The purB gene of Escherichia coli K-12 is located in an operon

Green, Stephen M.; Malik, Tahir; Giles, Ian G.; Drabble, W. T.

doi:10.1099/13500872-142-11-3219

Cited by 23 publications

(15 citation statements)

References 46 publications

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“…3B, lane 3), which is consistent with a previous report (35). It was extractable with 0.1 M NaOH (Fig.…”

Section: Identification Of the Hfld Protein And Its Peripheral Associsupporting

confidence: 81%

“…Its homologs exist in several bacterial species including Hemophilus influenzae, Vivrio cholerae, and Pseudomonas aeruginosa (GenBank TM accession numbers, respectively, I64155, B82237, and D83317). No physiological role has been assigned for this dispensable gene (35). We also constructed a strain in which chromosomal hflD was disrupted by tet.…”

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

“…In contrast, pKH422 and pKH428 complemented hflD33 IPTG independently. It was suggested then that trmU, hflD, and purB comprise a single transcription unit (35).…”

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

“…We thus identified hflD as a new gene affecting the CII function of bacteriophage . (35). Its homologs exist in several bacterial species including Hemophilus influenzae, Vivrio cholerae, and Pseudomonas aeruginosa (GenBank TM accession numbers, respectively, I64155, B82237, and D83317).…”

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

“…pKH429 carried ycfC/orf-23 (GenBank TM accession number X59307; Ref. 35) as the sole intact chromosomal gene, which we designated hflD hereafter (Fig. 2).…”

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

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Revisiting the Lysogenization Control of Bacteriophage λ

Kihara

Akiyama

Ito

2001

Journal of Biological Chemistry

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Upon infection to the Escherichia coli cell, the genome of bacteriophage either replicates to form new progenies (lytic growth) or integrates into the host chromosome (lysogenization). The CII protein is a key determinant in the lysis-lysogeny decision. It is a short-lived transcription activator for the genes essential for lysogeny establishment. In this study, we isolated a new class of hfl (high frequency lysogenization) mutants of E. coli, using a new selection for enhancement of CIIstimulated transcription. The gene affected was termed hflD, which encodes a protein of 213 amino acids. An hflD-disrupted mutant indeed showed an Hfl phenotype, indicating that HflD acts to down-regulate lysogenization. HflD is associated peripherally with the cytoplasmic membrane. Its interaction with CII was demonstrated in vitro using purified proteins as well as in vivo using the bacterial two-hybrid system. Pulsechase examinations demonstrated that the HflD function is required for the rapid in vivo degradation of CII, although it interfered with FtsH-mediated CII proteolysis in an in vitro reaction system using detergent-solubilized components. We suggest that HflD is a factor that sequesters CII from the target promoters and recruits it to the membrane where the FtsH protease is localized.

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“…3B, lane 3), which is consistent with a previous report (35). It was extractable with 0.1 M NaOH (Fig.…”

Section: Identification Of the Hfld Protein And Its Peripheral Associsupporting

confidence: 81%

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

“…In contrast, pKH422 and pKH428 complemented hflD33 IPTG independently. It was suggested then that trmU, hflD, and purB comprise a single transcription unit (35).…”

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

“…pKH429 carried ycfC/orf-23 (GenBank TM accession number X59307; Ref. 35) as the sole intact chromosomal gene, which we designated hflD hereafter (Fig. 2).…”

Section: Isolation Of a New Class Of Mutations That Elevates Cii-depementioning

confidence: 99%

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