The reliability of endothelial progenitor cells (CD34+, KDR+, and CD34+/KDR+) in the diagnosis of coronary artery disease and its severity

Sewify, Eman M.; Afifi, Ola; Soliman, Mona; Ziada, Ahmad I.

doi:10.7123/01.ejh.0000419284.25275.82

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“…Endothelial progenitor cells (EPC) are of immense interest in vascular diseases and regenerative medicine for their ability to promote postnatal neovasculogenesis. Circulating EPCs is reported to be lower in individuals with vascular diseases like coronary artery disease (CAD) and peripheral arterial disease (PAD) compared to healthy individuals (Vasa et al, 2001) (Sewify, 2020) (Vemparala et al, 2013) (Bitterli et al, 2015). In vitro expansion of EPCs from peripheral blood has shown two types of EPCs -early EPCs, also known as circulating angiogenic cells (CAC) and late EPCs, also known as outgrowth endothelial cells (OECs) or endothelial colony forming cells (ECFCs) based on their appearance in culture, cell morphology, expression of cell surface markers and ability to promote in vitro angiogenesis (Medina et al, 2017) (Cheng et al, 2013).…”

Section: Endothelial Colony Forming Cells (Ecfcs) Tetrahydrobiopterin...mentioning

confidence: 99%

Endothelial colony forming cells’ tetrahydrobiopterin level in coronary artery disease patients and its association with circulating endothelial progenitor cells

Sen

Thakkar

Vincent

et al. 2022

Can. J. Physiol. Pharmacol.

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Endothelial colony forming cells (ECFCs) participates in neovascularization. Endothelial nitric oxide synthase (eNOS) derived NO. helps in homing of Endothelial progenitor cells (EPC) at the site of vascular injury. The enzyme cofactor tetrahydrobiopterin (BH4) stabilizes the catalytic active state of eNOS. Association of intracellular ECFCs biopterins and ratio of reduced to oxidized biopterin (BH4:BH2), with circulatory EPCs and ECFCs functionality has not been studied. We investigated ECFCs biopterin levels and its association with circulatory EPCs as well as ECFCs proliferative potential in terms of day of appearance in culture. Circulatory EPCs were enumerated by flowcytometry in 53 Coronary Artery Disease (CAD) patients and 42 controls. ECFCs were cultured, characterized and biopterin levels assessed by HPLC. Appearance of ECFCs colony and their number were recorded. Circulatory EPCs were significantly lower in CAD and ECFCs appeared in 56% and 33% of CAD and control subjects respectively. Intracellular BH4 and BH4:BH2 were significantly reduced in CAD. BH4:BH2 was positively correlated with circulatory EPCs (p = 0.01), and negatively with day of appearance of ECFCs (p = 0.04). Circulatory EPCs negatively correlated with ECFCs appearance (p = 0.02). These findings suggest the role of biopterins in maintaining circulatory EPCs and functional integrity of ECFCs.

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Section: Endothelial Colony Forming Cells (Ecfcs) Tetrahydrobiopterin...mentioning

confidence: 99%

Endothelial colony forming cells’ tetrahydrobiopterin level in coronary artery disease patients and its association with circulating endothelial progenitor cells

Sen

Thakkar

Vincent

et al. 2022

Can. J. Physiol. Pharmacol.

View full text Add to dashboard Cite

show abstract

The reliability of endothelial progenitor cells (CD34+, KDR+, and CD34+/KDR+) in the diagnosis of coronary artery disease and its severity

Cited by 1 publication

References 0 publications

Endothelial colony forming cells’ tetrahydrobiopterin level in coronary artery disease patients and its association with circulating endothelial progenitor cells

Endothelial colony forming cells’ tetrahydrobiopterin level in coronary artery disease patients and its association with circulating endothelial progenitor cells

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