The role of dissolved oxygen and function of agitation in hyaluronic acid fermentation

Huang, Wei‐Chih; Chen, Shu‐Jen; Chen, Teh-Liang

doi:10.1016/j.bej.2006.10.011

Cited by 85 publications

(59 citation statements)

References 12 publications

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“…In the previous work [25], we studied the effects of DO level on the microbial HA production in chemostat culture of S. zooepidemicus. The results revealed that there existed a critical DO level of 5% and 10% of air saturation respectively for HA synthesis and cell growth, in accordance with what reported by Huang et al [24]. It was reported that the synthesis HA capsule could protect cells from the damage of oxygenderived free radicals [26].…”

Section: Resultssupporting

confidence: 91%

“…Effect of two-stage DO control approach on the microbial HA production Huang et al [24] found that DO played a role as a stimulant in HA synthesis and there existed a critical DO level of 5% of air saturation for HA synthesis. In the previous work [25], we studied the effects of DO level on the microbial HA production in chemostat culture of S. zooepidemicus.…”

Section: Resultsmentioning

confidence: 99%

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Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus

Liu

Chen

et al. 2009

Bioprocess Biosyst Eng

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Three different dissolved oxygen (DO) control approaches were proposed to improve hyaluronic acid (HA) production: a three-stage agitation speed control approach, a two-stage DO control approach, and an oxygen vector perfluorodecalin (PFC) applied approach. In the three-stage agitation speed control approach, agitation speed was 200 rpm during 0-8 h, 400 rpm during 8-12 h, and 600 rpm during 12-20 h. In the two-stage DO control strategy, DO was controlled at above 10% during 0-8 h and at 5% during 8-20 h. In the PFC applied approach, PFC (3% v/v) was added at 8 h. HA production reached 5.5 g/L in the three-stage agitation speed control culture model, and 6.3 g/L in two-stage DO control culture model, and 6.6 g/L in the PFC applied culture model. Compared with the other two DO control approaches, the PFC applied approach had a lower shear stress and thus a higher HA production was achieved.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus

Liu

Chen

et al. 2009

Bioprocess Biosyst Eng

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“…Since the precursors, UDP-glucuronic acid (UDP-GlcUA) and UDP-N-acetyl glucosamine (UDP-GlcNAc), are side reactions of the glycolytic pathway beginning from glucose-6-phosphate to fructose-6-phosphate, respectively [25], especially, glucose was used frequently for HA fermentation by Streptococcus sp. [1,7,14,16,18,26,29]. In our study, glucose was also determined to be the best carbon source for HA production by Streptococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Optimization of medium components for high-molecular-weight hyaluronic acid production by Streptococcus sp. ID9102 via a statistical approach

Song

Kang

et al. 2009

J Ind Microbiol Biotechnol

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Hyaluronic acid (HA), linear high-molecular-weight glycosaminoglycan produced from Streptococcus sp., has raised interest in the medical and cosmetics industries because of the various biological functions of HA. In this paper, we report on the optimization of medium components for HA production in Streptococcus sp. ID9102 (KCTC 11935BP) by two-step optimization (one-factor-at-a-time and taguchi orthogonal array design). In the first step, medium components, such as carbon, nitrogen, phosphate, and mineral sources, were selected for HA production in Streptococcus sp. ID9102 (KCTC 11935BP) using the one-factor-at-a-time method. In the second step, the concentration of the selected medium components was optimized using taguchi orthogonal array design. The design for medium optimization was developed and analyzed using MINITAB 14 software. In addition, the effect of amino acid and organic acid, such as glutamine, glutamate, and oxalic acid, was studied for HA production in Streptococcus sp. ID9102 (KCTC 11935BP). Through these processes, the optimum medium comprising 4% glucose, 0.75% yeast extract, 1.0% casein peptone, 0.25% K(2)HPO(4), 0.05% MgCl(2), 0.5% NaCl, 0.04% glutamine, 0.06% glutamate, and 0.02% oxalic acid was determined. We were able to produce HA with a molecular weight of 5.9 x 10(6) at a productivity of 6.94 g/l on pilot scale fermentation.

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“…The concentration of dissolved oxygen is known to aVect the expression of respiratory enzymes such as dehydrogenases and oxidases in some microorganisms [28], and these changes in turn inXuence the production of metabolites such as organic acids [16], biopolymers [29] and polysaccharides [17]. The supply of dissolved oxygen to a fermentation is strongly aVected by the aeration rate and agitation speed of the impeller [4,7].…”

Section: Introductionmentioning

confidence: 99%

Production of l-phenylalanine from glycerol by a recombinant Escherichia coli

Khamduang

Packdibamrung

Chutmanop

et al. 2009

J Ind Microbiol Biotechnol

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The production of L-phenylalanine is conventionally carried out by fermentations that use glucose or sucrose as the carbon source. This work reports on the use of glycerol as an inexpensive and abundant sole carbon source for producing L-phenylalanine using the genetically modiWed bacterium Escherichia coli BL21(DE3). Fermentations were carried out at 37°C, pH 7.4, using a deWned medium in a stirred tank bioreactor at various intensities of impeller agitation speeds (300-500 rpm corresponding to 0.97-1.62 m s ¡1 impeller tip speed) and aeration rates (2-8 L min ¡1 , or 1-4 vvm). This highly aerobic fermentation required a good supply of oxygen, but intense agitation (impeller tip speed »1.62 m s ¡1 ) reduced the biomass and L-phenylalanine productivity, possibly because of shear sensitivity of the recombinant bacterium. Production of L-phenylalanine was apparently strongly associated with growth. Under the best operating conditions (1.30 m s ¡1 impeller tip speed, 4 vvm aeration rate), the yield of L-phenylalanine on glycerol was 0.58 g g ¡1 , or more than twice the best yield attainable on sucrose (0.25 g g ¡1 ). In the best case, the peak concentration of L-phenylalanine was 5.6 g L ¡1 , or comparable to values attained in batch fermentations that use glucose or sucrose. The use of glycerol for the commercial production of L-phenylalanine with E. coli BL21(DE3) has the potential to substantially reduce the cost of production compared to sucrose-and glucosebased fermentations.

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The role of dissolved oxygen and function of agitation in hyaluronic acid fermentation

Cited by 85 publications

References 12 publications

Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus

Comparative study on the influence of dissolved oxygen control approaches on the microbial hyaluronic acid production of Streptococcus zooepidemicus

Optimization of medium components for high-molecular-weight hyaluronic acid production by Streptococcus sp. ID9102 via a statistical approach

Production of l-phenylalanine from glycerol by a recombinant Escherichia coli

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