The Sensitivity of Grivet Monkey Kidney Cell Line Bs-C-1 for Propagation And Isolation of Certain Human Viruses

Schmidt, Nathalie J.; Lennette, Edwin H.; Shon, Carol W.; Dennis, Juanita

doi:10.2105/ajph.54.9.1522

Cited by 11 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On cytogenetic analysis, HL-8 exhibited a consistent degree of aneuploidy with a diploid mean chromosome number in all passages. Schmidt and colleagues (10) noted this type of aneuploidy in a continuous green monkey kidney cell line, BS-C-1. Unlike HL-8, which died at passage 50 BS-C-1 developed immortality and by passage 75 had obvious neoplastic-like morphologic changes and a polyploid chromosomal configuration.…”

mentioning

confidence: 97%

“…There have been only two published reports of diploid kidney strains, both of human fetal origin (.3, 11). Kidney lines have been universally less sensitive to human viruses than primary rhesus MK (1,2,8,9,10). Although a green monkey line, BS-C-1, (10) and a diploid human fetal kidney strain, HFDK (1 l ) , are as sensitive as primary rhesus MK to enteroviruses, these and all other previous kidney lines are unsatisfactory for growth of influenza A2, parainfluenza 1, 3, and 4, adenoviruses and herpes simplex virus (2,8,9,10,11).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Biological Characteristics and Viral Spectrum of Serially Cultivated Fetal Rhesus Monkey Kidney Cells

Forman

Inhorn

Sheaff

et al. 1969

Experimental Biology and Medicine

View full text Add to dashboard Cite

mentioning

confidence: 97%

mentioning

confidence: 99%

Biological Characteristics and Viral Spectrum of Serially Cultivated Fetal Rhesus Monkey Kidney Cells

Forman

Inhorn

Sheaff

et al. 1969

Experimental Biology and Medicine

View full text Add to dashboard Cite

“…The Vero analysis was consistent with homozygosity for the AGM clone 2 CCR5 sequence. In contrast, sequencing analysis of CCR5 amplified from BS-C-1 cells, which were derived from a grivet AGM (40), suggested that this cell line is heterozygous in the coding sequence of its genomic DNA for a novel point mutation which encodes a Q93K substitution in the AGM clone 2 CCR5 protein. Thus, among the five AGM DNA samples that we examined, four appeared to have derived from CCR5 heterozygotes and all of the amino acid changes were distinct.…”

Section: Discussionmentioning

confidence: 91%

Polymorphisms in the CCR5 genes of African green monkeys and mice implicate specific amino acids in infections by simian and human immunodeficiency viruses

Kuhmann

Platt

Kozak

et al. 1997

J Virol

View full text Add to dashboard Cite

CCR5, a receptor for the CC chemokines RANTES, Mip1␣, and Mip1␤, has been identified as a coreceptor for infections by macrophage-tropic isolates of human immunodeficiency virus type 1 (HIV-1). To study its structure and function, we isolated cDNA clones of human, African green monkey (AGM), and NIH/Swiss mouse CCR5s, and we quantitatively analyzed infections by macrophage-tropic HIV-1 and SIV mac251 after transfecting human HeLa-CD4 cells with the CCR5 expression vectors. The AGM and NIH/Swiss mouse CCR5 proteins are 97.7 to 98.3% and 79.8% identical to the human protein, respectively. In addition, we analyzed site-directed mutants and chimeras of these CCR5s. Cell surface expression of CCR5 proteins was monitored by using a specific rabbit antiserum and by binding the chemokine [ 125 I]Mip1␤. Our major results were as follows. (i) Two distinct AGM CCR5 sequences were reproducibly found in DNA from CV-1 cells. The AGM clone 1 CCR5 protein differs from that of clone 2 by two substitutions, Y14N in the amino-terminal extracellular region and L352F at the carboxyl terminus. Interestingly, AGM clone 1 CCR5 was inactive as a coreceptor for all tested macrophage-tropic isolates of HIV-1, whereas AGM clone 2 CCR5 was active. As shown by chimera studies and site-directed mutagenesis, the Y14N substitution in AGM clone 1 CCR5 was solely responsible for blocking HIV-1 infections. In contrast, both AGM CCR5 clones were active coreceptors for SIV mac251. Studies of DNA samples from other AGMs indicated frequent additional CCR5 polymorphisms, and we cloned an AGM clone 2 variant with a Q93R substitution in the extracellular loop 1 from one heterozygote. This variant CCR5 was active as a coreceptor for SIV mac251 but was only weakly active for macrophage-tropic isolates of HIV-1. In addition, SIV mac251 appeared to be dependent on the extracellular amino terminus and loop 2 regions of human CCR5 for maximal infection. Our results suggest major differences in the interactions of SIV mac251 and macrophage-tropic HIV-1 isolates with I9, N13, and Y14 in the amino terminus; with Q93 in extracellular loop 1; and with extracellular loop 2 of human CCR5. (ii) The NIH/Swiss mouse CCR5 protein differs at multiple positions from sequences recently reported for other inbred strains of mice. This CCR5 was inactive as a coreceptor for HIV-1 and SIV mac251. Studies of chimeras that contained different portions of NIH/Swiss mouse CCR5 substituted into human CCR5, as well as the reciprocal chimeras, indicated that the amino-terminal region and extracellular loops 1 and 2 of human CCR5 contribute to its coreceptor activity for macrophage-tropic isolates of HIV-1. Specific differences with previous CCR5 chimera results occurred because the NIH/Swiss mouse CCR5 contains a unique substitution corresponding to P183L in extracellular loop 2 that is nonpermissive for coreceptor activity. We conclude that diverse CCR5 sequences occur in AGMs and mice, that SIV mac251 and macrophage-tropic HIV-1 isolates interact differently with specific CCR5 amino a...

show abstract

“…Preparations of human enteroviruses, in general, are known to contain two distinct antigens as revealed by complement fixation and gel diffusion tests : N (or D) antigen associated with the infectious complete particle and H (or C) antigen characteristic of the noninfectious empty capsid (Roizman et al, 1958;LeBouvier, 1959;Schmidt et al, 1963;Forsgren, 1969). Similarly, a preparation of bovine enterovirus grown in tissue culture was also shown to contain two distinct virus particles, an infectious virion and an empty particle (Johnston and Martin, 1971).…”

Section: Discussionmentioning

confidence: 99%

Properties of Human Serum Factors Precipitating Bovine Enterovirus

Urasawa¹,

Urasawa²,

Kanamitsu³

1973

JJMSB

View full text Add to dashboard Cite

SUMMARY :We investigated the nature of a normal human serum factor (s) precipitating bovine enterovirus, and obtained the following results.(1) The incidence of the precipitating factor in human serum increased greatly at the ages between 2-5. (2) The precipitating factors in most of the sera examined were identified as IgG globulin.(3) A bovine enterovirus was found to possess two distinct antigens resembling N (native) and H (heat stable) antigens of human enteroviruses ; normal human serum reacted exclusively with the latter . (4) The majority of human sera precipitating heat stable antigen of bovine enterovirus also reacted with the same antigens of human enteroviruses, coxsackievirus B1, coxsackievirus B5 and echovirus type 1. (5) Absorption experiments of human serum with heated viruses indicated the presence of a common antigen (s) between the heat stable antigen of bovine enterovirus and those of coxsackie-and echoviruses, while no serological relationship was found between the heat stable antigen of bovine enterovirus and those of polioviruses.From these results it was presumed that the human serum factor precipitating bovine enterovirus is possibly an antibody produced by infection with coxsackie or echoviruses sharing a common antigen (s) with bovine enterovirus.

show abstract

The Sensitivity of Grivet Monkey Kidney Cell Line Bs-C-1 for Propagation And Isolation of Certain Human Viruses

Cited by 11 publications

References 14 publications

Biological Characteristics and Viral Spectrum of Serially Cultivated Fetal Rhesus Monkey Kidney Cells

Biological Characteristics and Viral Spectrum of Serially Cultivated Fetal Rhesus Monkey Kidney Cells

Polymorphisms in the CCR5 genes of African green monkeys and mice implicate specific amino acids in infections by simian and human immunodeficiency viruses

Properties of Human Serum Factors Precipitating Bovine Enterovirus

Contact Info

Product

Resources

About