The protection of selenium on ROS mediated-apoptosis by mitochondria dysfunction in cadmium-induced LLC-PK1 cells

Zhou, Yijing; Zhang, Shiping; Liu, Chang-Wei; Cai, Yuanqing

doi:10.1016/j.tiv.2008.12.009

Cited by 171 publications

(105 citation statements)

References 31 publications

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“…However, high levels of ROS in the mitochondria may cause mitochondrial membrane depolarization, the release of mitochondrial factors and triggering of caspase cascades (18). Under normal conditions, the majority of anticancer drugs target mitochondrial function, producing ROS (19)(20)(21)(22). Therefore, ROS is important in mitochondria-mediated apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Alantolactone induces apoptosis in RKO cells through the generation of reactive oxygen species and the mitochondrial pathway

Zhang

Bao

Wu³

et al. 2013

Molecular Medicine Reports

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Abstract. Alantolactone, a methanol extract of Inula helenium, possesses anticancer properties in a number of cancer cell lines. However, its anticancer effect on human colorectal cancer cells and the underlying mechanisms remain to be elucidated. In the present study, the effects of alantolactone on cell viability and apoptosis in RKO human colon cancer cells were investigated. Alantolactone treatment of RKO cells was found to result in dose-dependent inhibition of cell viability and induction of apoptosis, accompanied with the accumulation of reactive oxygen species (ROS) and the disruption of mitochondrial membrane potential. In addition, these effects were blocked with N-acetylcysteine, a specific ROS inhibitor. Western blotting indicated that exposure of RKO cells to alantolactone is associated with the downregulation of Bcl-2, induction of Bax and activation of caspase-3 and -9. These results indicated that a ROS-mediated mitochondria-dependent pathway is involved in alantolactone-induced apoptosis. From these observations, it was hypothesized that alantolactone may be used for the treatment of human colon cancer.

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Section: Introductionmentioning

confidence: 99%

Alantolactone induces apoptosis in RKO cells through the generation of reactive oxygen species and the mitochondrial pathway

Zhang

Bao

Wu³

et al. 2013

Molecular Medicine Reports

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“…Furthermore, mitochondria participate in the process of hypoxic response in different ways and the mitochondrial membrane potential (MMP) can reflect the function of mitochondria (Seol et al 2009;Wang et al 2009a, b;Snyder and Chandel 2009). It is reported that recovery of MMP can protect against ROS-mediated injuries such as hypoxia and ischemia (Iijima 2006;Zhou et al 2009). Therefore, the maintenance of MMP is very important to prevent from hypoxic injury.…”

Section: Introductionmentioning

confidence: 99%

The protective role of 5-HMF against hypoxic injury

Wu²,

Zhao³

et al. 2011

Cell Stress and Chaperones

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In an attempt to find new types of anti-hypoxic agents from herbs, we identified 5-hydroxymethyl-2-furfural (5-HMF) as a natural agent that fulfills the criterion. 5-HMF, the final product of carbohydrate metabolism, has favorable biological effects such as anti-oxidant activity and inhibiting sickling of red blood cells. The role of 5-HMF in hypoxia, however, is not yet. Our pilot results showed that pretreatment with 5-HMF markedly increased both the survival time and the survival rate of mice under hypoxic stress. The present study was aimed to further investigate the protective role of 5-HMF and the underlying mechanisms in hypoxic injury using ECV304 cells as an in vitro model. ECV304 cells pretreated with or without 5-HMF for 1 h were exposed to hypoxic condition (0.3% O 2 ) for 24 h and then cell apoptosis, necrosis, the changes of mitochondrial membrane potential (MMP) and the expressions of phosphorylation-extracellular signal-regulated kinase (p-ERK) were investigated. Pretreatment with 5-HMF markedly attenuated hypoxia-induced cell necrosis and apoptosis at late stage (p<0.01). Furthermore, pretreatment with 5-HMF rescued both the decline of the MMP and the increase of p-ERK protein under hypoxia. In a word, these results indicated that 5-HMF had protective effects against hypoxic injury in ECV304 cells, and its effects on MMP and p-ERK may be involved in the mechanism.

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“…Both organic and inorganic selenium was proved to be effective in restoring a GPx1 decrease, caused by exposure to phthalates used as additives in plastics, in LNCaP human prostatic cancer cell line [1]. According to Zhou et al, cadmium-induced increase in ROS production in LLC-PK 1 cells (20 mM Cd for 12 h) was fully reversed by pretreatment with selenium (5, 10, or 20 mM for 0.5 h as sodium selenite) [4]. The similar protective effect of pretreatment with sodium selenite was observed by Selvaraj et al in fish hepatoma cell line PLHC-1 exposed to arsenic trioxide.…”

Section: Discussionmentioning

confidence: 99%

“…Its presence in molecules of antioxidant enzymes: glutathione peroxidase and thioredoxin makes it one of the most important antioxidants [2][3][4]. The outcomes of research on its possible protective properties against prooxidative processes have seemed to be promising and encouraging.…”

Section: Introductionmentioning

confidence: 99%

Lithium as a prooxidant? A possible protective role of selenium – in vitro study

Musik

Kiełczykowska

Rajtar

et al. 2017

Ann Agric Environ Med.

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The protection of selenium on ROS mediated-apoptosis by mitochondria dysfunction in cadmium-induced LLC-PK1 cells

Cited by 171 publications

References 31 publications

Alantolactone induces apoptosis in RKO cells through the generation of reactive oxygen species and the mitochondrial pathway

Alantolactone induces apoptosis in RKO cells through the generation of reactive oxygen species and the mitochondrial pathway

The protective role of 5-HMF against hypoxic injury

Lithium as a prooxidant? A possible protective role of selenium – in vitro study

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