The Relative Rate of Immunoglobulin Gamma 1 Fragmentation

Biobetters

“…However, hinge region hydrolysis rates for intact antibody and F(ab)2 fragments were similar, indicating that the Fc region does not protect the hinge region against direct hydrolysis. Similar observations were made by Kamerzell et al ( 2010 ). pH also affects the site of fragmentation, possibly by affecting antibody conformation.…”

Effects of Temperature and Osmolytes on Competing Degradation Routes for an IgG1 Antibody

“…Because fragmentation is a chemical degradation route, one would expect it to follow Arrhenius or nearly Arrhenius behavior for a given choice of pH and buffer/excipient conditionshence the straight line for the schematic temperature dependence of fragmentation in Figure 5a. 29,34 In the present case, fragmentation rates appear to be independent of excipient conditions. Although the precise mechanism of fragmentation is not known for this IgG1, comparison with other IgG1 antibodies 29,34 suggests that under these mildly acidic conditions the activation energy for fragmentation is expected to be roughly 20 kcal/mol.…”

“…Because fragmentation is a chemical degradation route, one would expect it to follow Arrhenius or nearly Arrhenius behavior for a given choice of pH and buffer/excipient conditions—hence the straight line for the schematic temperature dependence of fragmentation in Figure a . In the present case, fragmentation rates appear to be independent of excipient conditions.…”

“…In the present case, fragmentation rates appear to be independent of excipient conditions. Although the precise mechanism of fragmentation is not known for this IgG1, comparison with other IgG1 antibodies suggests that under these mildly acidic conditions the activation energy for fragmentation is expected to be roughly 20 kcal/mol. The slope for the fragmentation line in Figure (both panels) is drawn to roughly capture this approximate activation energy, and this magnitude for the fragmentation activation energy is consistent with the observations above regarding fragmentation at 40°C versus higher and lower temperature conditions.…”

Metal ion interactions with mAbs: Part 1

“…The identity of these SEC peaks for mAb 1 were previously determined using mass spectrometry. 33 Addition of Cu 2C increased the rate of mAb 1 fragmentation as shown in Figure 3A, resulting in generation of the Fab arm and the single-arm antibody (Fab C Fc) from the parent mAb 1. Increased fragmentation with the addition of Cu 2C to mAb 1 in sodium acetate buffer was clearly observed and was not detected when formulated with histidine acetate buffer (Fig.…”