Transitory oxidative stress in L929 fibroblasts cultured on poly(ε-caprolactone) films

Serrano, María Concepción; Pagani, Raffaella; Peña, J. L.; Portolés, María Teresa

doi:10.1016/j.biomaterials.2005.02.039

Cited by 39 publications

(38 citation statements)

References 36 publications

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“…It has observed that ROS content of L929 fibroblasts cultured on PCL films has similar to control after 24 h. However, in cells cultured on PCL, this parameter has increased after 4 days in culture. After 7 days, ROS content of cells has decreased [31]. Our findings were in agreement with this study.…”

Section: Discussionsupporting

confidence: 95%

“…Some results indicate the possible relation of oxidative stress with cell adhesion [31,35,36]. It has observed that ROS content of L929 fibroblasts cultured on PCL films has similar to control after 24 h. However, in cells cultured on PCL, this parameter has increased after 4 days in culture.…”

Section: Discussionmentioning

confidence: 75%

“…For this reason, we measured the oxidative damage on lipid and protein due to detected MDA and AOPP levels, respectively, and SOD activity as a cell defense system. Cell oxidative stress is a quite new and interesting topic in tissue engineering research, and not many studies have been performed in order to evaluate oxidative stress parameters related to the effects of the scaffolds treatment [31]. Serrano et al showed that a stimulation of mitochondrial redox activity is produced by the contact with poly(ε-caprolactone) (PCL) film [25] which PCL is a biodegradable and biocompatible polymer.…”

Section: Discussionmentioning

confidence: 99%

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Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

Demirbilek

Karahaliloğlu

et al. 2011

Appl Biochem Biotechnol

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Oxidative stress may produce high level of reactive oxygen species (ROS) following cell exposure to endogenous and exogenous factors. Recent experiments implicate oxidative stress as playing an essential role in cytotoxicity of many materials. The aim of this study was to measure intracellular malondialdehyde (MDA), advanced oxidation protein product (AOPP) levels, and superoxide dismutase (SOD) activities of L929 fibroblasts cultured on PDLLA, polyethylene glycol (PEG), or ethylenediamine (EDA) grafted PDLLA by plasma polymerization method. Cell proliferation on these scaffolds was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The study showed that MDA, AOPP levels, and SOD activities in L929 fibroblast cells cultured on all scaffolds were significantly different compared to the control group and each other. The highest MDA (0.42 ± 0.76 nmol/mg protein), AOPP (14.99 ± 4.67 nmol/mg protein) levels, and SOD activities (7.49 ± 3.74 U/mg protein) were observed in cells cultured on non-modified scaffolds; meanwhile, the most cell proliferation was obtained in EDA-modified scaffolds (MDA 0.15 ± 0.14 nmol/mg protein, AOPP 13.12 ± 3.86 nmol/mg protein, SOD 4.82 ± 2.64 U/mg protein). According to our finding, EDA- or PEG-modified scaffolds are potentially useful as suitable biomaterials in tissue engineering.

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Section: Discussionsupporting

confidence: 95%

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

Demirbilek

Karahaliloğlu

et al. 2011

Appl Biochem Biotechnol

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“…These facts can be explained by considering what is happening to the cells: a transitory, but significant, oxidation stress has been observed, during short culture times, in the same type of cells employed in this work. 28 The stimulation of mitochondrial activity can induce the synthesis of ROS, which are formed in mitochondria when energy metabolism is compromised. ROS include singlet oxygen and hydrogen peroxide, as well as free radicals such as superoxide anion and hydroxyl radical, being able to impair the function of several cellular components, including proteins, nucleic acids, and lipids.…”

Section: Degradation In the Presence Of Fibroblastmentioning

confidence: 99%

“…27 In addition, it was noticed that this alkaline treatment generated a transitory oxidative stress of less significance when compared with nontreated membranes. 28 This phenomenon, caused by a stimulation of mitochondrial activity that generates reactive oxygen species (ROS) (singlet oxygen, hydrogen peroxide, superoxide anion, hydroxyl radical. ), 29 has been related not only with apoptosis 30 but also with cells adhesion.…”

Section: Introductionmentioning

confidence: 99%

Alkaline‐treated poly(ϵ‐caprolactone) films: Degradation in the presence or absence of fibroblasts

Peña

Corrales

Izquierdo‐Barba

et al. 2005

J Biomedical Materials Res

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In the first stage, we observed the study of the degradation behavior of alkaline-treated poly(epsilon-caprolactone) (PCL) in two biologically-related media: phosphate buffered saline (PBS) and Dulbecco's modified Eagle's medium (DMEM) for 18 months, finding a much accelerated degradation in the last one. As expected, the degradation in the presence of cells is much pronounced even considering that the study is limited to 6 months. The characterization of the degraded substrates by chemiluminescence (CL) allows to explain the modifications of the substrate and their relations with transitory oxidative stress phenomena described in the fibroblasts seeded onto the PCL membranes.

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A new application of avocado oil to enrich the biological activities of polycaprolactone membranes for tissue engineering

Yurtsever,

Aydoğan,

İyigündoğdu

et al. 2024

Biopolymers

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The metabolites synthesized by plants to protect themselves serves as natural antimicrobial agents used in biomaterials. In this study, avocado oil (AO), was incorporated as a plant source and natural antimicrobial agent into polycaprolactone (PCL) membranes. The effects of varying AO ratios (25, 50, and 100 wt%.—PCL@25AO, PCL@50AO, PCL@100AO) on PCL membrane morphology, chemical structure, wettability, antimicrobial activity, and cell viabilities were investigated. It was demonstrated that the AO acts as a pore‐forming agent in solvent‐casted membranes. Young's modulus of the membranes varied between 602.68 and 31.92 MPa and more flexible membranes were obtained with increasing AO content. Inhibition zones of AO were recorded between 7.86 and 13.97 mm against clinically relevant microbial strains including bacteria, yeast, and fungi. Antimicrobial activity of AO was retained in PCL membranes at all ratios. Resazurin assay indicated that PCL@25AO membranes were cytocompatible with mouse fibroblast cells (L929 cell line) on day 6 showing 72.4% cell viability with respect to neat PCL membranes. Viability results were supported by scanning electron microscopy images and DAPI staining. The overall results of this study highlight the potential of PCL@25AO membranes as a biomaterial with antimicrobial properties, cytocompatibility, and mechanical strength suitable for various biomedical applications.

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Transitory oxidative stress in L929 fibroblasts cultured on poly(ε-caprolactone) films

Cited by 39 publications

References 36 publications

Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

Alkaline‐treated poly(ϵ‐caprolactone) films: Degradation in the presence or absence of fibroblasts

A new application of avocado oil to enrich the biological activities of polycaprolactone membranes for tissue engineering

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