The protective effect of vitamin E on rats’ ovarian follicles following an administration of diazinon: An experimental study

Abstract

follicles following an administration of diazinon.Materials and Methods: A total of 30 adult female Wistar rats were divided into fivegroups: a control group (without any intervention), sham group (received only pureolive oil, as solvent), experimental group I (DZN+olive oil, 60 mg/kg), experimentalgroup II (vitamin E, 200 mg/kg), and experimental group III (DZN: 60 mg/kg+vitaminE: 200 mg/kg). All drugs were injected intraperitoneally, except vitamin E which wasadministrated by gavage. The animals were scarified after two weeks and left ovary wasused to measure proliferation of ovarian follicles. Tissues were analyzed by the PCNAtechnique and viewed with an optical microscope for evaluating cells proliferation.Results: The result of the present study revealed that the number of proliferative cellsin the experimental group I decreased significantly in contrast to the control groupin secondary and Graffian follicles (p< 0.001). The administration of vitamin E plusDZN significantly increased proliferative cells compared to the DZN group (p< 0.001).Primordial follicles showed that all study groups were lacking PCNA positive cells,which means no expression of PCNA in these follicles. The results of this study showedthat primary follicles in all study groups had a few and scattered PCNA positive cellswith no significant difference between the groups (p> 0.05).Conclusion: Results showed that DZN reduced proliferation in secondary and Graffianfollicles and vitamin E increased it. The results of this study suggested that vitamin Eby its antioxidant activity was able to improve the DZN-induced ovarian toxicity.

Hepatoprotective Effects of Soy Flour (Glycine max (L.) Merr.) Supplementation in Diazinon-Treated Wistar Rats

“…This structural change is caused by its reactive metabolite, free radicals diazoxon, which causes structural damage and disruption of cell membrane function, even cell death. 1,3,19 The results of this study are in line with previous researches showing a change in liver histopathology, such as sinusoid dilatation, narrowed central veins, parenchymal degeneration, hydropic degeneration, fatty degeneration, and necrosis. 1,20 Sinusoid dilatation in this study is in line with studies conducted by Al-Attar (2014) and Marzano et al (2015), which occurs due to pressure on the cell walls due to containment of the veins by toxic substances.…”

The role of oxidative stress in ovarian aging: a review

“…A clinical study found that VE combined with selenium supplementation significantly improved AMH index, antral follicle count (AFC) and mean ovarian volume (MOV) in patients with occult POI [227]. Experimental studies have found that VE intervention can enhance the antioxidant capacity of ovarian tissue, regulate endocrine hormones, reverse follicular atresia, and restore the normal vascular distribution of ovarian tissue [228]. VE was also shown to be involved in the normal antioxidant function of GSH-Px1 as an essential cofactor [229].…”

Tissues toxicity attenuation by vitamin E on oxidative damage induced by diazinon

“…The most important action of DZN compound toxicity is the inhibition of ChE activity and the accumulation of ACh in the peripheral and central nervous system leading to increased activation of nicotinic and muscarinic receptors [28].…”