The protective effect of regucalcin against radiation-induced damage in testicular cells

Silva, Ana M.S.; Correia, Sara; Casalta-Lopes, João; Mamede, A.C.; Cavaco, José E.; Botelho, Maria Filomena; Socorro, Sílvia; Maia, Cláudio J.

doi:10.1016/j.lfs.2016.09.003

Cited by 22 publications

(17 citation statements)

References 68 publications

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“…3e ). These findings are further supported by a previous study conducted in the whole testis showing that Tg-RGN rats present lower apoptotic rates in consequence of the diminished activity of the apoptosis-effector caspase-3 34 . Also, it has been shown that RGN suppresses thapsigargin- and actinomycin D-induced apoptosis in SeT by modulating the expression and activity of key apoptotic and antiapoptotic factors 25 .…”

Section: Discussionsupporting

confidence: 86%

“…Previous findings from our research group confirmed that RGN is expressed both in SCs and germ cells and suggested that it may play a relevant role in spermatogenesis 32 , 33 . More recently, it was shown that RGN overexpression suppresses chemical- 25 or radiation-induced 34 apoptosis, and oxidative stress 35 in the testis, which suggests a protective role of this protein for germ cells. Although several approaches also have demonstrated that RGN modulates glucose handling by regulating the expression of several transporters and glycolytic enzymes 26 , 36 , 37 , RGN’s influence in the glycolytic metabolism, and glutaminolysis, of testicular cells is totally unknown.…”

Section: Introductionmentioning

confidence: 99%

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Glucose and glutamine handling in the Sertoli cells of transgenic rats overexpressing regucalcin: plasticity towards lactate production

Mateus

Feijó

Espínola

et al. 2018

Sci Rep

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Sertoli cells (SCs) possess the unparalleled ability to provide the germ line with growth factors and nutrients. Although SCs can oxidize amino acids, e.g., glutamine, they mostly metabolize glucose, producing high amounts of lactate, the germ cells preferential substrate. Regucalcin (RGN) is a calcium-binding protein that has been indicated as a regulator of cell metabolism. In this study, we investigated glucose and glutamine handling in the SCs of transgenic rats overexpressing RGN (Tg-RGN) comparatively with wild-type (Wt) littermates. Primary SCs isolated from adult Tg-RGN animals and maintained in culture for 24 hours, produced and exported more lactate, despite consuming less glucose. These observations were underpinned by increased expression of alanine transaminase, and augmented glutamine consumption, suggesting that alternative routes are contributing to the enhanced lactate production in the SCs of Tg-RGN rats. Moreover, lactate seems to be used by germ cells, with diminished apoptosis being detected in the seminiferous tubules of Tg-RGN animals cultured ex vivo. The obtained results showed a distinct metabolism in the SCs of Wt and Tg-RGN rats widening the roles assigned to RGN in spermatogenesis. These findings also highlighted the plasticity of SCs metabolism, a feature that would be exploited in the context of male infertility.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Glucose and glutamine handling in the Sertoli cells of transgenic rats overexpressing regucalcin: plasticity towards lactate production

Mateus

Feijó

Espínola

et al. 2018

Sci Rep

Self Cite

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“…Furthermore, apoptosis of germ cells has been reported as a mechanism responsible for infertility in irradiated testes [67,68]. Some studies have indicated that irradiation-mediated oxidative stress induces apoptosis primarily in adult mouse and rat germ cells [69,70,71,72]. Additionally, irradiation-induced germ cell apoptosis depends on activation of caspase-3 in three-month-old rats [72], concomitant with increased expression of caspase-8 and decreased expression of caspase-9 in adult rats and prepubertal mice, respectively [72,73] (Table 1, Figure 1).…”

Section: Radiotherapymentioning

confidence: 99%

Effects of Chemotherapy and Radiotherapy on Spermatogenesis: The Role of Testicular Immunology

Itoh

Sakabe

2019

IJMS

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Substantial improvements in cancer treatment have resulted in longer survival and increased quality of life in cancer survivors with minimized long-term toxicity. However, infertility and gonadal dysfunction continue to be recognized as adverse effects of anticancer therapy. In particular, alkylating agents and irradiation induce testicular damage that results in prolonged azoospermia. Although damage to and recovery of spermatogenesis after cancer treatment have been extensively studied, there is little information regarding the role of differences in testicular immunology in cancer treatment-induced male infertility. In this review, we briefly summarize available rodent and human data on immunological differences in chemotherapy or radiotherapy.

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“…Coглacнo peзультaтaм иccлeдoвaния учeных, дecтpук-ция cпepмaтoгoний и cпepмaтoцитoв пpи вoздeйcтвии иoнизиpующeгo излучeния oбуcлoвлeнo aктивaциeй aпoптoзa [33]. Тaкжe нeмaлoвaжным являeтcя тoт фaкт, чтo в cпepмaтoгeнных клeткaх измeняeтcя coдepжaниe ДНК, пpoиcхoдит cнижeниe чиcлa зpeлых пoлoвых клeтoк, a тaкжe диcкoopдинaция биoэнepгeтичecкoгo мeтa-бo лизмa в ткaнях ceмeнникoв [42]. Былo уcтaнoвлeнo, чтo измeнeния, пpoиcхoдящиe в ceмeнoнocнoм эпитeлии зpeлых живoтных в oтвeт нa вoздeйcтвиe низкoдoзoвoй paдиaции являютcя oбpaтимыми, пpичём cтeпeнь пoвpeждeния эпитeлиaльных клeтoк зaвиcит кaк oт дoзы излучeния, тaк и oт вoзpacтa живoтных [13].…”

Section: Peзультaты исследования и их Oбcуждeниeunclassified

Histomorphological Processes in the Testicular Tissue at Ionizing Radiation Exposure (Literature Review)

Uzbekov¹,

Hoshi²,

Chaizhunusova³

et al. 2017

IJAFR (МЖПФИ)

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Pacшиpяющиecя кoнтaкты чeлoвeчecтвa c иoнизиpующими излучeниями cтaвят их в pяд нaибoлee aктивных экoлoгичecких фaктopoв, влияющих нa пoлoвую cиcтeму, a тaкжe нa пoтoмcтвo oблучeнных poдитeлeй, нe утpaтивших cпocoбнocть к peпpoдукции. Нecмoтpя нa мнoгoчиcлeнныe литepaтуpныe дaнныe cвидeтeльcтвующиe o нeблaгoпpиятнoм влиянии paдиaции нa мужcкую peпpoдуктивную cиcтeму, в нacтoящee вpeмя вoзникaeт нeoбхoдимocть пpoвeдeния cpaвнитeльнoй мopфoлoгичecкoй oцeнки пoлoвых жeлeз лиц, пoдвepгaвшихcя вoздeйcтвию гaммa- и нeйтpoннoгo излучeния. Изучeниe нapушeний, вoзникaющих в ceмeнникaх кpыc пoд дeйcтвиeм иoнизиpующих излучeний, зaнимaeт oднo из вaжнeйших мecт в paдиoбиoлoгии, пocкoльку cпepмaтoгeнный эпитeлий oблaдaeт cпocoбнocтью к нeпpepывнoму oбнoвлeнию клeтoк, oблaдaющих paзличнoй чувcтвитeльнocтью к paдиaции, и являeтcя удoбнoй мoдeлью для иccлeдoвaния paдиaциoнных эффeктoв и oцeнки их пocлeдcтвий, кoтopыe мoгут вызвaть бecплoдиe и пepeдaтьcя cлeдующeму пoкoлeнию. Иccлeдoвaниe xapaктepa cтpуктуpных paccтpoйcтв в ткaнях ceмeнникoв пpи paзных уpoвнях и типaх paдиaциoннoгo вoздeйcтвия пoзвoлит иcпoльзoвaть peзультaты иccлeдoвaния для paзpaбoтки диaгнocтичecких кpитepиeв oцeнки влияния paдиaциoннoгo фaктopa. The expanding contacts of humankind with ionizing radiation installs them abreast among the most active environmental factors affecting the sexual system, as well as the posterity of irradiated parents, have not lost the ability to reproduce. Despite numerous published data showing the adverse radiation effects on the male reproductive system, currently there is a need making comparative morphological assessment the gonads of persons exposed to gamma- and neutron radiation. The study disorders arising in rat testes under the influence of ionizing radiation takes one of the most important positions in radiobiology, since spermatogenic epithelium characterized by capable of continuous cellular renewal having different sensitivity to radiation, and it is a convenient model for examination of radiation effects and assessment the consequence of them which can cause infertility and transmitted to the next generation. The research of structural nature of disorders in the testicular tissue at different levels and types of radiation exposure allows to use findings for develop the diagnostic criteria assessing the effect of radiation factor.

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The protective effect of regucalcin against radiation-induced damage in testicular cells

Cited by 22 publications

References 68 publications

Glucose and glutamine handling in the Sertoli cells of transgenic rats overexpressing regucalcin: plasticity towards lactate production

Glucose and glutamine handling in the Sertoli cells of transgenic rats overexpressing regucalcin: plasticity towards lactate production

Effects of Chemotherapy and Radiotherapy on Spermatogenesis: The Role of Testicular Immunology

Histomorphological Processes in the Testicular Tissue at Ionizing Radiation Exposure (Literature Review)

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