potent protection against ischemia/reperfusion injury in cultured chick cardiomyocytes. 23) In the cultured rat cardiomyocytes exposed to hypoxia/reoxygenation, baicalin treatment effectively inhibited the nuclear translocation of NF-κB and exerted cardioprotective efficacy.…”

Section: Effects Of Baicalin On the Caspase-3 Protein And Mapk Cascadmentioning

confidence: 99%

Baicalin Attenuates Acute Myocardial Infarction of Rats <i>via</i> Mediating the Mitogen-Activated Protein Kinase Pathway

Liu

Fan

Shi

et al. 2013

Biological & Pharmaceutical Bulletin

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Baicalin is a bioactive ingredient from the herb and has possessed various pharmacological actions. The present study was performed to evaluate the cardioprotective potential of baicalin against myocardial infarction and explore the potential mechanism. Baicalin was intraperitoneally injected into the rats by the doses of 50, 100 and 200 mg/kg, respectively, once a day for 7 d and, 30 min after the last administration, the left coronary artery was ligated. Infarct size was measured to analyze the myocardial damage. Myocardial specific enzymes, including creatine kinase (CK), the MB isoenzyme of creatine kinase (CK-MB), lactate dehydrogenase (LDH) and cardiac troponin T (cTnT) were determined with the colorimetric method. Evidence for myocardial apoptosis was detected by caspase-3 activity measurement and Western blot analysis. We also examined the protein levels of three major subgroups of mitogen-activated protein kinases (MAPKs), namely, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 by immuoblotting. Our results indicated that baicalin significantly reduced the infarct size and myocardial enzymes (CK, CK-MB, LDH and cTnT). Administration of baicalin also suppressed the activity and protein expression of caspase-3. Moreover, the protein level of phosphorylated ERK (p-ERK) was found to be evidently augmented while the phosphorylated JNK (p-JNK) and phosphorylated p38 (p-p38) were strikingly diminished in infarcted rats with baicalin treatment. These findings suggest that the baicalin's cardioprotection associates with mediation of MAPK cascades in acute myocardial infarction of rats.

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Section: Effects Of Baicalin On the Caspase-3 Protein And Mapk Cascadmentioning

confidence: 99%

Baicalin Attenuates Acute Myocardial Infarction of Rats <i>via</i> Mediating the Mitogen-Activated Protein Kinase Pathway

Liu

Fan

Shi

et al. 2013

Biological & Pharmaceutical Bulletin

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“…Experimental Medicine and Reagents [13][14][15][16] Sodium taurocholate and sodium pentobarbital obtained from Sigma Company (USA); Octreotide obtained from Novartis pharmaceutical company (Swiss); 5% Baicalin injection prepared by the first author (China national invention patent number ZL200310122673.6). The NF-κB p65 and P-Selectin antibody obtained from Santa Cruz Company (USA).…”

Section: Experimental Animalsmentioning

confidence: 99%

Influence of Baicalin and Octreotide on NF-κB and P-Selectin Expression in Liver and Kidney of Rats with Severe Acute Pancreatitis

Zhang

et al. 2008

Inflammation

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To observe the influence of Baicalin and Octreotide on liver and kidney of rats with severe acute pancreatitis (SAP) and discuss the related mechanism. SAP rats were randomly divided into model control, Baicalin treated and Octreotide treated group (n = 45). The same number of normal rats were included in sham-operated group (n = 45). In all groups, the mortality rate, pathological changes as well as expression levels of NF-kappaB p65 and P-selectin protein in liver and kidney were observed at 3, 6 and 12 h after operation. The survival rate of treated group was 100% at 12 h significantly higher than that of model control group (P < 0.05). The pathological changes of liver and kidney in treated groups were alleviated to different degrees, the NF-kappaB protein expression levels and pathological severity scores in liver and kidney of treated groups were significantly lower than those of model control group (P < 0.05 or P < 0.001). The hepatic P-selectin protein expression level in Baicalin treated group was significantly lower than that of model control group at 3 h (P < 0.01), and renal P-selectin expression level in Baicalin treated group at 3 and 6 h were significantly lower than those of model control group and Octreotide treated group (P < 0.01). (1) Early treatment with Baicalin or Octreotide have obvious protecting effects on liver and kidney injuries in SAP with their mechanisms associated to inhibiting NF-kappaB and P-selectin expression of liver and kidney. (2) Comparing the pharmacologic effects of Octreotide and Baicalin, we believe Baicalin as a new drug with its protecting effects on liver and kidney of SAP rats similar to Octreotide is worth further studying. (3) The advantages of tissue microarrays in pathological examination include time and energy saving and highly efficient. But the restriction of small diameter weakens the representation of tissues to various extents, which may lead to the deviation of analysis.

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“…Several groups reported that baicalin induced apoptosis of cancer cells [46] and carcinoma cells [47], via ERK and p38 MAPK signaling pathways. However, baicalin also showed cytoprotective effects on CMs [48,49], hepatocytes and neural cells [50,51,52]. A latest study reported that baicalin attenuates acute myocardial infarction of rats via mediating ERK and p38 MAPK signaling pathways [48].…”

Section: Discussionmentioning

confidence: 99%

Baicalin Maintains Late-Stage Functional Cardiomyocytes in Embryoid Bodies Derived from Murine Embryonic Stem Cells

Tang

Yin

Tang

et al. 2013

Cell Physiol Biochem

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Background/Aims: Low efficiency of cardiomyocyte (CM) differentiation from embryonic stem (ES) cells limits their therapeutic use. The objective of this study was to investigate the effect of baicalin, a natural flavonoid compound, on the in vitro cardiac differentiation of murine ES cells. Methods: The induction of ES cells into cardiac-like cells was performed by embryoid body (EB)-based differentiation method. The electrophysiological properties of the ES cell-derived CMs (ES-CMs) were measured by patch-clamp. The biomarkers of ES-CMs were determined by quantitative RT-PCR and immunofluorescence. Results: Continuous baicalin treatment decreased the size of EBs, and increased the proportion of α-actinin-positive CMs and transcript level of cardiac specific markers in beating EBs by inducing cell death of non-CMs. Baicalin increased the percentage of working ES-CMs which had typical responses to β-adrenergic and muscarinic stimulations. Conclusion: Baicalin maintains the late-stage functional CMs in EBs derived from murine ES cells. This study describes a new insight into the various biological effects of baicalin on cardiac differentiation of pluripotent stem cells.

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The Protecting Effects and Mechanisms of Baicalin and Octreotide on Heart Injury in Rats with SAP

Cited by 28 publications

References 40 publications

Baicalin Attenuates Acute Myocardial Infarction of Rats <i>via</i> Mediating the Mitogen-Activated Protein Kinase Pathway

Baicalin Attenuates Acute Myocardial Infarction of Rats <i>via</i> Mediating the Mitogen-Activated Protein Kinase Pathway

Influence of Baicalin and Octreotide on NF-κB and P-Selectin Expression in Liver and Kidney of Rats with Severe Acute Pancreatitis

Baicalin Maintains Late-Stage Functional Cardiomyocytes in Embryoid Bodies Derived from Murine Embryonic Stem Cells

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