The respiratory burst response of surface-adhering leukocytes. A key to tissue engineering

Nygren, Håkan; Broberg, Marita; Eriksson, Cecilia; Sahlin, Herman; Yahyapour, Noushin

doi:10.1016/s0927-7765(00)00216-2

Cited by 13 publications

(10 citation statements)

References 85 publications

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“…Some results indicate the possible relation of oxidative stress with cell adhesion [30,31]. Recent studies have revealed that crucial cellular signalling pathways are regulated by the extra and intracellular red-ox status and that ROS function as intercellular signal molecules [32]. In order to evaluate the intracellular ROS production, cells cultured on either PCL films (untreated and treated with NaOH) or TCP were loaded with the probe DCFH at different culture times.…”

Section: Resultsmentioning

confidence: 99%

Transitory oxidative stress in L929 fibroblasts cultured on poly(ε-caprolactone) films

et al. 2005

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Section: Resultsmentioning

confidence: 99%

Transitory oxidative stress in L929 fibroblasts cultured on poly(ε-caprolactone) films

et al. 2005

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“…Improving biomaterial performance generally concerns the minimization of material-induced inflammatory responses (Anderson, 2004). One of the most important cellular mediators of acute inflammation is the polymorphonuclear leukocyte (PMNL or neutrophil), which within minutes reaches the site of implantation (Nygren et al, 2001). In a process referred to as frustrated phagocytosis neutrophils try to engulf and degrade the foreign material, all under the secretion of harmful substances such as different reactive oxygen species (ROS) (Nygren et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…One of the most important cellular mediators of acute inflammation is the polymorphonuclear leukocyte (PMNL or neutrophil), which within minutes reaches the site of implantation (Nygren et al, 2001). In a process referred to as frustrated phagocytosis neutrophils try to engulf and degrade the foreign material, all under the secretion of harmful substances such as different reactive oxygen species (ROS) (Nygren et al, 2001). The acute inflammatory response may progress into a state of chronic inflammation, ultimately leading to implant failure.…”

Section: Introductionmentioning

confidence: 99%

Mucin Coatings Suppress Neutrophil Adhesion to a Polymeric Model Biomaterial

Sandberg

Carlsson

Ott

2007

Microscopy Res & Technique

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Following our recent study on the fractionation, characterization, and model adsorption of mucins derived from bovine salivary glands (BSM), porcine stomach scrapings (PGM), and human whole saliva (MG1), we here present a microscopic evaluation of the interactions between mucin-coated substrates and human neutrophils. Our results show that surface-coating with BSM, PGM, and MG1 can be effectively used to suppress the adhesion of neutrophils to a polymeric model biomaterial (Thermanox). Neutrophil morphologies found on Thermanox substrates coated with mucins resemble those observed for nonactivated neutrophils found in circulation. Notably, low neutrophil adhesion can be obtained at a significantly lower coating concentration (0.125 mg/mL) for the compositionally complex MG1 mucin than for the relatively pure BSM and PGM mucins (1 mg/mL). Furthermore, since coating at a low BSM and PGM concentration (0.25 mg/mL) results in higher cell counts and more spread cells than in the high-concentration case, we suggest that dense mucin surface packing is critical for good coating performance. In conclusion, the present study demonstrates how mucins from three different sources, of different compositional and structural status, efficiently can be used to suppress neutrophil adhesion and activation. This finding makes them potent candidates for use as biomaterial coatings.

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“…Here, we focus on the neutrophils, which are cellular members of the innate immune system and important mediators of biomaterial-induced inflammation [11]. Earlier studies have pointed to important in vivo relationships between mucins and neutrophils; e.g.…”

Section: Introductionmentioning

confidence: 99%

Interactions between human neutrophils and mucin-coated surfaces

Sandberg

Carlsson

Ott

2008

J Mater Sci: Mater Med

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Recently, we showed microscopically that bovine (BSM), porcine (PGM) and human (MG1) mucin coatings could suppress the adhesion of neutrophils to a polyethylene terephthalate-based model biomaterial (Thermanox). Here, using the release of reactive oxygen species (ROS) as a marker of material-induced neutrophil activation, the strong surface-passivating effects of these mucin coatings were corroborated. Under optimal adsorption conditions, all mucin species performed equally well, thus indicating a high degree of functional homology between the mucins. Cell adhesion and morphology correlated well with the release of ROS. Quartz crystal microbalance (QCM-D) analysis linked low neutrophil activation to efficient mucin surface-shielding. Interestingly, the shielding power appeared equal for thick expanded and thin compact mucin coatings. Combined mucin-serum coatings were found to be highly surface-passivating. Particularly, since our data suggested partly synergistic mucin-serum action, we highlight the possibility that pre-adsorbed mucins could provide favorable support for adsorbing host components.

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The respiratory burst response of surface-adhering leukocytes. A key to tissue engineering

Cited by 13 publications

References 85 publications

Transitory oxidative stress in L929 fibroblasts cultured on poly(ε-caprolactone) films

Transitory oxidative stress in L929 fibroblasts cultured on poly(ε-caprolactone) films

Mucin Coatings Suppress Neutrophil Adhesion to a Polymeric Model Biomaterial

Interactions between human neutrophils and mucin-coated surfaces

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