The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes

Frankič, Tamara; Pajk, T.; Rezar, Vida; Levart, Alenka; Salobir, Janez

doi:10.1016/j.fct.2006.06.002

Cited by 136 publications

(113 citation statements)

References 43 publications

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“…XPAC (fc: 11.279, DON5) is involved in nucleotide excision repair (Shimamoto et al, 1995). In male broiler chickens fed with 10 mg DON/kg feed, a comet assay was performed in spleen leukocytes, which detected aberrations, and a significantly higher amount of DNA breaks were found (Frankic et al, 2006). Interestingly, all the DNA repair-related genes mentioned above were only upregulated in group DON5 with the exception of RAD51L1, which was upregulated in DON2.5.…”

Section: Dna Repairmentioning

confidence: 99%

Fusarium mycotoxin-contaminated wheat containing deoxynivalenol alters the gene expression in the liver and the jejunum of broilers

Dietrich

Neuenschwander

Bucher

et al. 2012

Animal

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The effects of mycotoxins in the production of animal feed were investigated using broiler chickens. For the feeding trial, naturally Fusarium mycotoxin-contaminated wheat was used, which mainly contained deoxynivalenol (DON). The main effects of DON are reduction of the feed intake and reduced weight gain of broilers. At the molecular level, DON binds to the 60 S ribosomal subunit and subsequently inhibits protein synthesis at the translational level. However, little is known about other effects of DON, for example, at the transcriptional level. Therefore, a microarray analysis was performed, which allows the investigation of thousands of transcripts in one experiment. In the experiment, 20 broilers were separated into four groups of five broilers each at day 1 after hatching. The diets consisted of a control diet and three diets with calculated, moderate concentrations of 1.0, 2.5 and 5.0 mg DON/kg feed, which was attained by exchanging uncontaminated wheat with naturally mycotoxin-contaminated wheat up to the intended DON concentration. The broilers were held at standard conditions for 23 days. Three microarrays were used per group to determine the significant alterations of the gene expression in the liver (P , 0.05), and qPCR was performed on the liver and the jejunum to verify the results. No significant difference in BW, feed intake or feed conversion rate was observed. The nutrient uptake into the hepatic and jejunal cells seemed to be influenced by genes: SLC2A5 (fc: 21.54, DON2.5), which facilitates glucose and fructose transport and SLC7A10 (fc: 11.49, DON5), a transporter of D-serine and other neutral amino acids. In the jejunum, the palmitate transport might be altered by SLC27A4 (fc: 21.87, DON5) and monocarboxylates uptake by SLC16A1 (fc: 21.47, DON5). The alterations of the SLC gene expression may explain the reduced weight gain of broilers chronically exposed to DON-contaminated wheat. The decreased expressions of EIF2AK3 (fc: 21.29, DON2.5/5) and DNAJC3 (fc: 21.44, DON2.5) seem to be related to the translation inhibition. The binding of DON to the 60 S ribosomal subunit and the subsequent translation inhibition might be counterbalanced by the downregulation of EIF2AK3 and DNAJC3. The genes PARP1, MPG, EME1, XPAC, RIF1 and CHAF1B are mainly related to single-strand DNA modifications and showed an increased expression in the group with 5 mg DON/kg feed. The results indicate that significantly altered gene expression was already occurring at 2.5 mg DON/kg feed.

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Section: Dna Repairmentioning

confidence: 99%

Fusarium mycotoxin-contaminated wheat containing deoxynivalenol alters the gene expression in the liver and the jejunum of broilers

Dietrich

Neuenschwander

Bucher

et al. 2012

Animal

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“…There is increasing evidence that dietary nucleotides (NTs) affect different aspects of body function and health, including lipid metabolism, 1 immune function, [2][3][4][5] intestinal microbiota, 6 liver function and morphology 7 as well as growth performance. 8 Although nucleotides can be synthesized de novo, several tissues with a rapid turnover rate, such as the intestine, may benefit from an exogenous supply to save energy.…”

Section: Introduction Nmentioning

confidence: 99%

Developmental Effects of Dietary Nucleotides in Second-Generation Weaned Rats

et al. 2013

Journal of Medicinal Food

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The aim of the present study was to evaluate dietary effects of dietary nucleotides (NTs) on developmental parameters in second-generation Sprague-Dawley rats. Experimental design was set up as six groups, including one control and five nucleotide administered groups, doses ranged from 0.01% to 1.28% nucleotides. First-generation (F0) rats were divided into two terms: (1) fed for 90-day study; (2) mated for offspring (F1). After weaning, each group of second-generation F1 rats was fed the control diet. Evaluating parameters were weight gain and food utilization ratios of rats, hematological and biochemical parameters, and organ pathology. The results showed that there was a greater weight gain and food utilization ratio in weaned rats (F0 and F1). Furthermore, weight gain and food utilization ratios were increased in the first 2 weeks of F1 male and the first week of F1 female rats. There were no significant changes in blood indicators of NT groups with the exception of decrease in aspartate aminotransferase and serum uric acid levels. Overall, it was demonstrated that NT supplements could promote the early growth and development at a 0.01% dose. Although NTs may have cumulative reproductive effects, they were safe even at a high-dose supplementation. Additionally, NT supplementation could promote restoration and optimize liver function.

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“…Our DNA damage findings agree with the results of Awad et al (2012), who demonstrated that diets contaminated with the mycotoxin deoxynivalenol at moderate levels, in combination with low-protein feed, are able to induce lymphocyte DNA damage in chickens. Frankic et al (2006) also demonstrated DNA fragmentation in chicken splenocytes treated with T-2 toxin and deoxynivalenol.…”

Section: Discussionmentioning

confidence: 99%

In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

Zimmermann

Machado

Cadoná

et al. 2014

Rev. Bras. Cienc. Avic.

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The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B 1 (AFB 1 ) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB 1 concentrations in 5% CO 2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB 1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB 1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB 1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.

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The role of dietary nucleotides in reduction of DNA damage induced by T-2 toxin and deoxynivalenol in chicken leukocytes

Cited by 136 publications

References 43 publications

Fusarium mycotoxin-contaminated wheat containing deoxynivalenol alters the gene expression in the liver and the jejunum of broilers

Fusarium mycotoxin-contaminated wheat containing deoxynivalenol alters the gene expression in the liver and the jejunum of broilers

Developmental Effects of Dietary Nucleotides in Second-Generation Weaned Rats

In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

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