The rodent granular convoluted tubule cell ? an update

Gresik, Edward W.; Hosoi, Kazuo; Kurihara, Kinji; Maruyama, Shichiro; Ueha, T

doi:10.1076/ejom.34.3.221.13033

Cited by 34 publications

(27 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, it is well established that the cells of the GCT in the rodent submandibular gland are unique in their functions: the cells synthesize biologically potent proteins such as transforming growth factor á (TGF-á), hepatocyte growth factor, erythroid differentiation factor, endothelin, insulin-like growth factor (Gresik et al 1996), and four major enzymes of the tissue kallikrein family, three of which convert prorenin to renin andÏor arginyl renin (Hosoi et al 1998). In this context, CgA may be a newly identified constituent of biologically potent proteins stored in the cells of the GCT and secreted into saliva.…”

Section: Resultsmentioning

confidence: 99%

Salivary Secretion of Highly Concentrated Chromogranin a in Response to Noradrenaline and Acetylcholine in Isolated and Perfused Rat Submandibular Glands

Kanno

Asada

Yanase

et al. 1999

Experimental Physiology

View full text Add to dashboard Cite

SUMMARY Chromogranin A (CgA) is a member of a family of highly acidic proteins, chromogranins, which are co‐stored in the adrenergic neurons and paraneurons and co‐released with adrenaline and noradrenaline (NAd) in response to adequate stimulation. The present study provides novel evidence that CgA‐like immunoreactivity (IR) is stored in the exocrine cells in the granular convoluted tubule, and is secreted into saliva by stimulation with NAd and acetylcholine (ACh) in the isolated and perfused rat submandibular gland. NAd at 1 μM produced maximum secretion of CgA‐like IR (<< 0.9 mM) and a marked increase in salivary flow. Further increases in NAd concentration (10 or 100 μM) yielded concentration‐dependent decreases in both responses. ACh at 1 μM produced maximum salivary flow and a slight elevation of CgA‐like IR secretion (6 μM); 100 μM ACh decreased the salivary flow but increased the CgA‐like IR secretion (0.6 mM). Electron microscopic examination showed vigorous compound exocytosis of secretory granules in the cells of the granular convoluted tubule when the submandibular gland was stimulated with 1 μM NAd. These results provide an experimental basis for the view that the salivary CgA‐like IR secretion may be a sensitive and quantitative index of the activity of the sympathetic nervous system innervating the gland.

show abstract

Section: Resultsmentioning

confidence: 99%

Salivary Secretion of Highly Concentrated Chromogranin a in Response to Noradrenaline and Acetylcholine in Isolated and Perfused Rat Submandibular Glands

Kanno

Asada

Yanase

et al. 1999

Experimental Physiology

View full text Add to dashboard Cite

show abstract

“…The submandibular gland of rodents possesses a specialized segment of the duct system, called the granular convoluted tubule [Letic-Gavrilovic and Abe, 1990;Gresik et al, 1996;Sato and Miyoshi, 1998;Kurabuchi et al, 2001]. The granular convoluted tubule in rodents secretes somatostatin, epidermal growth factor, nerve growth factor, kallikreins and CgA-LI as a new member [Letic-Gavrilovic and Abe, 1990;Kanno et al, 1998].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Localization of Chromogranin A in the Acinar Cells of Equine Salivary Glands Contrasts with Rodent Glands

Sato

Kanno²,

Nagasawa³

et al. 2002

Cells Tissues Organs

View full text Add to dashboard Cite

We investigated the existence of chromogranin A (CgA) in salivary glands of the horse by Western blotting and enzyme immunoassay (EIA) using an antiserum against a peptide sequence of equine CgA. We also compared its cellular distribution between the horse and rat salivary glands with a tyramide signal amplification immunofluorescence technique. Western blotting gave three significant immunoreactive bands (74, 56 and 48 kDa) in adrenal medulla and three major salivary glands of horses. Immunoreactivities for CgA measured by EIA in horses were 154.05 ± 41.46, 20.32 ± 5.59 and 4.43 ± 2.23 pmol/g wet weight in the parotid gland, submandibular gland and sublingual gland, respectively, and 1.03 ± 0.407 pmol/mg protein in the saliva. Immunohistochemically, the positive reactivity was mainly recognized at acinar cells in equine salivary glands. This exhibits a contrast to the finding in the rat salivary glands that the CgA immunoreactivity is localized at the duct cells of the submandibular gland. These results provide novel evidence that in the horse, CgA is stored in the acinar cells of salivary glands, and secreted into saliva.

show abstract

“…Although many previous studies (reviewed in Gresik et al) (25,36) have shown that EGF protein and mRNA levels are correlated with each other and with the number of GCDs in rodents with experimentally reduced or absent GCD, or experimentally increased GCD, the findings of the current study indicate that the molecular mechanism controlling EGF secretion is independent of that controlling GCD growth. This idea regarding independent developmental and secretory regulatory pathways has been previously suggested by Siminoski et al (37), who proposed that submandibular NGF and EGF could be controlled by similar cellular or molecular mechanisms independent of the regulation of generalized growth responses.…”

Section: Figmentioning

confidence: 35%

“…These results may be explained by the ability of BCA to induce proliferation at small concentrations lower than 0.1 to 10 μM, whereas a similar proliferative capacity of estradiol is achieved at 0.2 or 0.3 μM on MCF7 cells in vitro (25)(26)(27)(28). Additionally, the results in the current study may be attributed to the effect of BCA and E2 on DNA synthesis.…”

Section: Discussionmentioning

confidence: 48%

See 1 more Smart Citation

Effect of Biochanin A versus 17β estradiol in rat submandibular salivary gland

2017

View full text Add to dashboard Cite

Abstract:The epigenetic nature of development mandates the observation of the effect of any exogenous substance, especially those with estrogenic activities, during critical phases of development. The submandibular gland (SMG) presents as a great model due to extensive postnatal development, and is known to be regulated and affected by hormones as well as growth factors. Herein, we observed postnatal development following low doses of Biochanin A (BCA) and 17β estradiol (E2) in rats. The pups were randomly divided into four groups: control, BCA, E2, and dimethyl sulfoxide (DMSO), and euthanized at the 6th, 15th, 30th, and 60th postnatal days (PND). SMG morphogenesis was assessed. The nuclear expression of estrogen receptor beta (ERβ) was evaluated immunohistochemically; ERβ expression was up-regulated by BCA and down-regulated by E2. Similarly, caspase three gene expression, assessed by real time polymerase chain reaction was increased in the BCA group but decreased in the E2 group. A significant decrease in epidermal growth factor gene expression was noted at PND 30. The results presented by this study provide evidence that the effect of a postnatal exposure of the SMG to Biochanin A during development could be linked to sex hormone-dependent disorders.

show abstract

The rodent granular convoluted tubule cell ? an update

Cited by 34 publications

References 0 publications

Salivary Secretion of Highly Concentrated Chromogranin a in Response to Noradrenaline and Acetylcholine in Isolated and Perfused Rat Submandibular Glands

Salivary Secretion of Highly Concentrated Chromogranin a in Response to Noradrenaline and Acetylcholine in Isolated and Perfused Rat Submandibular Glands

Immunohistochemical Localization of Chromogranin A in the Acinar Cells of Equine Salivary Glands Contrasts with Rodent Glands

Effect of Biochanin A versus 17β estradiol in rat submandibular salivary gland

Contact Info

Product

Resources

About