The Sertoli cell of the water buffalo (Bubalus bubalis) during the spermatogenic cycle

Pawar, Harcharan Singh; Wrobel, Karl‐Heinz

doi:10.1007/bf00318137

Cited by 19 publications

(7 citation statements)

References 19 publications

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“…Therefore our material was collected from 16-to 17-month-old yearlings shot in septemberbctober during the pre-rut of the adult population. The volume density of testicular tubules in the fallow deer is 72.2% and thus somewhat smaller than in other ruminant testes as bull: 85% (Dostal, 1988), ram: 85% (Schmidt, 1989), water buffalo: 82% (Pawar and Wrobel, 1991a). Tubular diameter averages 220 pm in the fallow deer and is distinctly smaller than in other young adult ruminants.…”

Section: Discussionmentioning

confidence: 83%

Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Wrobel

Kessler

Schimmel

1993

Reprod Domestic Animals

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Contents: The quantitative morphology of the seminiferous epithelium was studied in 10 testes of 16‐ to 17‐monthsold fallow bucks (Dama dama). Seminiferous tubules constitute 77.2% of the testicular parenchyma and amount to a total length of 400–750 m per testis. According to varying cell associations 6 different stages can be observed during the spermatogenic cycle. The average tubular diameter varies between 215 μm (stage 1) and 230 μm (stage 8), the epithelial height between 67.6 μm in stage 3 and 71.9 μm in stage 8 when spermiation occurs. From primary spermatocytes during the prophase of the first meiotic division preleptotenes have smallest nuclear (203.7 μm3) and cellular (469.1 μm3) volumes, whereas late diplotenes show a nuclear volume of 846.7μm3 and a cellular volume of 3440.5 μm3, that is an increase of more than 4 times for the nucleus and more than 7 times for the cell body. Numerical analysis reveals 4 spermatogonial divisions and a considerable loss of spermatogenic cells during the second meiotic division and subsequent spermiogenesis. Sertoli cells constitute between 31.4% (stage 4) and 38.3% (stage 1) of the tubular epithelium. The calculated cell volume of an individual Sertoli cell increases during every cycle from 6515.3 μm3 instage 3 to 8350.0 μm3 in stage 8; that is a volume change of 22%. The Sertoli cell organelle with most prominent cyclical variations is the endoplasmic reticulum. A lipid cycle is absent in the seminiferous epithelium of the fallow deer. Inhalt: Quantitative Studien am Keimepithel des männlichen Damwilds (Dama dama). Das Keimepithel im Hoden von fünf 16 bis 17 Monate alten Damwildjährlingen (Dama dama) wurde mit morphometrischen Methoden untersucht. Tubuli seminiferi machen 77,2% des Hodenparenchyms aus; die Gesamttubuluslange pro Hoden betragt zwischen 400 und 750 m. Sechs Spermatogenesestadien können beim Damhirsch identifiziert werden, wobei für diese Einteilung wechselnde Zellassoziationen im Keimepithel zugrunde gelegt wurden. Der Durchmesser der Tubuli seminiferi wechselt zwischen 215 μm (Phase 1)und 230 μm (Phase 8). Die Tubulusepithelhöhe schwankt ebergfalls zwischen 67,6 μm in Phase 3 und 71,9 μm in Phase 8 (Spermiation). Präleptotäne habendie kleinsten Kern‐ und Zellvolumina (203,7 μm3 bzw. 469,1 μm3) von allen Spermatozyten I. Ordnung, Diplotäne aus Phase 4 die größten Werte (846,7 μm3 bzw. 3440,5 μm3), das ist ein Zuwachs um das Vierfache bei mKern und um das Siebenfache beim Zeilleib. Eine numerische Anaiyse derZellzahlen in Tubulusquerschnitten aus den verschiedenen Phasen der Spermatogenese erlaubt die Schlußfolgerung, daß es 4 Spermatogonienteilungen bei dieser Spezies gibt, und daß eine beträchtliche Anzahl aller rechnerisch möglichen Keimzellen während der Spermatokenese verloren geht, vor allem während der zweiten Reifeteilung und der Spermiogenese. Sertolizellen machen zwischen 31,4 (Phase 4) und 38,3% (Phase 1) des Keimepithels aus. Eine Sertolizelle aus Phase 3 besitzt ein rechnerisches Zellvolumen von 6515,3 μm3, eine Zelle aus Phase 8 ein Zell...

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Section: Discussionmentioning

confidence: 83%

Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Wrobel

Kessler

Schimmel

1993

Reprod Domestic Animals

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“…In this current study, the nuclei existed away from the basal membrane during hibernation and early spermatogenesis and were located near the basal membrane during late spermatogenesis. The nucleolus in ruminant SCs is heavily vacuolated or multivesiculated (Pawar and Wrobel ), but no vacuoles were observed in the nucleolus of the Chinese soft‐shelled turtle at any stage.…”

Section: Discussionmentioning

confidence: 99%

Cytological study on Sertoli cells and their interactions with germ cells during annual reproductive cycle in turtle

Ahmed

Huang

Yang

et al. 2016

Ecology and Evolution

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Sertoli cells (SCs) play a central role in the development of germ cells within functional testes and exhibit varying morphology during spermatogenesis. This present study investigated the seasonal morphological changes in SCs in the reproductive cycle of Pelodiscus sinensis by light microscopy, transmission electron microscopy (TEM), and immunohistochemistry. During hibernation period with the quiescent of spermatogenesis, several autophagosomes were observed inside the SCs, the processes of which retracted. In early spermatogenesis, when the germ cells started to proliferate, the SCs contained numerous lipid droplets instead of autophagosomes. In late spermatogenesis, the SCs processes became very thin and contacted several round/elongated spermatids in pockets. At this time, abundant endoplasmic reticulum and numerous mitochondria were present in the SCs. The organization of the tight junctions and the adherens junctions between the SCs and germ cells also changed during the reproductive cycle. Moreover, SCs were involved in the formation of cytoplasmic bridges, phagophores, and exosome secretions during spermatogenesis. Tubulobulbar complexes (TBC) were also developed by SCs around the nucleus of the spermatid at the time of spermiation. Strong, positive expression of vimentin was noted on the SCs during late spermatogenesis compared with the hibernation stage and the early stage of spermatogenesis. These data provide clear cytological evidence about the seasonal changes in SCs, corresponding with their different roles in germ cells within the Chinese soft‐shelled turtle Pelodiscus sinensis.

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“…This is evidenced for many cellular activities such as secretion of specific proteins (Griswold 1995), apoptotic processes (Kerr 1992;Blanco-Rodriguez and Martinez-Garcia 1996) or the gene expression (Yu et al 2003). Sertoli cells, especially, show stagespecific variations in structure (Ueno andMori 1990, Pawar andWrobel 1991) and function (Griswold 1998). However, cell-specific expression and distribution of many factors is equivalent to seminiferous epithelium stages only for steady state spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Histological organization of roe deer testis throughout the seasonal cycle: variable and constant components of tubular and interstitial compartment

Schön

Göritz

Streich

et al. 2004

Anatomy and Embryology

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Seasonally regulated breeding in roe deer, Capreolus capreolus, is associated with significant changes in testis mass, structure and function. This study has quantified seasonal changes of morphometric parameters and cellular composition in roe deer testis parenchyma. Tissue samples were collected bimonthly during a complete annual cycle. Morphometric parameters of seminiferous tubules were measured and the number of different cell types was counted using a computer-aided image-analyzing system. A scheme of eight tubular epithelium stages for active spermatogenesis was devised according to the spermatid development. Stage I is characterized by the occurrence of new round spermatids, stage IV by spermiation and stage VIII by the meiotic division of spermatocytes. The average diameter of seminiferous tubules varied between 88.4+/-3.6 micro m (February) and 216.8+/-9.2 micro m (June). Also numbers of spermatogonia, spermatocytes and spermatids per tubule cross-section showed considerable seasonal changes. In December and February the germinative epithelium mainly consists of Sertoli cells and spermatogonia. In February, the first differentiated spermatogonia enter meiosis, and in April even spermatids occasionally occur, which reach their highest numbers during the rut in August. Both the expansion and the proportion of tubular and interstitial compartment change seasonally and result in differing cell densities. Assuming numerically constant populations of Sertoli cells and interstitial cells during the entire year, the hypothetical cell numbers per mm(2) of the tubular and interstitial areas were calculated for the seasonally variable total areas of tissue cross-sections. The concordance of these theoretical values with measured cell densities provided evidence that the total numbers of Sertoli cells, as well as interstitial cells, remain really constant throughout the seasonal cycle. The exact quantification of variable and constant components provides basic data for characterization of cell type and stage-specific processes of spermatogenesis.

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The Sertoli cell of the water buffalo (Bubalus bubalis) during the spermatogenic cycle

Cited by 19 publications

References 19 publications

Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Quantitative Evaluations of the Tubular Epithelium in the Testis of the Fallow Deer (Dama dama)

Cytological study on Sertoli cells and their interactions with germ cells during annual reproductive cycle in turtle

Histological organization of roe deer testis throughout the seasonal cycle: variable and constant components of tubular and interstitial compartment

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