The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure

Lee, S.-H.; Lee, Changhyoup; Lee, K.W.; Cho, H.B.; Kim, S.-J.

doi:10.1111/j.1365-2672.2004.02496.x

Cited by 67 publications

(47 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The authors detected EV in 57% of surface water samples, while only 37% of samples were positive for AdV. Slightly higher EV detection was also related to Lee et al (2005), who detected EV in 33.3% of surface water samples collected from Han River (Seoul, Korea) and AdV in 30.4% of samples. Chapron et al (2000) verified a similar situation, with higher EV detection (58.6%) than AdV (48.3%) in surface water samples.…”

Section: Discussionmentioning

confidence: 98%

First description of Adenovirus, Enterovirus, Rotavirus and Torque teno virus in water samples collected from the Arroio Dilúvio, Porto Alegre, Brazil

et al. 2012

View full text Add to dashboard Cite

Adenovirus (AdV), enterovirus (EV), genogroup A rotaviruses (GARV) and Torque teno virus (TTV) are non-enveloped viral agents excreted in feces and so may contaminate water bodies. In the present study, the molecular detection of these viruses was performed in samples of surface water collected from the Arroio Dilúvio, a waterstream that crosses the city of Porto Alegre, RS, Brazil, receiving great volumes of non-treated sewage from a large urban area. Sampling was performed during 2009, in three different occasions (January, April and September). The highest detection rate was observed for EV (64.28%), followed by TTV (28.57%) and AdV (21.43%). Rotaviruses were not detected. More than on kind of tested virus was detected in five (35. 71%) of 14 samples. January was the month with the highest viral detection rate, being all samples, collected in this month, positive for at least one group of tested virus. The correlation between the detection of these different viral agents and environmental factors is discussed. To the knowledge of the authors, this is the first description of viral genomes in water samples taken from the Arroio Dilúvio, Porto Alegre (Brazil).

show abstract

Section: Discussionmentioning

confidence: 98%

First description of Adenovirus, Enterovirus, Rotavirus and Torque teno virus in water samples collected from the Arroio Dilúvio, Porto Alegre, Brazil

et al. 2012

View full text Add to dashboard Cite

show abstract

“…However, PCR results may not reflect the infectivity of the samples since PCR only detects the genes of the pathogens; therefore, integrated cell culture-PCR (ICC-PCR) was developed to identify the specific infectious enteric viruses. ICC-PCR has been used to detect infectious enteric viruses in river water, tap water, beach water, and wastewater effluent samples (28,29,39,50). However, Buffalo green monkey (BGM) cell culture, currently recommended by the EPA, has been compared with other cell lines, such as A549 and PLC/PRC/5 (28,39), and the results showed that enteric viruses were propagated better with these cell lines than with BGM cells.…”

mentioning

confidence: 99%

Quantification of Enteric Viruses, Pathogen Indicators, and Salmonella Bacteria in Class B Anaerobically Digested Biosolids by Culture and Molecular Methods

Wong

Onan

Xagoraraki

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

The most common class B biosolids in the United States are generated by mesophilic anaerobic digestion (MAD), and MAD biosolids have been used for land application. However, the pathogen levels in MAD biosolids are still unclear, especially with respect to enteric viruses. In this study, we determined the occurrence and the quantitative levels of enteric viruses and indicators in 12 MAD biosolid samples and of Salmonella enterica in 6 MAD biosolid samples. Three dewatered biosolid samples were also included in this study for purposes of comparison. Human adenoviruses (

show abstract

“…Although HAdVs have frequently been identified in various environments such as wastewater (19,22,39,41), drinking waters (20,30,31,55), groundwater (28,40), surface waters (7,15,17,25,42,44), and pool recreational waters (35,38,50,54), quantitative information for HAdV occurrence at public lake beaches is still insufficient. The overall goal of this study was to gain insight into HAdV abundance in freshwaters at two popular recreational beaches on Lake Michigan using quantitative PCR assays.…”

mentioning

confidence: 99%

Occurrence of Human Adenoviruses at Two Recreational Beaches of the Great Lakes

Xagoraraki¹,

Kuo²,

Wong³

et al. 2007

Appl Environ Microbiol

109

View full text Add to dashboard Cite

Human adenoviruses (HAdVs) have been related to several waterborne diseases such as acute gastroenteritis, conjunctivitis, and respiratory illness, and it has been shown that an important human exposure pathway is through recreational waters. However, HAdV occurrence at recreational freshwater beaches has not been previously investigated. In this study, a total of 58 water samples were collected from two recreational beaches on Lake Michigan (

show abstract

The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure

Cited by 67 publications

References 45 publications

First description of Adenovirus, Enterovirus, Rotavirus and Torque teno virus in water samples collected from the Arroio Dilúvio, Porto Alegre, Brazil

First description of Adenovirus, Enterovirus, Rotavirus and Torque teno virus in water samples collected from the Arroio Dilúvio, Porto Alegre, Brazil

Quantification of Enteric Viruses, Pathogen Indicators, and Salmonella Bacteria in Class B Anaerobically Digested Biosolids by Culture and Molecular Methods

Occurrence of Human Adenoviruses at Two Recreational Beaches of the Great Lakes

Contact Info

Product

Resources

About