The sequences of the plastid gene rpl16 and the rpl16-rpl14 spacer region allow discrimination among six species of Scutellaria

Hosokawa, Keizo; Minami, Motoyasu; Nakamura, Ikuo; Hishida, Atsuyuki; Sato, Toshiro

doi:10.1016/j.jep.2004.02.031

Cited by 14 publications

(9 citation statements)

References 8 publications

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“…From chloroplast DNA, a couple of markers including genes, intergenic spacers, or introns are applied. The atpB-rbcL region was used for differentiation of Phyllanthus [138], trnC-trnD in Panax [139], trnL-F in Pueraria [140], Rheum [141] and Ephedra [142], rpl16 in Swertia [143], rpl16-rpl14 spacer in Scutellaria [144], atpF-atpA in Angelica [145], trnD-trnT in Dyosma [146], trnK in Actinidia [147], Atractylodes [148] and Curcuma [149], matK in Agastache [150], Panax [151], rbcL in Dryopteris [152], Cnidium [153], and Pinellia [154]. CAPS or PCR-RFLP (cleaved amplified polymorphic sequence): CAPS, originally named PCR-RFLP is a combination of PCR of target DNA and subsequent digestion with a restriction enzyme [155,156].…”

Section: Pcr-based Methodsmentioning

confidence: 99%

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Heubl¹

2010

Planta Med

136

104

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Section: Pcr-based Methodsmentioning

confidence: 99%

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Heubl¹

2010

Planta Med

136

104

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“…Other species of Scutellaria that may be mixed or substituted for S. lateriflora include: S. incana, S. galericulata, and the Chinese species S. baicalensis, of which the root is typically used. Numerous methods are available for differentiating between closely related Scutellaria and Teucrium species including genetic sequencing of Scutellaria species (Hosokawa et al, 2005) and physical and chemical analyses (Avula et al, 2003;Bedir et al, 2003;Gafner et al, 2003;Peck et al, 1993).…”

Section: Adulterantsmentioning

confidence: 99%

Skullcap Scutellaria lateriflora L.: An American nervine

Upton¹,

DAyu²

2012

Journal of Herbal Medicine

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“…The washed DNA was air-dried and then dissolved in 100 ll of TE buffer. The concentration of extracted DNA was adjusted to 20-40 ng/ll [13]. Detection of DNA polymorphisms in the rpl16 gene and the rpl16-rpl14 spacer region in the chloroplast DNA [14] was performed as described previously [13].…”

Section: Morphological Characteristics Of Flower Headsmentioning

confidence: 99%

“…The authors focused on the sequence of the rpl16 gene and the rpl16-rpl14 spacer region, because it has been proposed that such sequences might be useful for the identification of higher plants, as demonstrated, for example, in Scutellaria [13] and Oryza sativa [14]. Nucleotide sequences (534-552 bp) were obtained for all specimens by direct sequencing (Table 2).…”

Section: Cap and Dme Contents Of Flower Headsmentioning

confidence: 99%

Preliminary survey of taxonomical problems, pharmacognostical characteristics, and chloroplast DNA polymorphisms of the folk medicinal herb Artemisia campestris from the Ryukyu Islands, Japan

et al. 2010

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Artemisia campestris L. (Compositae) occurs naturally along the coastline of the Ryukyu Islands and has been traditionally used as a folk medicine for the treatment of liver and kidney disorders. The authors obtained specimens from the Ishigaki and Kume Islands of the Ryukyu Islands, Japan, and from the USA. A survey of the literature revealed that the Japanese name for A. campestris is Niitaka-yomogi or Riukiu-yomogi. Two distinct overall plant-form phenotypes were identified: an erect phenotype with long, upright, and straight main axis and assurgent branches; and a prostrate phenotype, having branches that are longer than the main axis and which grow along the ground. Except for the number of ray flowers, most of the flower head characters in the erect phenotypes were significantly larger than those in the prostrate phenotypes. In this experiment, the flower heads contained only small amounts of either capillarisin (<0.01-0.11 of the dry weight, % DW) and 6,7-dimethylesculetin (<0.01-0.30% DW), or none at all. DNA polymorphisms at two sites of the rpl16-rpl14 spacer region (nucleotide position 181-189 and 291-300 from the 5' end) revealed the existence of four different haplotypes. The number of adenines at nucleotide positions 291-300 appeared to be polymorphic within A. campestris from the Ryukyu Islands. Conversely, geographic differences between specimens from the Ryukyu Islands and USA manifested as a nine-base deletion at nucleotide positions 181-189. From a pharmacognostical context, the use of A. campestris flower heads as a substitute for Artemisiae capillaris Flos is not effective.

show abstract

The sequences of the plastid gene rpl16 and the rpl16-rpl14 spacer region allow discrimination among six species of Scutellaria

Cited by 14 publications

References 8 publications

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Skullcap Scutellaria lateriflora L.: An American nervine

Preliminary survey of taxonomical problems, pharmacognostical characteristics, and chloroplast DNA polymorphisms of the folk medicinal herb Artemisia campestris from the Ryukyu Islands, Japan

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