The puff inducible in region 93D is responsible for the synthesis of the major ?heat-shock? polypeptide in Drosophila melanogaster

Scalenghe, Franco; Ritossa, Ferruccio

doi:10.1007/bf00399494

Cited by 17 publications

(11 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1B). A large deficiency, Df(3R)e Dz, deletes the chromosomal region between 93C and 93F, which includes the region where both genes map (Scalenghe and Ritossa 1977;Mohler and Pardue 1984). Males carrying marked wild-type chromosomes were treated with ethylmethane sulfonate (EMS), and the mutagenized chromosomes were screened for mutations that were lethal in trans to Df(3R)e Dz.…”

Section: Tin and Bap Have Essential Functionsmentioning

confidence: 99%

See 1 more Smart Citation

tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.

Azpiazu

Frasch²

1993

Genes & Development

697

550

View full text Add to dashboard Cite

show abstract

Section: Tin and Bap Have Essential Functionsmentioning

confidence: 99%

“…Single F1 flies with the mutagenized chromosome rutipa/TM1 were crossed with flies of the genotype Df(3R)eDZ/ TMI (Scalenghe and Ritossa 1977). The F2 generation was screened for lethality of rutipa/Df(3R)e Dz (absence of ebony flies).…”

Section: Ems Mutagenesismentioning

confidence: 99%

tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.

Azpiazu

Frasch²

1993

Genes & Development

697

550

View full text Add to dashboard Cite

show abstract

“…Previously, we reported (20) that embryos lacking the region at 93D where a large puff is inducible by HS do not show induction of the major 70,000-Dal HS polypeptide. This conclusion was based on the analysis of HS proteins from randomly chosen embryos generated by crossing flies heterozygous for deletions in this region, without knowledge of their genotypes.…”

Section: Resultsmentioning

confidence: 81%

“…De-letions Df(3R)eD7, Df(3R)eFl and Df(3R)eFS were obtained after x-ray treatment. Details of their cytology are given elsewhere (19,20). The lethal mutation 1(3)R12 was found after ethyl methanesulfonate treatment and is not complemented by the karD2 deletion.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Mutation generating a fragment of the major heat shock-inducible polypeptide in Drosophila melanogaster

Caggese¹,

Caizzi²,

Morea³

et al. 1979

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Drosophila melanogaster tissues carrying a third chromosome with the deletion Df(3R)karD2 make a 40,000-dalton (Dal) heat shock protein not made by wild type. The unusual polypeptide was inducible in every tissue examined. Tryptic peptide fingerprints showed it to include part of the 70,000-Dal major heat shock protein. Mapping experiments placed the mutation responsible for the 40,000-Dal protein at or close to the karD2 deletion. One break point of the deletion is in subdivision 87A, close to or at a heat shock locus that codes for the 70,000-Dal protein. The results are consistent with the possibility that this break point is within a gene for the 70,000-Dal protein, leaving only the initial portion of its coding sequence. This would specify the direction of transcription of the mutant gene as proximal to distal on the normal chromosome. The 87A heat shock locus should contain at least two genes for the 70,000-Dal protein, because embryos homozygous for the karD2 deletion and lacking the heat shock locus at 87C, which also codes for the 70,000-Dal protein, nevertheless produced both the 40,000-Dal and the 70,000-Dal proteins upon temperature elevation. Using the presence of the 40,000-Dal protein to monitor chromosome segregation, we found that embryos homozygous for deletions of the heat shock puff site at 93D exhibited a normal electrophoretic pattern of heat shock proteins.Heat shock (HS) and certain other treatments induce puffs at specific sites on the polytene chromosomes of Drosophila (1-3) and induce the synthesis of specific polypeptides (4-6). The response is not tissue specific (7) and occurs also in cultured cells (6). From induced cultured cells, messenger RNAs have been purified that hybridize in situ at the heat shock puff sites (6,(8)(9)(10). The presence of DNA sequences coding for the major heat shock polypeptide [70,000 daltons (Dal)] at both regions 87A and 87C is indicated by the finding that messenger RNA that codes for the 70,000-Dal polypeptide in a cell-free system (11, 12) cosediments with RNA that hybridizes to both regions (11). This conclusion has been verified by showing that cloned DNA fragments that hybridize at 87A and 87C also hybridize to messenger RNA for the 70,000-Dal polypeptide (13,14). Such localization is also supported by the observation that embryos homozygous for deletions of both 87A and 87C fail to produce the 70,000-Dal polypeptide (15). Both 87A and 87C appear to contain multiple copies for the genes for the 70,000-Dal HS polypeptide (16).We report here a mutation that leads to the induction after HS of an unusual polypeptide whose sequence is part of the major HS polypeptide. The mutation maps at region 87A and appears to result from a deletion that removes part of a gene for the 70,000-Dal HS protein. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. MATERIALS AND METHODS

show abstract

Rapid sequence divergence in a heat shock locus of Drosophila

1980

View full text Add to dashboard Cite

In situ hybridization of cRNA transcribed from cloned D. melanogaster heat shock sequences to D. hydei chromosomes has shown that the D. hydei locus 2--32 A corresponds to the D. melanogaster locus 87 A/C and the D. hydei locus 2--36 A to the D. melanogaster locus 95 D, while the D. hydei locus 4--81 B corresponds to the D. melanogaster locus 63 BC. No hybridization to D. hydei chromosomes was found with cRNA transcribed from a clone containing the alpha beta sequences encoded by the D. melanogaster locus 87 C. Neither D. melanogaster heat shock RNA nor D virilis heat shock RNA hybridized significantly to the D. hydei heat shock locus 2--48 B. Furthermore, D. hydei heat shock RNA did not hybridize to the cytological homologs of locus 2--48 B found in D. repleta or in D. virilis. D. hydei heat shock. RNA did hybridize to the cytological homologs of locus 2--48 B in D. neohydei and D. eohydei, both of which belong to the hydei subgroup.

show abstract

The puff inducible in region 93D is responsible for the synthesis of the major ?heat-shock? polypeptide in Drosophila melanogaster

Cited by 17 publications

References 20 publications

tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.

tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.

Mutation generating a fragment of the major heat shock-inducible polypeptide in Drosophila melanogaster

Rapid sequence divergence in a heat shock locus of Drosophila

Contact Info

Product

Resources

About