The reaction of phloroglucinol in the presence of acid with lignin-containing materials

Morphological and histological differences among three types of component roots and their differential contribution to water uptake in the rice root system

“…The digitized images were taken at the same time by using CCD camera (DP21, Olympus, Tokyo, Japan) together with non-stained samples. To observe the deposition of lignin that is a component of tertiary wall, cross sections were stained for 10 min with phloroglucinol (final concentration; 1% (w/v)) which was mixed with 75% ethanol at room temperature and then 6 N HCl was dropped on the samples (Wiesner reaction, Davidson et al, 1995). Sections were viewed under a light microscope (IX70, inverted fluorescence microscope, Olympus, Tokyo, Japan) and the digitized images were taken at the same time by using a CCD camera (DP21, Olympus, Tokyo, Japan) together with non-stained samples.…”

Diverse forms of xylem-Like cells and strand formation in Xylogenic Eucalyptus bosistoana callus culture

“…After incubation, the cells were washed three times with deionised water and then stored in 70% (v/v) ethanol at 4-6 °C until further analysis. Before acid maceration, lignified cells in the xylogenic callus were stained using phloroglucinol-HCL (Davidson et al 1995), and after maceration, the cells were stained with toluidine blue-O or TBO (O'Brien et al 1964).…”

“…XLCs were categorised according to their secondary cell wall thickening patterns and colour reaction with different stains. The staining of cells red in the presence of phloroglucinol HCL would suggest the presence of cinnamyl aldehyde residues in lignin polymers (Davidson et al 1995). After acid maceration, the cells were then stained with TBO (O'Brien et al 1964), the lignified cells would exhibit blue to greenish blue colour in the secondary cell wall while the staining of the cells purple would suggest the presence of pectin in the primary cell wall.…”

“…Xylem elements or vessel members are mainly found in the vascular tissues of angiosperms such as eucalypts and serve as a pipeline or conducting tube through which water and mineral nutrients are transported throughout the plant body (Déjardin et al 2010). During primary growth of the plant body, vascular tissues including vessel members are differentiated from the procambium, while during secondary growth they arise from the vascular cambium (Watson 1976).…”

The Employment of a Removable Chitosan‐Derivatized Polymeric Sensitizer in the Photooxidation of Polyhydroxylated Water‐Pollutants

“…The oxidizable target molecules we utilized in this work for modeling the water contaminants were three trihydroxybenzene derivatives (THBs). Due to their profuse use with industrial purposes THBs have been frequently found as contaminants in waste‐waters .…”