The role of lipopolysaccharide in infectious bone resorption of periapical lesion

Hong, Chuang‐Ye; Lin, Sze‐Kwan; Kok, Sang‐Heng; Cheng, Shih‐Jung; Lee, Ming‐Shu; Wang, Tong-Mei; Chen, Chuan-Shuo; Lin, Li‐Deh; Wang, Juo-Song

doi:10.1111/j.0904-2512.2004.00045.x

Cited by 139 publications

(152 citation statements)

References 32 publications

Supporting

Mentioning

120

Contrasting

Unclassified

Order By: Relevance

“…2E). LPS is well known to elicit TNF-α (Abu-Amer et al 1997;Zou and Bar-Shavit 2002;Itoh et al 2003), IL-6 (Itoh et al 2003;Sato et al 2004), IL-1α (Hong et al 2004), andIL-1β (Itoh et al 2003) release from monocytes. It is also well established that these cytokines promote Oc formation (Jimi et al 1999;Azuma et al 2000;Zhang et al 2001;Zou et al 2001;Kudo et al 2003;Wei et al 2005).…”

Section: Figmentioning

confidence: 99%

Lipopolysaccharide-Induced Osteoclastogenesis from Mononuclear Precursors: A Mechanism for Osteolysis in Chronic Otitis

Nason

Jung

Chole

2009

JARO

View full text Add to dashboard Cite

Osteoclasts are the only cells capable of carrying out bone resorption and therefore are responsible for the osteolysis seen in infectious diseases such as chronic otitis media and infected cholesteatoma. Pseudomonas aeruginosa is the most common organism isolated from these infectious middle ear diseases. In this study, we examined the mechanisms by which P. aeruginosa lipopolysaccharide (LPS) stimulates osteoclastogenesis directly from mononuclear osteoclast precursor cells. Osteoclast precursors demonstrated robust, bone-resorbing osteoclast formation when stimulated by P. aeruginosa LPS only if previously primed with permissive, sub-osteoclastogenic doses of receptor activator of NF-κB ligand (RANKL), suggesting that LPS is osteoclastogenic only during a specific developmental window. Numerous LPS-elicited cytokines were found to be released by osteoclast precursors undergoing P. aeruginosa LPS-mediated osteoclast formation. Two lines of evidence suggest that several cytokines promote Oc formation in an autocrine/paracrine manner. First, inhibition of several cytokine pathways including TNF-α, IL-1, and IL-6 block the osteoclastogenesis induced by LPS. Secondly, increased expression of the receptors for TNF-α and IL-1 was demonstrated by real-time quantitative polymerase chain reaction. Such a mechanism has not previously been established and demonstrates the ability of osteoclast precursors to autonomously facilitate bone destruction.

show abstract

Section: Figmentioning

confidence: 99%

Lipopolysaccharide-Induced Osteoclastogenesis from Mononuclear Precursors: A Mechanism for Osteolysis in Chronic Otitis

Nason

Jung

Chole

2009

JARO

View full text Add to dashboard Cite

show abstract

“…Many kinds of cytokines are released during dental pulp inflammation, including TNF-α, IL-1 and TGF-β1 [1][2][3] . These and TIMP-1, -2 and -3 [13][14][15][16][17][18] .…”

Section: Discussionmentioning

confidence: 99%

“…Dental caries often causes pulp inflammation once it reaches the dentine. In deep caries, there are many kinds of bacterial components, such as lipopolysaccharide (LPS) from gram-negative bacteria, that induce the production of proinflammatory mediators, including tumor necrosis factor (TNF)-α, interleukin (IL)-1 and transforming growth factor (TGF)-β1 [1][2][3] .…”

Section: Introductionmentioning

confidence: 99%

TNF-^|^alpha; and LPS Enhance MMP Production in Human Dental Pulp Cells of Deciduous Teeth

Kawai

Harada

Daito

et al. 2012

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Dental caries often causes pulp inflammation once it reaches the dentine. In deep caries, there are many kinds of bacterial components, such as lipopolysaccharide (LPS), that induce the production of proinflammatory mediators, including tumor necrosis factor (TNF)-α. Inflammation in dental pulp tissue is associated with tissue degradation, and matrix metalloproteinases (MMPs) are believed to participate in this destruction. In the present study, we examined whether TNF-α and LPS affect the production of MMP-1 and MMP-3 in human dental pulp cells (HDPCs) of deciduous teeth utilizing RT-PCR and ELISA analyses. Exposure of HDPCs to TNF-α at 10 ng/ml for 6 h significantly increased the gene expression of MMP-1 and MMP-3. MMP-1 gene expression was slightly affected by exposure to LPS, while MMP-3 gene expression was significantly elevated by LPS at 0.1, 1 and 5 µg/ml. TNF-α at 10 ng/ml and LPS at 1 and 5 µg/ml potentiated the protein production of MMP-3, but not MMP-1, in HDPCs. The results of this study suggests that the mechanisms by which TNF-α and LPS induce MMP-3 expression may be important links in the pathogenesis of inflammatory diseases and reparative dentin formation in HDPCs of deciduous teeth.

show abstract

“…1,2,3 LPS is released during multiplication or death of gram-negative bacteria, and is associated with many biological effects, such as release of pro-inflammatory mediators 4 and induction of periapical bone resorption. 5 The endotoxins can adhere to mineralized tissues 6 and disseminate through the dentinal tubules, 7 making root canal sterilization difficult if only chemo-mechanical preparation is used. 8 Although the effects of different disinfection strategies 9,10,11,12 on LPS have already been examined, none of them are fully effective as yet.…”

Section: Introductionmentioning

confidence: 99%

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

et al. 2016

View full text Add to dashboard Cite

The present study aims to verify the effect of ozone gas (OZY ® System) and high frequency electric pulse (Endox ® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY ® System, one 120-second-pulse (OZY 1p); OZY ® System, four 24-second-pulses (OZY 4p); and Endox ® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.

show abstract

The role of lipopolysaccharide in infectious bone resorption of periapical lesion

Abstract: It is suggested that LPS released from the infected root canal triggers the synthesis of IL-1 alpha and TNF-alpha from macrophages. These pro-inflammatory cytokines up-regulate the production of MMP-1 by macrophages to promote periapical bone resorption.

Cited by 139 publications

References 32 publications

Lipopolysaccharide-Induced Osteoclastogenesis from Mononuclear Precursors: A Mechanism for Osteolysis in Chronic Otitis

Lipopolysaccharide-Induced Osteoclastogenesis from Mononuclear Precursors: A Mechanism for Osteolysis in Chronic Otitis

TNF-^|^alpha; and LPS Enhance MMP Production in Human Dental Pulp Cells of Deciduous Teeth

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

Contact Info

Product

Resources

About