The Role of AtMUS81 in Interference-Insensitive Crossovers in A. thaliana

Berchowitz, Luke E.; Francis, Kirk E.; Bey, Alexandra L.; Copenhaver, Gregory P.

doi:10.1371/journal.pgen.0030132

Cited by 192 publications

(230 citation statements)

References 49 publications

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“…Thereafter, two genes, AtMSH4 and At-MER3/AtRCK, were identified as the components in class I crossovers [12,34,35]. Recently, AtMUS81 was also reported to be involved in interference-insensitive crossovers [36]. Our analysis of Atmsh5 meiocytes indicates that although the number of chiasmata is greatly reduced, there is still a subset of chiasmata remaining that are independent of AtMSH5.…”

Section: Discussionmentioning

confidence: 81%

The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis

Liu

et al. 2008

Cell Res

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npg MSH5, a member of the MutS homolog DNA mismatch repair protein family, has been shown to be required for proper homologous chromosome recombination in diverse organisms such as mouse, budding yeast and Caenorhabditis elegans. In this paper, we show that a mutant Arabidopsis plant carrying the putative disrupted AtMSH5 gene exhibits defects during meiotic division, producing a proportion of nonviable pollen grains and abnormal embryo sacs, and thereby leading to a decrease in fertility. AtMSH5 expression is confined to meiotic floral buds, which is consistent with a possible role during meiosis. Cytological analysis of male meiosis revealed the presence of numerous univalents from diplotene to metaphase I, which were associated with a great reduction in chiasma frequencies. The average number of residual chiasmata in the mutant is reduced to 2.54 per meiocyte, which accounts for ~25% of the amount in the wild type. Here, quantitative cytogenetical analysis reveals that the residual chiasmata in Atmsh5 mutants are randomly distributed among meiocytes, suggesting that AtMSH5 has an essential role during interferencesensitive chiasma formation. Taken together, the evidence indicates that AtMSH5 promotes homologous recombination through facilitating chiasma formation during prophase I in Arabidopsis.

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Section: Discussionmentioning

confidence: 81%

The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis

Liu

et al. 2008

Cell Res

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“…A minority of events form ZMM-independent JMs that are resolved as both COs and NCOs by the structure-selective nucleases (SSNs) Mus81-Mms4, Yen1, and Slx1-Slx4, which are responsible for most JM resolution during mitosis (Argueso et al, 2004;Santos et al, 2003;De Muyt et al, 2012;Ho et al, 2010;Muñoz-Galván et al, 2012;Zakharyevich et al, 2012; reviewed by Wyatt and West, 2014). A similar picture, with MutLg forming most meiotic COs and SSNs playing a minor role, is observed in several other eukaryotes (Berchowitz et al, 2007;Holloway et al, 2008;Plug et al, 1998).…”

Section: Introductionmentioning

confidence: 97%

Local chromosome context is a major determinant of crossover pathway biochemistry during budding yeast meiosis

Medhi¹,

Goldman

Lichten³

2016

Preprint

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The budding yeast genome contains regions where meiotic recombination initiates more frequently than in others. This pattern parallels enrichment for the meiotic chromosome axis proteins Hop1 and Red1. These proteins are important for Spo11-catalyzed double strand break formation; their contribution to crossover recombination remains undefined. Using the sequencespecific VMA1-derived endonuclease (VDE) to initiate recombination in meiosis, we show that chromosome structure influences the choice of proteins that resolve recombination intermediates to form crossovers. At a Hop1-enriched locus, most VDE-initiated crossovers, like most Spo11-initiated crossovers, required the meiosis-specific MutLg resolvase. In contrast, at a locus with lower Hop1 occupancy, most VDE-initiated crossovers were MutLg-independent. In pch2 mutants, the two loci displayed similar Hop1 occupancy levels, and VDE-induced crossovers were similarly MutLg-dependent. We suggest that meiotic and mitotic recombination pathways coexist within meiotic cells, and that features of meiotic chromosome structure determine whether one or the other predominates in different regions.

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“…The majority of meiotic COs are formed by the class I pathway, which ensures the obligate CO. Class I COs are sensitive to interference, a phenomenon whereby the occurrence of one CO reduces the probability of a second CO nearby. By contrast, class II COs are insensitive to interference and arise through different pathways (Berchowitz et al, 2007;Hollingsworth and Brill, 2004).…”

Section: Introductionmentioning

confidence: 99%

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

Chang

Xin

et al. 2013

The Plant Cell

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Replication protein A (RPA) is a conserved heterotrimeric protein complex comprising RPA1, RPA2, and RPA3 subunits involved in multiple DNA metabolism pathways attributable to its single-stranded DNA binding property. Unlike other species possessing a single RPA2 gene, rice (Oryza sativa) possesses three RPA2 paralogs, but their functions remain unclear. In this study, we identified RPA2c, a rice gene preferentially expressed during meiosis. A T-DNA insertional mutant (rpa2c) exhibited reduced bivalent formation, leading to chromosome nondisjunction. In rpa2c, chiasma frequency is reduced by ;78% compared with the wild type and is accompanied by loss of the obligate chiasma. The residual ;22% chiasmata fit a Poisson distribution, suggesting loss of crossover control. RPA2c colocalized with the meiotic cohesion subunit REC8 and the axis-associated protein PAIR2. Localization of REC8 was necessary for loading of RPA2c to the chromosomes. In addition, RPA2c partially colocalized with MER3 during late leptotene, thus indicating that RPA2c is required for class I crossover formation at a late stage of homologous recombination. Furthermore, we identified RPA1c, an RPA1 subunit with nearly overlapping distribution to RPA2c, required for ;79% of chiasmata formation. Our results demonstrate that an RPA complex comprising RPA2c and RPA1c is required to promote meiotic crossovers in rice.

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The Role of AtMUS81 in Interference-Insensitive Crossovers in A. thaliana

Cited by 192 publications

References 49 publications

The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis

The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis

Local chromosome context is a major determinant of crossover pathway biochemistry during budding yeast meiosis

Replication Protein A2c Coupled with Replication Protein A1c Regulates Crossover Formation during Meiosis in Rice

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