The role of antibiosis in the antagonism of different bacteria towards Helminthosporium solani, the causal agent of potato silver scurf

Martinez, Carole; Avis, Tyler J.; Simard, J.; Labonté, Jessica M.; Bélanger, Richard R.; Tweddell, Russell J.

doi:10.7202/013975ar

Cited by 17 publications

(15 citation statements)

References 28 publications

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“…This clearly suggested that antibiosis is an important contributor to the antagonistic effect of these bacteria through the diffusion of antifungal compounds into the medium. In confrontation with the present finding, Martinez et al [18] reported that plates containing exudates of precultured A. piechaudii, P. chlororaphis and S. griseus inhibited both mycelial growth and conidiation of Helminthosporium solani. Moreover, implication of diffusible bioactive metabolites such as phenazine, pyoluteorin, pyrrolnitrin and cyclic lipopeptides in antagonism is well documented [14].…”

Section: Assessment Of Inhibitory Traitssupporting

confidence: 76%

“…This may be attributed to the fact that broth-based environment allowed a synergistic expression of all the mechanisms involved in biocontrol from all the interacting sites. Concomitantly, variable responses in reduction in fungal biomass in broth culture as compared to dual plate assay have been reported as certain antifungal metabolites are induced or repressed by the absence or presence of pathogen [18]. In a similar study, Kumari and Khanna [16] reported 11.6-62.97% reduction in proliferation of F. oxysporum by Bacillus sp.…”

Section: Assessment Of Inhibitory Traitsmentioning

confidence: 98%

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Multifaceted Rhizobacteria-Mediated Growth Augmentation in Chickpea

2017

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Sixty-nine rhizobacteria isolated from chickpea rhizosphere were screened for their antagonistic potential against Fusarium oxysporum f. sp. ciceris under in vitro condition. Of these, 30 isolates inhibited growth of the pathogen and 13 potent antagonists were assessed for their functionality traits. Among the antagonistic traits exhibited by antagonists, production of diffusible antimetabolites insured its inhibitory effect on growth of test fungus as well as spore germination on solid media. Inhibition of fungus biomass in broth-based dual culture was revealed by 11 antagonists, while metabolic extracts of 5 isolates reduced the radial proliferation on solid media. Implication of biocidal volatiles in antagonism was demonstrated by 11 isolates; 3 produced cyanogenic volatiles, while all were ammonia producers. Investigation for hydrolytic activity demonstrated production of chitinase by 4 isolates and b-1,4-glucanase by 11, while all were protease and amylase producers. Revealing the dual potential of bioantagonists, all the isolates tested positive for IAA and salicylic acid production, 7 for siderophore production while 10 were P-solubilizers and 8 Zn-solubilizers. Under glass house condition, bacterization of chickpea seeds with potential isolate Ps14c alone and in consortium with Mesorhizobium ciceris reduced wilt incidence in pots (soil amended with F. oxysporum) to 48 and 28%, respectively, as compared to uninoculated control (74%) and also recorded appreciable increase in growth parameters. Present investigation clearly reveals the potential of rhizobacteria for use as bioinoculants.

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Section: Assessment Of Inhibitory Traitssupporting

confidence: 76%

Section: Assessment Of Inhibitory Traitsmentioning

confidence: 98%

Multifaceted Rhizobacteria-Mediated Growth Augmentation in Chickpea

2017

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“…The mechanisms which Pseudomonas use for effective biocontrol include direct antagonism, production of several metabolites with antimicrobial activity toward fungi and cell wall degrading enzymes (Haas and Keel 2003). Pseudomonas chlororaphis had previously been shown to produce antibiotics (Assis et al 1998;Chin-A-Woeng et al 1998), inhibited both mycelial growth and conidial germination of Helminthosporium solani (Martinez et al 2006) and also improved wheat growth under field conditions (Carlier et al 2008). In our previous work P. chlororaphis TSAU 13 had antagonistic effect on several pathogenic fungi such as F. oxysporum f.sp.…”

Section: Discussionmentioning

confidence: 96%

Pseudomonas chlororaphis: a salt-tolerant bacterial inoculant for plant growth stimulation under saline soil conditions

Egamberdieva

2011

Acta Physiol Plant

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Saline soils constitute a serious production problem for vegetable crops as they are known to suppress plant growth. One of the possible measures to improve crop health in such conditions is to use salt-tolerant bacterial inoculants which can control diseases and promote plant growth. In the present work the ability of Pseudomonas chlororaphis isolate TSAU13 to promote cucumber and tomato plant growth and to improve fruit yield by protecting these plants against soil-borne pathogens in salinated soil were investigated. The bacterial strain stimulated shoot growth (up to 32%), dry matter (up to 43%), and the fruit yield of tomato and cucumber (up to 16%) compared to the uninoculated control plants under saline conditions. The strain was able to survive on the root of 2-month-old plants. 29% of the cucumber and 27% of the tomato plants which had grown in soil to which no Fusarium solani spores had been added were diseased, whereas in the presence of the pathogenic fungus 58% of the cucumber and 52% tomato plants had disease symptoms. P. chlororaphis TSAU13 showed statistically significant disease reduction in comparison to the Fusarium-uninfected and infected control plants. Those results showed that P. chlororaphis TSAU13 has a great biotechnological potential in improvement of vegetable production in commercial greenhouses under saline conditions.

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“…Elad & Shtienberg (15) e Koné et al (18) observaram a presença de bactérias, actinobactérias e leveduras nos extratos aquosos supressivos e a perda da eficácia da supressividade sob microfiltração ou autoclavagem. A ação dos microrganismos presentes em extratos aquosos sobre a germinação de conídios de fungos pode estar associada à produção de sideróforos (14), metabólitos voláteis (21) e ou pelo mecanismo de antibiose (22). Todos os extratos aquosos e os materiais orgânicos, independentemente da autoclavagem, na concentração de 5% promoveram o crescimento micelial do patógeno (Tabelas 2, 3, 4).…”

Section: Discussão Dos Resultadosunclassified

Efeito de hidrolisado de peixe sobre o crescimento micelial e controle de Cylindrocladium spathiphylli em espatifilo

Visconti

Bettiol²,

Morandi³

2010

Summa phytopathol.

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Fish hydrolyzed (HP), poultry manure (CF), shrimp skin (CC), cattle manure (EB), sewage sludge (LE) and castorbean presscake (TM) were evaluated for their effect of aqueous extracts with and without autoclaving, on mycelial growth and conidial germination of Cylindrocladium spathiphylli. The effect of mixtures of residues with potting mixes and their volatile compounds were also evaluated on the mycelial growth of the pathogen. To evaluate the effect of HP in the suppressiveness to Cylindrocladium spathiphylli, HP was added in potting mix artificially infested, at concentrations of 0, 10, 20, 30, 40 and 50% of the volume of water required to reach the water retention capacity of the potting mix. The mixtures were incubated for 10 days and transferred to pots containing one plug of Spathiphyllum Opal per pot. In the experiments in vitro, aqueous extracts and mixtures containing HP showed the highest suppressiveness against the pathogen. In the Spathiphyllum growing, the suppressiveness occurred at concentrations higher than 20% of fish hidrolyzed.Cylindrocladium spathiphylli Schoulties, El-Gholl & Alfieri (teleomorfo: Calonectria spathiphylli El-Gholl, Uschida, Alfenas, Schub & Alfieri), agente causal da podridão do colo e da raiz de espatifilo (Spathiphyllum wallisi Regel), é o principal patógeno da cultura e ocorre em todos os países onde se cultiva essa ornamental (8,29). A cultura é conduzida basicamente em substratos à base de casca de pinus que são conducentes ao patógeno, acarretando perdas expressivas quando esse se instala no sistema de produção (8,24).O controle da doença é realizado principalmente com a aplicação de fungicidas à base de tiofanato metílico, iprodione, prochloraz e trifumizole (7) ao longo do ciclo da cultura que é de aproximadamente 18 meses. Porém, estes princípios ativos são efetivos somente quando aplicados em condições de baixa pressão da doença (24). As exigências da sociedade para a redução do uso de agrotóxicos associados aos problemas com resistência aos fungicidas (23) tem induzido a busca por métodos alternativos para o controle da doença (29). Assim, a introdução de agentes de biocontrole e a utilização de substratos desinfestados para o patógeno são estudados, porém, até o momento sem muito sucesso para o patossistema. Dessa forma, uma das alternativas é a utilização de resíduos orgânicos incorporados ao substrato padrão com o objetivo de induzir a supressividade ao patógeno (30).

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The role of antibiosis in the antagonism of different bacteria towards Helminthosporium solani, the causal agent of potato silver scurf

Cited by 17 publications

References 28 publications

Multifaceted Rhizobacteria-Mediated Growth Augmentation in Chickpea

Multifaceted Rhizobacteria-Mediated Growth Augmentation in Chickpea

Pseudomonas chlororaphis: a salt-tolerant bacterial inoculant for plant growth stimulation under saline soil conditions

Efeito de hidrolisado de peixe sobre o crescimento micelial e controle de Cylindrocladium spathiphylli em espatifilo

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