The screening of phytochemical and antioxidant activity of agarwood leaves (Aquilaria malaccensis) from two sites in North Sumatra, Indonesia

Abstract

Abstract. Batubara R, Surjanto, Hanum TI, Handika A, Afandi O. 2020. Phytochemical screening and antioxidant activity of agarwood leaves (Aquilaria malaccensis) from two sites in North Sumatra, Indonesia. Biodiversitas 21: 1588-1596. Agarwood of gaharu (Aquilaria malaccensis Lamk) has an antioxidant activity which can reduce free radicals. This research was conducted to analyze the chemical compounds of agarwood, and the antioxidant activities from two different grown sites, Laru, and Hutanabolon Village. Ethanol extracts of the agarwood leaves (EEAL) were obtained through maceration method. The phytochemical screening included the examination of secondary plant metabolites, while antioxidant activity was determined by free radicals scavenging activity against 1,1- diphenyl-2-picrylhydrazyl (DPPH). An observable parameter was the percentage of free-radical-reduction with different concentrations (40, 60, 80 and 100 ppm) and inhibitory concentrations (IC50) value by using regression equation. The phytochemical screening result showed that simplicia and EEAL from Laru grown site contained secondary plant metabolites, such as steroid/triterpenoids, flavonoids, saponins, and tannins, while simplicia leaves of agarwood from Hutanabolon grown site contained alkaloids, steroid/triterpenoids, saponins, and tannins, However, EEAL from Hutanabolon was found to only contain alkaloids. The f antioxidant activity of EEAL from Laru 27.887 ± 0.560 ppm was not different from Hutanabolon 27.807 ± 4.630 ppm. The EEAL from both Laru and Hutanabolon had a very strong antioxidant activity.

Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties

“…The results of Py GC-MS analysis also support the antioxidant analysis results since the major compounds contained in both the young and the mature leaves were identified as acid compounds, namely Octadecanoic acid (CAS) Stearic acid. Batubara et al (2020) noted that the primary compound with the highest concentration in agarwood (A. malaccensis) leaves from Laru Village is also Octadecanoic acid (CAS) Stearic acid, contributing to the great role in strong antioxidant capacity of agarwood leaves.…”

A Review: Aquilaria Species as Potential of Pharmacological

“…This type of tree is found in many countries in the oriental region such as Indonesia, Malaysia, Thailand, India, Laos, and China [8]. In addition, Aquilaria extract has also been reported to have anticancer activity [9,11,14], antiinflammatory [1], antioxidant [3,9,14], and antidiabetic [14] which have been demonstrated in many in vivo and in vitro models. Therefore, the pharmacological effects of Aquilaria with different species and tree parts have different and similar pharmacological properties.…”

Strategi Pengembangan Model Bisnis Teh Gaharu CV.Barokah Access, Sambas, Kalimantan Barat

“…Selama menunggu panen gubal, petani dapat mengolah daun gaharu menjadi beberapa produk minuman fungsional. Daun gaharu mengandung antioksidan yang dapat dapat mengurangi radikal bebas dan meningkatkan daya tahan tubuh (Simatupang et al 2015 ;Adrianar et al 2015;Nasution et al 2015;Batubara et al 2020). Daun gaharu mengandung alkaloid, flavonoid, triterpenoid, dan tanin.…”

“…Kandungan tanin berkisar antara 4,95% -5,62% (Batubara et al 2018). Ekstrak daun gaharu menggunakan etanol dihasilkan aktivitas antioksidan daun gaharu 27.887 ± 0,560 ppm (Batubara, et al 2020). Sifat farmakologis daun gaharu lainnya adalah analgesik, anti-rematik, antiinflamasi, antikanker, antitumor, antioksidan, antibakteri, antijamur, antidiabetik, antihistamin, penurun lipid, pencahar, penghambatan lipid, asetilkolinesterase (AChE) dan hepatoprotektif (Adam et al 2017).…”

“…• Kandungan Anti oksidan produk Beberapa hasil penelitian membuktikan bahwa daun gaharu mengandung antioksidan yang dapat dapat mengurangi radikal bebas dan meningkatkan daya tahan tubuh. Ekstrak daun gaharu menggunakan etanol dihasilkan aktivitas antioksidan daun gaharu 27.887 ± 0,560 ppm(Batubara, et al 2020). Sifat farmakologis daun gaharu lainnya adalah analgesik, antirematik, antiinflamasi, antikanker, antitumor, antioksidan, antibakteri, antijamur, antidiabetik, antihistamin, penurun lipid, pencahar, penghambatan lipid, asetilkolinesterase (AChE) dan hepatoprotektif (Adam et al 2017).• Kearifan lokal dan minuman kesehatanTeh daun gaharu telah lama dikonsumsi oleh masyarakat lokal karena dipercaya dapat meningkatkan stamina dan daya tahan tubuh dari berbagai penyakit.…”