The Regulation of Nitrate Assimilation in Yeast

Hipkin, C. R.

doi:10.1007/978-3-642-75812-6_28

Cited by 7 publications

(11 citation statements)

References 18 publications

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“…The chemical characteristics of nitrate and nitrite make necessary the existence of transport systems to carry these molecules to the interior of the cells in which they are utilised. Hipkin [7] provided the ¢rst evidence on the connection between nitrate induced protein synthesis and nitrate uptake in yeast. Shortly before, Eddy and Hopkins [8] showed that in Candida utilis nitrate uptake is concomitant with the entrance of two equivalents of protons and the exit of one equivalent of K þ .…”

Section: Nitrate and Nitrite Transportmentioning

confidence: 99%

Assimilation of nitrate by yeasts

Siverio¹

2002

FEMS Microbiology Reviews

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Nitrate assimilation has received much attention in filamentous fungi and plants but not so much in yeasts. Recently the availability of classical genetic and molecular biology tools for the yeast Hansenula polymorpha has allowed the advance of the study of this metabolic pathway in yeasts. The genes YNT1, YNR1 and YNI1, encoding respectively nitrate transport, nitrate reductase and nitrite reductase, have been cloned, as well as two other genes encoding transcriptional regulatory factors. All these genes lie closely together in a cluster. Transcriptional regulation is the main regulatory mechanism that controls the levels of the enzymes involved in nitrate metabolism although other mechanisms may also be operative. The process involved in the sensing and signalling of the presence of nitrate in the medium is not well understood. In this article the current state of the studies of nitrate assimilation in yeasts as well as possible venues for future research are reviewed.

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Section: Nitrate and Nitrite Transportmentioning

confidence: 99%

Assimilation of nitrate by yeasts

Siverio¹

2002

FEMS Microbiology Reviews

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“…In general, nitrate is the inducer of NR synthesis while reduced nitrogen sources are repressors. For reviews on NR regulation in yeast, plants and filamentous fungi see Hipkin (1989), Solomonson & Barber (1990) and Marzluf (1993).…”

Section: Introductionmentioning

confidence: 99%

Nitrite causes reversible inactivation of nitrate reductase in the yeast Hansenula anomala

González

Ávila

et al. 1994

Microbiology

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“…I N T R O D I" <' TIO N Se\'eral yeasts are able to assimilate tiitrate as a nitrogen source, and the physiology and enzytnology of nitrate assimilation of some species, in genera such as Candida, Hansenula and Rhodotorula, is well understood (see reviews by Hipkin, 1989Hipkin, , 1990. Nitrite, an intermediate in the reductive assimilation of nitratc-nitrogen into ammonium-nitrogen, is also a potential source of nitrogen for these organisms and appears to play an important role in the regulation of nitrate assimilation (Gonzalez et al, 1994).…”

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confidence: 99%

Nitrite assimilation by the yeast Candida nitratophila

1996

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S L: M M A R yNitrite uptake in the nitrate-assimilating yeast, Candida nitratophila, was inhibited completely by addition of nitrate or ammonium to cultures at pH SO. Nitrite assimilation m these cultures was also completely dependent on the availability of a suitable carbon source (glucose). In cultures at pH 5'0, neither nitrate nor ammonium inhibited nitrite uptake completely. In these cultures, some nitrite was assimilated in the absence of a carbon source. Carbon-deficient cultures at pH 5'0 were able to reduce nitrite to ammonium but excreted this ammonium without further assimilation. E\idence for the uptake of nitrite by passive diffusion and by active transport via the nitrate uptake system is discussed.

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The Regulation of Nitrate Assimilation in Yeast

Cited by 7 publications

References 18 publications

Assimilation of nitrate by yeasts

Assimilation of nitrate by yeasts

Nitrite causes reversible inactivation of nitrate reductase in the yeast Hansenula anomala

Nitrite assimilation by the yeast Candida nitratophila

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