The role of miR‐29 family in disease

Horita, Masahiro; Farquharson, Colin; Stephen, Louise A.

doi:10.1002/jcb.29896

Cited by 69 publications

(57 citation statements)

References 246 publications

(320 reference statements)

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“…Our data showed that miR-29c deletion induced bone mass loss may be not attributed to adipose differentiation. References identify that miR-29c induced bone loss results from the dysfunction of osteoblast and osteoclast differentiation (Horita et al, 2021), which is consistent with our work.…”

Section: Discussionsupporting

confidence: 93%

miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone

Ouyang

Sun

et al. 2022

iScience

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Coordination between osteogenesis and angiogenesis is required for bone homeostasis. Here, we show that miR-29cb2 is a bone-specific miRNA and plays critical roles on angiogenesis-osteogenesis coupling during bone remodeling. Mice with deletion of miR-29cb2 exhibit osteopenic phenotypes and osteoblast impairment, accompanied by pronounced decreases in specific H vessels. The decrease in bone miR-29cb2 was associated with pathological ovariectomy stimuli. Mechanistically, hypoxia-inducible factor (HIF)-3a, as a target for miR-29cb2, inhibits HIF-1a activity by competitively bonding with HIF-1b. Notably, miR-29cb2 in peripheral blood (PB) nearly is undetectable in sham and significantly increases in ovariectomy mice. Further evaluation from osteoporosis patients demonstrates similar signatures. ROC analysis shows miR-29cb2 in PB has higher sensitivity and specificity for diagnosing osteoporosis when compared with four clinical biomarkers. Collectively, these findings reveal that miR-29cb2 is essential for bone remodeling by inhibiting HIF-3a and elevated bone-specific miR-29cb2 in PB, which may be a promising biomarker for bone loss

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Section: Discussionsupporting

confidence: 93%

miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone

Ouyang

Sun

et al. 2022

iScience

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“…Deeper knowledge about the pathophysiological role of CPXM2 in the development of cardiac hypertrophy and fibrosis could help us to implement novel targeted therapies for these conditions. MiRNAs, in general, have been shown to act as potential therapeutic agents [66,67]. For example, Ban et al demonstrated miRNA-497 to accelerate wound healing in diabetic mice after local injection [68].…”

Section: Cpxm2 Expression Is Directly Regulated Via Mir-29b and Via M...mentioning

confidence: 99%

MiRNA-29b and miRNA-497 Modulate the Expression of Carboxypeptidase X Member 2, a Candidate Gene Associated with Left Ventricular Hypertrophy

Subrova

Böhme

Gillespie

et al. 2022

IJMS

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Left ventricular hypertrophy (LVH) is a major risk factor for adverse cardiovascular events. Recently, a novel candidate gene encoding the carboxypeptidase X member 2 (CPXM2) was found to be associated with hypertension-induced LVH. CPXM2 belongs to the M14 family of metallocarboxypeptidases, yet it lacks detectable enzyme activity, and its function remains unknown. Here, we investigated the impact of micro (mi)RNA-29b, miRNA-195, and miRNA-497 on the posttranscriptional expression control of CPXM2. Candidate miRNAs for CPXM2 expression control were identified in silico. CPXM2 expression in rat cardiomyocytes (H9C2) was characterized via real-time PCR, Western blotting, and immunofluorescence. Direct miRNA/target mRNA interaction was analysed by dual luciferase assay. CPXM2 was expressed in H9C2 and co-localised with z-disc associated protein PDZ and LIM domain 3 (Pdlim3). Transfection of H9C2 with miRNA-29b, miRNA-195, and miRNA-497 led to decreased levels of CPXM2 mRNA and protein, respectively. Results of dual luciferase assays revealed that miRNA-29b and miRNA-497, but not miRNA-195, directly regulated CPXM2 expression on a posttranscriptional level via binding to the 3′UTR of CPXM2 mRNA. We identified two miRNAs capable of the direct posttranscriptional expression control of CPXM2 expression in rat cardiomyocytes. This novel data may help to shed more light on the—so far—widely unexplored expression control of CPXM2 and its potential role in LVH.

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“…The miRNA-29 family includes miRNA-29a, miRNA-29b-1, miRNA-29b-2, and miRNA-29c encoded by genes located on chromosome 7 (7q32.3) and on chromosome 1 (1q32.2). The miRNA-29 family controls cell apoptosis, proliferation, and differentiation and exerts an antifibrotic activity targeting genes concerned extracellular matrix remodelling [39].…”

Section: Mirna-29mentioning

confidence: 99%

TFEB Signalling-Related MicroRNAs and Autophagy

2021

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The oncogenic Transcription Factor EB (TFEB), a member of MITF-TFE family, is known to be the most important regulator of the transcription of genes responsible for the control of lysosomal biogenesis and functions, autophagy, and vesicles flux. TFEB activation occurs in response to stress factors such as nutrient and growth factor deficiency, hypoxia, lysosomal stress, and mitochondrial damage. To reach the final functional status, TFEB is regulated in multimodal ways, including transcriptional rate, post-transcriptional regulation, and post-translational modifications. Post-transcriptional regulation is in part mediated by miRNAs. miRNAs have been linked to many cellular processes involved both in physiology and pathology, such as cell migration, proliferation, differentiation, and apoptosis. miRNAs also play a significant role in autophagy, which exerts a crucial role in cell behaviour during stress or survival responses. In particular, several miRNAs directly recognise TFEB transcript or indirectly regulate its function by targeting accessory molecules or enzymes involved in its post-translational modifications. Moreover, the transcriptional programs triggered by TFEB may be influenced by the miRNA-mediated regulation of TFEB targets. Finally, recent important studies indicate that the transcription of many miRNAs is regulated by TFEB itself. In this review, we describe the interplay between miRNAs with TFEB and focus on how these types of crosstalk affect TFEB activation and cellular functions.

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The role of miR‐29 family in disease

Cited by 69 publications

References 246 publications

miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone

miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone

MiRNA-29b and miRNA-497 Modulate the Expression of Carboxypeptidase X Member 2, a Candidate Gene Associated with Left Ventricular Hypertrophy

TFEB Signalling-Related MicroRNAs and Autophagy

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