The recruitment of the U5 snRNP to nascent transcripts requires internal loop 1 of U5 snRNA

Kim, Rebecca; Paschedag, Joshua; Novikova, Natalya; Bellini, Michel

doi:10.1007/s10577-012-9326-8

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…[26,27] Crucially, targeting of fluorescent protein fusions and tagged small RNAs to the lateral loops allow in vivo imaging of nascent RNP formation and spliceosomal RNP recruitment. [27][28][29] Long-term imaging of the individual transcription loops in living chromosomes under the conditions in which nascent RNA synthesis is ongoing enabled dynamic studies of the elongation of nascent transcript. [28] These studies suggested that the extension of transcription loops requires continued coverage with nascent RNA.…”

Section: Lampbrush Chromosomes As a Unique Model For Visualization Of...mentioning

confidence: 99%

Lampbrush chromosome studies in the post‐genomic era

2023

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Extraordinary extended lampbrush chromosomes with thousands of transcription loops are favorable objects in chromosome biology. Chromosomes become lampbrushy due to unusually high rate of transcription during oogenesis. However, until recently, the information on the spectrum of transcribed sequences as well as genomic context of individual chromomeres was mainly limited to tandemly repetitive elements.Here we briefly outline novel findings and future directions in lampbrush chromosome studies in the post-genomic era. We emphasize the fruitfulness of combining genomewide approaches with microscopy imaging techniques using lampbrush chromosomes as a remarkable model object. We believe that new data on the spectrum of sequences transcribed on the lateral loops of lampbrush chromosomes and their structural organization push the boundaries in the discussion of their biological role. Also see the video

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Section: Lampbrush Chromosomes As a Unique Model For Visualization Of...mentioning

confidence: 99%

Lampbrush chromosome studies in the post‐genomic era

2023

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A remarkable career in science—Joseph G. Gall

2013

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A festive group of ∼150 current and former students, postdoctoral and other associates, and colleagues gathered during the weekend of April 12-14, 2013 to celebrate Joe Gall's 85th birthday. The gathering, hosted by the Carnegie Institution for Science, Department of Embryology (Allan Spradling, Director) and organized by a group of Joe's current and former students (Zehra Nizami, Alison Singer, Ji-Long Liu, Virginia Zakian, Susan Gerbi), was held in Baltimore, MD. Dinners and symposia extending over 3 days celebrated Joe's scientific findings over the years, together with those of his former students, postdoctoral fellows, and other associates (see program at https://sites.google.com/site/gallsymposium2013/ ).

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Identification of critical residues in spliceosomal protein Dib1 (939.6)

2014

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Pre‐messenger RNA in eukaryotic cells must have its introns removed in order to be able toproduce functional proteins. This removal of introns is facilitated by a macromolecule known as the spliceosome. The spliceosome is a large complex of five snRNA and ~80 proteins that orients the pre‐mRNA such that two transesterification reactions are catalyzed resulting in the removal of introns from pre‐mRNA resulting in a mature mRNA. The mRNA can then be translated in to a functional protein. Mutations in the spliceosome and the proteins that regulate the spliceosome can have drastic effects and lead to diseases such as Retinitis Pigmentosa. Dib1 is an evolutionarily conserved protein in the spliceosome associated with the U5 snRNP in the spliceosome and is required for cell viability. Although the human homolog, Dim1, has been crystalized its role still remains unknown. In order to identify Dib1’s function, we have created point mutations in the protein and analyzed their effects on cell viability. We have identified a variety of temperature sensitive and lethal dib1 mutants in Saccharomyces cerevisiae. We are characterizing these mutants to determine the effect these mutations have on Dib1 protein structure and their overall impact on splicing activity. This study will identify the role of critical residues in Dib1 in pre‐mRNA splicing.

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The recruitment of the U5 snRNP to nascent transcripts requires internal loop 1 of U5 snRNA

Cited by 3 publications

References 30 publications

Lampbrush chromosome studies in the post‐genomic era

Lampbrush chromosome studies in the post‐genomic era

A remarkable career in science—Joseph G. Gall

Identification of critical residues in spliceosomal protein Dib1 (939.6)

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