Relative potency of eight compounds (four pairs of similar types) to adults and old larvae of three species of cyclorhaphous diptera was determined by injection, oral or topical application. The following points emerged from comparisons of equitoxic doses: (a) C. putoria was consistently more susceptible than L. sericata. M. domestica was more susceptible to organochlorines than either of the blowflies, but less susceptible to organophosphorus, carbamate or pyrethroid insecticides; (b) the most potent insecticides (bioresmethrin, diazinon) were about equally toxic to adults and larvae of all species; but some compounds (especially DDT, gamma‐BHC and propoxur) were relatively much less effective against larvae; (c) LD50 values for oral treatments were higher than by injection, and those for contact treatments higher still. These “penetration factors” were highly correlated within each of the stages. This could be due to similarity in the cuticular barrier and (or) detoxication; (d) experiments with various synergistic compounds showed higher synergistic factors for larvae than adults (with DDT, bioresmethrin and propoxur). This probably indicates a more efficient detoxication system in the larvae, which is consistent with findings (b) and (c).