The results of the comparative amino acid analysis of species of hedysarum growing in the North Caucasus

Imachueva, Djavgarat R.; Serebryanaya, Fatima Kazbekovna

doi:10.21626/vestnik/2020-1/10

Cited by 2 publications

(2 citation statements)

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“…Numerous studies report on the content of amino acids in representatives of such families as Nettle (Urticaceae) [12,13], Oleaster (Elaeagnaceae) [14], Leguminosae (Fabaceae) [15,16], and others [17,18]. It has been proven that amino acids are capable of accumulating both in aboveground plant organs and in underground ones.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Free Amino Acid Profiles in Underground Organs of Several Species of the Genus Rumex During Different Phases of the Vegetation Cycle

Poluyanov,

Matvienko,

Sokolova

et al. 2024

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Amino acids (AA), the primary metabolites in plants, play a crucial role in various physiological processes, including the synthesis of phenolic compounds. Drug products and dietary supplements made from medicinal plants can become a rich source of both nonessential and essential amino acids. High levels of free amino acids found in herbal raw material often indicate the presence of biotic and abiotic stress in the plants. Therefore, understanding the dynamics of bioactive compound accumulation in plants throughout their phenological phases of development is critical to optimizing their potential health benefits.Aim. To compare qualitative composition and dynamics of AA accumulation in the underground organs of four representatives of the Rumex genus: R. confertus, R. crispus L., R. obtusifolius L., R. aquaticus L. of three different vegetative phases.Materials and methods. Water extracts from underground organs of the studied plants were analyzed using two different methods for qualitative and quantitative analysis. Extracts were applied to the chromatographic plates TLS Silica gel 60 F254 (Merk, Germany) 20 × 20 cm with a micro-syringe (LLC "Tsvet", Russia). After elution, the plates were treated with a 2 % ninhydrin solution. Quantitative analysis was carried out using the SF-2000 spectrophotometer (LLC "OKB Spectr", Russia).Results and discussion. The amino acid profile of the underground organs of R. confertus, R. crispus L., R. obtusifolius L., R. aquaticus L. during three vegetative phases was determined using the TLC method; the quantitative analysis performed using spectrophotometry.Conclusion. The most diverse amino acid (AA) profiles were found in the withering phase in all Rumex species, with up to 9 amino adsorption zones. In contrast, the least diverse AA profiles were observed in the flowering phase, ranging from 2 to 4 adsorption zones. The quantitative content of AA was lowest in the flowering phase, increased during the regrowth phase, and peaked in the withering phase. However, R. crispus L. showed an unusual pattern, with the highest quantitative content of AA detected in the regrowth phase.

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Section: Introductionmentioning

confidence: 99%

Comparative Study of Free Amino Acid Profiles in Underground Organs of Several Species of the Genus Rumex During Different Phases of the Vegetation Cycle

Poluyanov,

Matvienko,

Sokolova

et al. 2024

Razrabotka i registraciâ lekarstvennyh sredstv

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“…According to many researchers, Hedysarum alpinum L. can be considered as a promising object for import substitution and production of xanthone glycoside mangiferin [16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Culturation of Hedysarum Alpinum L. Cell Culture Under in Vitro Conditions

2023

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The purpose of the research is to develop a process for the continuous cultivation of Alpine Hedysarum cells in larger vessels (fermenters). The studies were carried out in the laboratory of biotechnology of the Federal State Budgetary Scientific Institution VILAR in 2016–2022. As a result of the experiments, a cell line was obtained from the cotyledon of a seedling of the alpine hedysarum plant, labeled as the Hedysarum alpinum (C) 2016 strain. both physiologically and morphologically. The duration of growth of the suspension culture of Hedysarum alpinum (C) 2016 corresponded to 16-20 days of cultivation, the maximum growth of the culture was 3.4 g/l of the nutrient medium, which corresponds to 24% of the growth of the cell culture in flasks on a shaker. The cell culture grown in the fermenter does not develop uniformly, as evidenced by the two peaks of biomass growth on the graph of the absolute growth rate. The factor of physiological heterogeneity of the suspension culture of Hedysarum alpinum (C) 2016 was noted, which can affect the final results on the accumulation of biomass and its biosynthetic activity. The isoflavone glycoside ononin, 7-O-(β-glucopyranosyioloxy)-4 methoxyisoflavone, was found in the cell culture of Alpine Hedysarum alpinum.

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The results of the comparative amino acid analysis of species of hedysarum growing in the North Caucasus

Cited by 2 publications

References 4 publications

Comparative Study of Free Amino Acid Profiles in Underground Organs of Several Species of the Genus Rumex During Different Phases of the Vegetation Cycle

Comparative Study of Free Amino Acid Profiles in Underground Organs of Several Species of the Genus Rumex During Different Phases of the Vegetation Cycle

Culturation of Hedysarum Alpinum L. Cell Culture Under in Vitro Conditions

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