The sheep as a model for healing studies after partial nephrectomy

Souza, Diogo Benchimol de; Costa, Waldemar S.; Damasceno-Ferreira, José Aurelino; Júnior, Amary Nascimento; Ascoli, F. O.; Pereira‐Sampaio, Marco A.; Sampaio, Francisco J.B.

doi:10.1016/j.jss.2015.07.013

Cited by 7 publications

(9 citation statements)

References 26 publications

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“…The present study depicted histological similarities between the ovine and human collecting systems, which supports the findings of De Souza et al (). Specifically, it seems that the densities of blood vessels and elastic fibers are quite similar between these two species.…”

Section: Discussionsupporting

confidence: 93%

“…Thus, these authors reported that the swine should not be used for studying collecting system healing. Moreover, a recent study showed that the ovine kidney heals in a manner more similar to that in humans, after partial nephrectomy with collecting system opening, resulting in urinoma and urinary fistulas (de Souza et al, 2016). The present study depicted histological similarities between the ovine and human collecting systems, which supports the findings of De Souza et al (2016).…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

“…Ames et al () suggested that the lack of renal fascia (Gerota's fascia) and the low amount of perinephric fat observed in swine could contribute to the closure of the collecting system. It was later observed that sheep have a tissue around the kidney that resembles the renal fascia (de Souza et al, ). This could explain the similarities between ovine and human kidney healing after partial nephrectomy.…”

Section: Discussionmentioning

confidence: 99%

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Histomorphometric comparison of the human, swine, and ovine collecting systems

Simões

Souza

Gallo

et al. 2016

The Anatomical Record

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The ovine kidney has been recently determined to be a better model than the swine kidney for the study of collecting system healing after partial nephrectomy. However, there is no histological study comparing the collecting systems of these species. To compare human, swine, and ovine collecting systems using histomorphometry. The collecting systems of 10 kidneys from each species (human, swine, and ovine) were processed for histomorphometry. The thickness of the three layers (mucosal connective tissue, submucosal muscular, and adventitial connective tissue) were measured. The densities of smooth muscle fibers, elastic system fibers, and cells were also measured. Additionally, blood vessel density in the adventitial connective tissue was measured. Analysis of the collecting systems from the three species presented several differences. The adventitial connective tissue from the swine samples was thicker, with more blood vessels and smooth muscle fibers, compared with that from the human and ovine samples. Swine also had higher density of elastic fibers on the submucosal muscular layer. Ovine and human collecting systems shared several similar features, such as blood vessel and elastic fiber density in all layers and the density of cellular and muscular fibers in the submucosal muscular and adventitial connective tissue layers. The collecting system of the ovine kidney is more similar to that of the human kidney compared with that of the swine kidney. This may explain the differences between the healing mechanisms in swine and those in humans and sheep after partial nephrectomy. Anat Rec, 299:967-972, 2016. V C 2016 Wiley Periodicals, Inc.Key words: animal models; collecting system; healing; kidney; partial nephrectomySwine has been considered the best animal model for studying partial nephrectomy, because this species bears anatomical and physiological similarities to humans (Sampaio et al., 1998;Yang et al., 2009;de Souza et al., 2011). However, it has been reported that healing in the swine kidney is quite different from the clinical setting in humans. No urinary leakage was observed in swine after laparoscopic partial nephrectomy without collecting system closure (Ames et al., 2005;de Souza et al., 2011), whereas urinary leakage occurred in up to 5.5% of patients, regardless of the collecting system closure method (Stephenson et al., 2004

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Histomorphometric comparison of the human, swine, and ovine collecting systems

Simões

Souza

Gallo

et al. 2016

The Anatomical Record

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“…Previous studies on partial nephrectomy without closure have demonstrated that the pig kidney collecting system heals different from humans, without fistula or urinoma (Ames et al, ; De Souza et al, ). Recently, a study in sheep demonstrated that partial nephrectomy without closure of the collecting system resulted in urinary leakage and urinoma (De Souza et al, ). Thus, sheep could be a more suitable model than pigs for studies on kidney collecting system healing.…”

mentioning

confidence: 99%

Anatomical Relationship Between the Kidney Collecting System and the Intrarenal Arteries in the Sheep: Contribution for a New Urological Model

Buys-Gonçalves¹,

Souza²,

Sampaio³

et al. 2016

The Anatomical Record

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Previous studies have demonstrated that the pig collecting system heals after partial nephrectomy without closure. Recently, a study in sheep showed that partial nephrectomy without closure of the collecting system resulted in urinary leakage and urinoma. The aim of this study was to present detailed anatomical findings on the intrarenal anatomy of the sheep. Forty two kidneys were used to produce tridimensional endocasts of the collecting system together with the intrarenal arteries. A renal pelvis which displayed 11-19 (mean of 16) renal recesses was present. There were no calices present. The renal artery was singular in each kidney and gave two primary branches one to the dorsal surface and one to ventral surface. Dorsal and ventral branches of the renal artery were classified based on the relationship between their branching pattern and the collecting system as: type I (cranial and caudal segmental arteries), type II (cranial, middle and caudal segmental arteries) or type III (cranial, cranial middle, caudal middle, and caudal segmental arteries). Type I was the most common branching pattern for the dorsal and ventral branches of the renal artery. The arterial supply of the caudal pole of the sheep kidney supports its use as an experimental model due to the similarity to the human kidney. However, the lack of a retropelvic artery discourages the use of the cranial pole in experiments in which the arteries are an important aspect to be considered. Anat Rec, 299:405-411, 2016. V C 2016 Wiley Periodicals, Inc.

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