The role of metalloproteinases in the development of primary open-angle glaucoma

Svinareva, D.I.; Чурносов, М И

doi:10.17116/oftalma2020136041146

Cited by 6 publications

(1 citation statement)

References 23 publications

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“…The cytokine TGF-beta 1 influences the activation of MMPs [38]. Conversely, it has been posited that MMP-9 contributes to TGF-beta activation by releasing it from the matrix [39].…”

Section: Discussionmentioning

confidence: 99%

New Biomarker Combination Related to Oxidative Stress and Inflammation in Primary Open-Angle Glaucoma

Dammak

Naves²,

Huete-Toral

et al. 2023

Life

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Glaucoma is a multifactorial neurodegenerative disease and the second leading cause of blindness. Detection of clinically relevant biomarkers would aid better diagnoses and monitoring during treatment. In glaucoma, the protein composition of aqueous humor (AH) is relevant for the discovery of biomarkers. This study analyzes AH protein concentrations of putative biomarkers in patients with primary open-angle glaucoma (POAG) compared to a control group. Biomarkers were selected from known oxidative-stress and inflammatory pathways. Osteopontin (OPN), matrix metalloproteinase 9 (MMP-9), tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-beta (TGF-beta), and interleukin-10 (IL-10) were measured using the ELISA technique. Thirty-two patients were recruited to the study, including sixteen control and sixteen glaucoma patients. The glaucoma group consisted of patients diagnosed with glaucoma. In both groups, the aqueous humor sample was obtained during cataract surgery. A significant increase in OPN, MMP-9, TNF-alpha, and IL-10 was observed in the POAG aqueous humor, compared to the control group (p < 0.05). Of note, the AH of POAG patients contained 5.6 ± 1.2-fold more OPN compared to that of control patients. Different expression profiles of oxidative stress-related and inflammatory biomarkers were observed between patients with POAG and controls. This confirms the reported involvement of inflammatory and oxidative stress pathways in POAG pathophysiology. In the future, several, targeted AH proteins may be used to generate a potential biomarker expression profile of this disease, aiding diagnoses and disease progression monitoring. This approach highlights the importance of biomarkers in the future. Biomarkers provide a way to measure disease progression and response to treatment. In the future, biomarkers will play a more critical role in the toolkit of ophthalmology healthcare professionals as the field moves towards personalized medicine and precision healthcare.

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“…The cytokine TGF-beta 1 influences the activation of MMPs [38]. Conversely, it has been posited that MMP-9 contributes to TGF-beta activation by releasing it from the matrix [39].…”

Section: Discussionmentioning

confidence: 99%

New Biomarker Combination Related to Oxidative Stress and Inflammation in Primary Open-Angle Glaucoma

Dammak

Naves²,

Huete-Toral

et al. 2023

Life

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Matrix Metalloproteinases in Glaucoma: An Updated Overview

Zhang

Han

et al. 2023

Seminars in Ophthalmology

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Analysis of the functional role of polymorphism in the CDKN2B-AS1 gene GWAS-significant for primary open-angle glaucoma (an in-silico study)

2021

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Первичная открытоугольная глаукома (ПОУГ) -самая распространенная форма глаукомы, в развитии которой значимую роль играют генетические факторы. По данным полногеномных исследований (Genome Wide Association Studies, GWAS), с ПОУГ ассоциирован ген CDKN2B-AS1. Цель исследования. Изучить in silico функциональное значение GWAS-значимого для первичной открытоугольной глаукомы полиморфизма гена CDKN2B-AS1. Материал и методы. Для анализа in silico на основе данных GWAS-каталога были отобраны пять полиморфных локусов гена CDKN2B-AS1 (rs1063192, rs7865618, rs2157719, rs944800, rs4977756), ассоциированных с ПОУГ. Изучены регуляторный потенциал, связь с экспрессией и альтернативным сплайсингом генов полиморфизма гена CDKN2B-AS1. В работе использовались мировые базы данных по функциональной геномике -HaploReg и GTExportal. Результаты. Выявлено важное функциональное значение полиморфных локусов rs1063192, rs7865618, rs2157719, rs944800, rs4977756 гена CDKN2B-AS1. Данные локусы находятся в области гистонов, маркирующих энхансеры в регионе гиперчувствительности к ДНКазе I более чем в десяти различных органах и тканях, в области регуляторных мотивов ДНК к пяти факторам транскрипции (AIRE, GATA, Tgif1, Pou2f2 и Zfp187), ассоциированы с экспрессией трех генов (CDKN2B-AS1, CD-KN2B, CDKN2A) и альтернативным сплайсингом транскриптов двух генов (CDKN2B-AS1 и RP11-149I2.4) в патогенетически значимых для формирования глаукомы культурах клеток, органах, тканях. Заключение. Полиморфизм гена CDKN2B-AS1 (rs1063192, rs7865618, rs2157719, rs944800, rs4977756) имеет важное функциональное значение -связан с экспрессией и альтернативным сплайсингом генов, имеет значимый регуляторный потенциал, что может лежать в основе его ассоциации с ПОУГ.

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The role of metalloproteinases in the development of primary open-angle glaucoma

Cited by 6 publications

References 23 publications

New Biomarker Combination Related to Oxidative Stress and Inflammation in Primary Open-Angle Glaucoma

New Biomarker Combination Related to Oxidative Stress and Inflammation in Primary Open-Angle Glaucoma

Matrix Metalloproteinases in Glaucoma: An Updated Overview

Analysis of the functional role of polymorphism in the CDKN2B-AS1 gene GWAS-significant for primary open-angle glaucoma (an in-silico study)

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